Advanced SearchSearch Tips
Evaluation of Relative Bioavailability of 25-Hydroxycholecalciferol to Cholecalciferol for Broiler Chickens
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Evaluation of Relative Bioavailability of 25-Hydroxycholecalciferol to Cholecalciferol for Broiler Chickens
Han, J.C.; Chen, G.H.; Wang, J.G.; Zhang, J.L.; Qu, H.X.; Zhang, C.M.; Yan, Y.F.; Cheng, Y.H.;
  PDF(new window)
This study was conducted to evaluate the relative bioavailability (RBV) of 25-hydroxycholecalciferol (25-OH-) to cholecalciferol (vitamin ) in 1- to 21-d-old broiler chickens fed with calcium (Ca)- and phosphorus (P)-deficient diets. On the day of hatch, 450 female Ross 308 broiler chickens were assigned to nine treatments, with five replicates of ten birds each. The basal diet contained 0.50% Ca and 0.25% non-phytate phosphorus (NPP) and was not supplemented with vitamin D. Vitamin was fed at 0, 2.5, 5.0, 10.0, and , and 25-OH- was fed at 1.25, 2.5, 5.0, and . The RBV of 25-OH- was determined using vitamin as the standard source by the slope ratio method. Vitamin and 25-OH- intake was used as the independent variable for regression analysis. The linear relationships between the level of vitamin or 25-OH- and body weight gain (BWG) and the weight, length, ash weight, and the percentage of ash, Ca, and P in femur, tibia, and metatarsus of broiler chickens were observed. Using BWG as the criterion, the RBV value of 25-OH- to vitamin was 1.85. Using the mineralization of the femur, tibia, and metatarsus as criteria, the RBV of 25-OH- to vitamin ranged from 1.82 to 2.45, 1.86 to 2.52, and 1.65 to 2.05, respectively. These data indicate that 25-OH- is approximately 2.03 times as active as vitamin in promoting growth performance and bone mineralization in broiler chicken diets.
25-Hydroxycholecalciferol;Cholecalciferol;Relative Bioavailability;Growth;Bone;Broiler Chicken;
 Cited by
Aburto, A., H. M. Edwards Jr., and W. M. Britton. 1998. The influence of vitamin A on the utilization and amelioration of toxicity of cholecalciferol, 25-hydroxycholecalciferol, and 1,25 dihydroxycholecalciferol in young broiler chickens. Poult. Sci. 77:585-593. crossref(new window)

Applegate, T. J. and M. S. Lilburn. 2002. Growth of the femur and tibia of a commercial broiler line. Poult. Sci. 81:1289-1294. crossref(new window)

Atencio, A., G. M. Pesti, and H. M. Edwards. Jr. 2005. Twenty-five hydroxycholecalciferol as a cholecalciferol substitute in broiler breeder hen diets and its effect on the performance and general health of the progeny. Poult. Sci. 84:1277-1285. crossref(new window)

Baker, D. H., R. R. Biehl, and J. L. Emmert. 1998. Vitamin $D_3$ requirement of young chicks receiving diets varying in calcium and available phosphorus. Br. Poult. Sci. 39:413-417. crossref(new window)

Bar, A., D. Shinder, S. Yosefi, E. Vax, and I. Plavnik. 2003. Metabolism and requirements for calcium and phosphorus in the fast-growing chicken as affected by age. Br. J. Nutr. 89:51-60. crossref(new window)

Biehl, R. R. and D. H. Baker. 1997. Utilization of phytate and nonphytate phosphorus in chicks as affected by source and amount of vitamin $D_3$. J. Anim. Sci. 75:2986-2993. crossref(new window)

Edwards Jr., H. M. 2002. Studies on the efficacy of cholecalciferol and derivatives for stimulating phytate utilization in broilers. Poult. Sci. 81:1026-1031. crossref(new window)

Fritts, C. A. and P. W. Waldroup. 2003. Effect of source and level of vitamin D on live performance and bone development in growing broilers. J. Appl. Poult. Res. 12:45-52. crossref(new window)

Goetting-Fuchs, C., R. Gunther, V. G. Liesner, B. G. Liesner, M. Beyerbach, and J. Kamphues. 2012. Investigations on skeletal development, bone mineralisation as well as calcium and phosphorus levels in blood of male fattening turkeys. Europ. Poult. Sci. 76:121-130.

