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Green Tea (-)-Epigallotocatechin-3-Gallate Induces PGC-1α Gene Expression in HepG2 Cells and 3T3-L1 Adipocytes
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  • Journal title : Preventive Nutrition and Food Science
  • Volume 21, Issue 1,  2016, pp.62-67
  • Publisher : The Korean Society of Food Science and Nutrition
  • DOI : 10.3746/pnf.2016.21.1.62
 Title & Authors
Green Tea (-)-Epigallotocatechin-3-Gallate Induces PGC-1α Gene Expression in HepG2 Cells and 3T3-L1 Adipocytes
Lee, Mak-Soon; Lee, Seohyun; Doo, Miae; Kim, Yangha;
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Green tea (Camellia sinensis) is one of the most popular beverages in the world and has been acknowledged for centuries as having significant health benefits. (-)-Epigallocatechin-3-gallate (EGCG) is the most abundant catechin in green tea, and it has been reported to have health benefit effects. Peroxisome proliferator-activated receptor coactivator is a crucial regulator of mitochondrial biogenesis and hepatic gluconeogenesis. The objective of this study was to investigate whether EGCG from green tea can affect the ability of transcriptional regulation on mRNA expression in HepG2 cells and 3T3-L1 adipocytes. To study the molecular mechanism that allows EGCG to control expression, the promoter activity levels of were examined. The mRNA level was measured using quantitative real-time PCR. The -970/+412 bp of promoter was subcloned into the pGL3-Basic vector that includes luciferase as a reporter gene. EGCG was found to up-regulate the mRNA levels significantly with of EGCG in HepG2 cells and differentiated 3T3-L1 adipocytes. promoter activity was also increased by treatment with of EGCG in both cells. These results suggest that EGCG may induce gene expression, potentially through promoter activation.
EGCG;;promoter activity;HepG2 cells;3T3-L1 adipocytes;
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