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Measuring Phosphatase Activity in Peatland Soils: Recent Methodological Advances
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  • Journal title : Environmental Engineering Research
  • Volume 13, Issue 4,  2008, pp.165-168
  • Publisher : Korean Society of Environmental Engineering
  • DOI : 10.4491/eer.2008.13.4.165
 Title & Authors
Measuring Phosphatase Activity in Peatland Soils: Recent Methodological Advances
Freeman, Chris; Jang, In-Young; Zho, Kyoung-Duk; Kang, Ho-Jeong;
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Measurements of phosphatase activity in peatlands are made difficult by the low levels of activity and the characteristically high concentrations of dissolved organic matter within the sediments. These materials may cause high background absorbances in colorimetric assays, and quenching interference in fluorimetric assays. This review describes the development of a new approach which allows such problems to be overcome by using HPLC to separate the interferences from the products of enzymic hydrolysis. This approach is applicable to various environmental samples such as peat, wetland sediment, and sludge which may contain a large amount of interfering organic matters.
Methylumbelliferyl phosphate;HPLC;Quench correction;Organic soil;
 Cited by
Innovative methods in soil phosphorus research: A review, Journal of Plant Nutrition and Soil Science, 2015, 178, 1, 43  crossref(new windwow)
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