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Green Tea (-)-Epigallotocatechin-3-Gallate Induces PGC-1α Gene Expression in HepG2 Cells and 3T3-L1 Adipocytes
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  • Journal title : Preventive Nutrition and Food Science
  • Volume 21, Issue 1,  2016, pp.62-67
  • Publisher : The Korean Society of Food Science and Nutrition
  • DOI : 10.3746/pnf.2016.21.1.62
 Title & Authors
Green Tea (-)-Epigallotocatechin-3-Gallate Induces PGC-1α Gene Expression in HepG2 Cells and 3T3-L1 Adipocytes
Lee, Mak-Soon; Lee, Seohyun; Doo, Miae; Kim, Yangha;
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 Abstract
Green tea (Camellia sinensis) is one of the most popular beverages in the world and has been acknowledged for centuries as having significant health benefits. (-)-Epigallocatechin-3-gallate (EGCG) is the most abundant catechin in green tea, and it has been reported to have health benefit effects. Peroxisome proliferator-activated receptor coactivator is a crucial regulator of mitochondrial biogenesis and hepatic gluconeogenesis. The objective of this study was to investigate whether EGCG from green tea can affect the ability of transcriptional regulation on mRNA expression in HepG2 cells and 3T3-L1 adipocytes. To study the molecular mechanism that allows EGCG to control expression, the promoter activity levels of were examined. The mRNA level was measured using quantitative real-time PCR. The -970/+412 bp of promoter was subcloned into the pGL3-Basic vector that includes luciferase as a reporter gene. EGCG was found to up-regulate the mRNA levels significantly with of EGCG in HepG2 cells and differentiated 3T3-L1 adipocytes. promoter activity was also increased by treatment with of EGCG in both cells. These results suggest that EGCG may induce gene expression, potentially through promoter activation.
 Keywords
EGCG;;promoter activity;HepG2 cells;3T3-L1 adipocytes;
 Language
English
 Cited by
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Involvement of PKCα and ERK1/2 signaling pathways in EGCG’s protection against stress-induced neural injuries in Wistar rats, Neuroscience, 2017, 346, 226  crossref(new windwow)
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Chemoprevention of obesity by dietary natural compounds targeting mitochondrial regulation, Molecular Nutrition & Food Research, 2017, 61, 6, 1600721  crossref(new windwow)
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Peroxisome proliferator-activated receptors (PPARs) as therapeutic target in neurodegenerative disorders, Biochemical and Biophysical Research Communications, 2017, 483, 4, 1166  crossref(new windwow)
 References
1.
Yang CS, Lambert JD, Ju J, Lu G, Sang S. 2007. Tea and cancer prevention: molecular mechanisms and human relevance. Toxicol Appl Pharmacol 224: 265-273. crossref(new window)

2.
Wolfram S, Wang Y, Thielecke F. 2006. Anti-obesity effects of green tea: from bedside to bench. Mol Nutr Food Res 50: 176-187. crossref(new window)

3.
Grove KA, Lambert JD. 2010. Laboratory, epidemiological, and human intervention studies show that tea (Camellia sinensis) may be useful in the prevention of obesity. J Nutr 140: 446-453. crossref(new window)

4.
Lee MS, Kim CT, Kim Y. 2009. Green tea (-)-epigallocatechin-3-gallate reduces body weight with regulation of multiple genes expression in adipose tissue of diet-induced obese mice. Ann Nutr Metab 54: 151-157. crossref(new window)

5.
Lin J, Handschin C, Spiegelman BM. 2005. Metabolic control through the PGC-1 family of transcription coactivators. Cell Metab 1: 361-370. crossref(new window)

6.
Puigserver P. 2005. Tissue-specific regulation of metabolic pathways through the transcriptional coactivator PGC1-$\alpha$. Int J Obes 29: S5-S9. crossref(new window)

7.
Puigserver P, Wu Z, Park CW, Graves R, Wright M, Spiegelman BM. 1998. A cold-inducible coactivator of nuclear receptors linked to adaptive thermogenesis. Cell 92: 829-839. crossref(new window)

8.
Canto C, Auwerx J. 2009. PGC-$1{\alpha}$, SIRT1 and AMPK, an energy sensing network that controls energy expenditure. Curr Opin Lipidol 20: 98-105. crossref(new window)

9.
Wright DC, Han DH, Garcia-Roves PM, Geiger PC, Jones TE, Holloszy JO. 2007. Exercise-induced mitochondrial biogenesis begins before the increase in muscle PGC-$1{\alpha}$ expression. J Biol Chem 282: 194-199. crossref(new window)

