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SENSITIVE DETERMINATION OF ELEVEN PHENOLIC ENDOCRINE-DISRUPTING CHEMICALS IN HUMAN URINE USING GAS CHROMATOGRAPHY/MASS SPECTROMETRY -SELECTED ION MONITORING
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  • Journal title : Environmental Engineering Research
  • Volume 12, Issue 3,  2007, pp.93-100
  • Publisher : Korean Society of Environmental Engineering
  • DOI : 10.4491/eer.2007.12.3.093
 Title & Authors
SENSITIVE DETERMINATION OF ELEVEN PHENOLIC ENDOCRINE-DISRUPTING CHEMICALS IN HUMAN URINE USING GAS CHROMATOGRAPHY/MASS SPECTROMETRY -SELECTED ION MONITORING
Kim, Hyub; Jang, Cheol-Hyeon;
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 Abstract
I improved an analytical method for determining trace amounts of eleven phenolic endocrine-disrupting chemicals (11 phenolic EOCs) in human urine. The 11 phenolic EOCs were subjected to hydrolysis and then to solid phase extraction with a XAD-4 column. Alkylphenols, chlorophenols, and bisphenol A in XAD-4 column were eluted with acetonitrile, and the eluate was concentrated under a nitrogen stream, and then tert-butyldimethylsilylation. Separation and determination were done by gas chromatography, using mass spectrometry operating in the selective ion monitoring mode for quantitation. For tert-butyldimethylsily (TBDMS) derivatization the recoveries were , the limits of quantitation (LOQ) for the 11 phenolic EOCs in the nanogram-per-milliliter range () were thus achieved by using 1 mL of urine, and the SIM responses were linear with the correlation coefficient varying by . Based on the results for urine samples from unexposed individuals, 4-tert-octylphenol and pentachlorophenol were detected in hydrolysed urine sample. Other alkylphenols, chlorophenols and bisphenol A were not detected.
 Keywords
Endocrine disrupting chemicals;TBDMS;GC/MS-SIM;LOQ;Phenol;
 Language
English
 Cited by
1.
Effects of Korean red ginseng (Panax Ginseng Meyer) on bisphenol A exposure and gynecologic complaints: single blind, randomized clinical trial of efficacy and safety, BMC Complementary and Alternative Medicine, 2014, 14, 1  crossref(new windwow)
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