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Inhibition of MMP-2 and MMP-9 Activities by Limonium tetragonum Extract
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  • Journal title : Preventive Nutrition and Food Science
  • Volume 21, Issue 1,  2016, pp.38-43
  • Publisher : The Korean Society of Food Science and Nutrition
  • DOI : 10.3746/pnf.2016.21.1.38
 Title & Authors
Inhibition of MMP-2 and MMP-9 Activities by Limonium tetragonum Extract
Bae, Min-Joo; Karadeniz, Fatih; Lee, Seul-Gi; Seo, Youngwan; Kong, Chang-Suk;
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 Abstract
Matrix metalloproteinases (MMPs) are crucial extracellular matrices degrading enzymes that take important roles in metastasis of cancer progression as well as other significant conditions such as oxidative stress and hepatic fibrosis. Natural products are on the rise for their potential to provide remarkable health benefits. In this context, halophytes have been of interest in the nutraceutical field with reported instances of isolation of bioactive compounds. In this study, Limonium tetragonum, an edible halophyte, was studied for its ability to inhibit MMP-2 and -9 using HT1080 fibrosarcoma cells. Results showed that L. tetragonum extract was able to inhibit the enzymatic activity and mRNA expression of MMP-2 and -9 according to gelatin zymography and RT-PCR assays, respectively, but it was not able to significantly change the MMP pathway related factors such as tissue inhibitors of metalloproteinases. Also, Mitogen-activated protein kinases pathway-related protein levels and their phosphorylation were assayed. While the phosphorylated p38 levels were decreased, extracellular signal-regulated kinase and c-Jun N-terminal kinase were not affected by L. tetragonum treatment. In conclusion, it was suggested that L. tetragonum contains substances acting as MMP inhibitors on enzymatic activity rather than intracellular pathway intervention, which could be useful for further utilization of L. tetragonum as a source for anti-MMP agents.
 Keywords
HT1080;Limonium tetragonum;MMP;TIMP;MAPK;
 Language
English
 Cited by
1.
ZFP403, a novel tumor suppressor, inhibits the proliferation and metastasis in ovarian cancer, Gynecologic Oncology, 2017  crossref(new windwow)
2.
MMP-Inhibitory Effects of Flavonoid Glycosides from Edible Medicinal Halophyte Limonium tetragonum, Evidence-Based Complementary and Alternative Medicine, 2017, 2017, 1  crossref(new windwow)
3.
Arctigenin, a lignan from Arctium lappa L., inhibits metastasis of human breast cancer cells through the downregulation of MMP-2/−9 and heparanase in MDA-MB-231 cells, Oncology Reports, 2017, 37, 1, 179  crossref(new windwow)
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