10.
Klaus S, Pultz S, Thone-Reineke C, Wolfram S. 2005. Epigallocatechin gallate attenuates diet-induced obesity in mice by decreasing energy absorption and increasing fat oxidation. Int J Obes 29: 615-623. crossref(new window)

11.
Lee MS, Kim CT, Kim IH, Kim Y. 2009. Inhibitory effects of green tea catechin on the lipid accumulation in 3T3-L1 adipocytes. Phytother Res 23: 1088-1091. crossref(new window)

12.
Lee MS, Kim Y. 2009. (-)-Epigallocatechin-3-gallate enhances uncoupling protein 2 gene expression in 3T3-L1 adipocytes. Biosci Biotechnol Biochem 73: 434-436. crossref(new window)

13.
Lee MS, Park JY, Freake H, Kwun IS, Kim Y. 2008. Green tea catechin enhances cholesterol $7{\alpha}$-hydroxylase gene expression in HepG2 cells. Br J Nutr 99: 1182-1185.

14.
Lee MS, Shin Y, Jung S, Kim CT, Kim IH, Kim Y. 2015. Effects of high hydrostatic pressure extract of Korean fresh ginseng on hepatic lipid accumulation and AMPK activation in HepG2 cells. J Food Nutr Res 3: 40-45. crossref(new window)

15.
Rozen S, Skaletsky H. 2000. Primer3 on the WWW for general users and for biologist programmers. Methods Mole Biol 132: 365-386.

16.
Fujiki H, Imai K, Nakachi K, Shimizu M, Moriwaki H, Suganuma M. 2012. Challenging the effectiveness of green tea in primary and tertiary cancer prevention. J Cancer Res Clin Oncol 138: 1259-1270. crossref(new window)

17.
Suganuma M, Okabe S, Oniyama M, Tada Y, Ito H, Fujiki H. 1998. Wide distribution of [$^3H$](-)-epigallocatechin gallate, a cancer preventive tea polyphenol, in mouse tissue. Carcinogenesis 19: 1771-1776. crossref(new window)

18.
Chan CY, Wei L, Castro-Munozledo F, Koo WL. 2011. (-)-Epigallocatechin-3-gallate blocks 3T3-L1 adipose conversion by inhibition of cell proliferation and suppression of adipose phenotype expression. Life Sci 89: 779-785. crossref(new window)

19.
Kim JJY, Tan Y, Xiao L, Sun YL, Qu X. 2013. Green tea polyphenol epigallocatechin-3-gallate enhance glycogen synthesis and inhibit lipogenesis in hepatocytes. BioMed Res Int 2013: 920128.

20.
Yoon JC, Puigserver P, Chen G, Donovan J, Wu Z, Rhee J, Adelmant G, Stafford J, Kahn CR, Granner DK, Newgard CB, Spiegelman BM. 2001. Control of hepatic gluconeogenesis through the transcriptional coactivator PGC-1. Nature 413: 131-138. crossref(new window)

21.
Matravadia S, Martino VB, Sinclair D, Mutch DM, Holloway GP. 2013. Exercise training increases the expression and nuclear localization of mRNA destabilizing proteins in skeletal muscle. Am J Physiol Regul Integr Comp Physiol 305: R822-R831. crossref(new window)

22.
Jun HJ, Joshi Y, Patil Y, Noland RC, Chang JS. 2014. NTPGC-$1{\alpha}$ activation attenuates high-fat diet-induced obesity by enhancing brown fat thermogenesis and adipose tissue oxidative metabolism. Diabetes 63: 3615-3625. crossref(new window)

23.
Valenti D, De Rasmo D, Signorile A, Rossi L, de Bari L, Scala I, Granese B, Papa S, Vacca RA. 2013. Epigallocatechin-3-gallate prevents oxidative phosphorylation deficit and promotes mitochondrial biogenesis in human cells from subjects with Down's syndrome. Biochim Biophys Acta 1832: 542-552. crossref(new window)

24.
Ye Q, Ye L, Xu X, Huang B, Zhang X, Zhu Y, Chen X. 2012. Epigallocatechin-3-gallate suppresses 1-methyl-4-phenylpyridine-induced oxidative stress in PC12 cells via the SIRT1/PGC-$1{\alpha}$ signaling pathway. BMC Complement Altern Med 12: 82. crossref(new window)

25.
Mukherjee S, Siddiqui MA, Dayal S, Ayoub YZ, Malathi K. 2014. Epigallocatechin-3-gallate suppresses proinflammatory cytokines and chemokines induced by Toll-like receptor 9 agonists in prostate cancer cells. J Inflamm Res 7: 89-101.