Goodgame, S. D., F. J. Mussini, C. Lu, C. D. Bradley, S. E. Watkins, and P. W. Waldroup. 2011. Evaluation of a fermentation source of 25-hydroxycholecalciferol in broiler diets. Int. J. Poult. Sci. 10:295-299. crossref(new window)

Hall, L. E., R. B. Shirley, R. I. Bakalli, S. E. Aggrey, G. M. Pesti, and H. M. Edwards. Jr. 2003. Power of two methods for the estimation of bone ash of broilers. Poult. Sci. 82:414-418. crossref(new window)

Han, J. C., H. X. Qu, J. G. Wang, G. H. Chen, Y. F. Yan, J. L. Zhang, F. M. Hu, L. Y. You, and Y. H. Cheng. 2015. Comparison of the growth and mineralization of the femur, tibia, and metatarsus of broiler chicks. Braz. J. Poult. Sci. 17:333-339. crossref(new window)

Han, J. C., H. X. Qu, J. Q. Wang, J. H. Yao, C. M. Zhang, G. L. Yang, Y. H. Cheng, and X. S. Dong. 2013. The effects of dietary cholecalciferol and $1{\alpha}$-hydroxycholecalciferol levels in a calcium- and phosphorus-deficient diet on growth performance and tibia quality of growing broilers. J. Anim. Feed Sci. 22:158-164. crossref(new window)

Han, J. C., Y. Liu, J. H. Yao, J. Q. Wang, H. X. Qu, Y. F. Yan, J. Yue, J. L. Ding, Z. T. Shi, and X. S. Dong. 2012. Dietary calcium levels reduce the efficacy of one alpha-hydroxycholecalciferol in phosphorus-deficient diets of broilers. J. Poult. Sci. 49:34-38. crossref(new window)

Jendral, M. J., D. R. Korver, J. S. Church, and J. J. R. Feddes. 2008. Bone mineral density and breaking strength of white leghorns housed in conventional, modified, and commercially available colony battery cages. Poult. Sci. 87:828-837. crossref(new window)

Koreleski, J. and S. Swiatkiewicz. 2005. Efficacy of different limestone particle size and 25-hydroxycholecalciferol in broiler diets. J. Anim. Feed Sci. 14:705-714. crossref(new window)

Ledwaba, M. F. and K. D. Roberson. 2003. Effectiveness of twenty five hydroxycholecalciferol in the prevention of tibial dyschondroplasia in Ross cockerels depends on dietary calcium level. Poult. Sci. 82:1769-1777. crossref(new window)

Littell, R. C., P. R. Henry, A. J. Lewis, and C. B. Ammerman. 1997. Estimation of relative bioavailability of nutrients using SAS procedures. J. Anim. Sci. 75:2672-2683. crossref(new window)

Qian, H., E. T. Kornegay, and D. M. Denbow. 1997. Utilization of phytate phosphorus and calcium as influenced by microbial phytase, cholecalciferol, and the calcium: Total phosphorus ratio in broiler diets. Poult. Sci. 76:37-46. crossref(new window)

Rao, S. V. R., M. V. L. N. Raju, A. K. Panda, G. S. Sunder, and R. P. Sharma. 2006. Effect of high concentrations of cholecalciferol on growth, bone mineralization, and mineral retention in broiler chicks fed suboptimal concentrations of calcium and nonphytate phosphorus. J. Appl. Poult. Res. 15:493-501. crossref(new window)

Rao, S. V. R., M. V. L. N. Raju, A. K. Panda, G. S. Sunder, and R. P. Sharma. 2009. Performance and bone mineralisation in broiler chicks fed on diets with different concentrations of cholecalciferol at a constant ratio of calcium to non-phytate phosphorus. Br. Poult. Sci. 50:528-535. crossref(new window)

Rao, S. V. R., M. V. L. N. Raju, and M. R. Reddy. 2007. Performance of broiler chicks fed high levels of cholecalciferol in diets containing sub-optimal levels of calcium and non-phytate phosphorus. Anim. Feed Sci. Tech. 134:77-88. crossref(new window)

SAS Institute. 2002. SAS User's Guide. 9th edn. SAS Inst. Inc., Cary, NC, USA.

Shirley, R. B. 2003. Evaluation of Phytase, Vitamin D3 Derivatives, and Broiler Breed Differences on Nutrient Utilization, Broiler Performance, Leg Disorders, and the Expression of Intestinal Calbindin-28 kd mRNA and Protein. Ph.D. Dissertation. University of Georgia, Athens, GA, USA.

Soares Jr., J. H., J. M. Kerr, and R. W. Gray. 1995. 25-hydroxycholecalciferol in poultry nutrition. Poult. Sci. 74:1919-1934. crossref(new window)

Yarger, J. G., C. A. Saunders, J. L. McNaughton, C. L. Quarles, B. W. Hollis, and R. W. Gray. 1995. Comparison of dietary 25-hydroxycholecalciferol and cholecalciferol in broiler chickens. Poult. Sci. 74:1159-1167. crossref(new window)