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Measuring Phosphatase Activity in Peatland Soils: Recent Methodological Advances
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  • Journal title : Environmental Engineering Research
  • Volume 13, Issue 4,  2008, pp.165-168
  • Publisher : Korean Society of Environmental Engineering
  • DOI : 10.4491/eer.2008.13.4.165
 Title & Authors
Measuring Phosphatase Activity in Peatland Soils: Recent Methodological Advances
Freeman, Chris; Jang, In-Young; Zho, Kyoung-Duk; Kang, Ho-Jeong;
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Measurements of phosphatase activity in peatlands are made difficult by the low levels of activity and the characteristically high concentrations of dissolved organic matter within the sediments. These materials may cause high background absorbances in colorimetric assays, and quenching interference in fluorimetric assays. This review describes the development of a new approach which allows such problems to be overcome by using HPLC to separate the interferences from the products of enzymic hydrolysis. This approach is applicable to various environmental samples such as peat, wetland sediment, and sludge which may contain a large amount of interfering organic matters.
Methylumbelliferyl phosphate;HPLC;Quench correction;Organic soil;
 Cited by
Speir, T. W. and Ross, D. J., Soil phosphatase and sulphatase. In: Burns, R. G. (Ed.), Soil Enzymes. Academic Press, New York, pp. 197-250 (1978)

Parker, F. W., "Soil phosphorus studies: III Plant growth and absorption of phosphorus from culture solutions of different phosphate concentrations," Soil Science, 24, 129-146 (1927) crossref(new window)

Rogers, H. T., "Dephosphorylation of organic phosphorus compounds by soil catalysts," Journal of Soil Science, 54, 439-444 (1942) crossref(new window)

Kroll, L., Kramer, M., and Lorincz, E., "The application of enzyme analysis with phenylphosphate to soils and fertilizers," Agrokem. Talajt, 4, 173-182 (1955)

Ramirez-Martinez, J. R. and McLaren, A. D., "Determination of soil phosphatase activity, Enzymologia, 30, 243-253 (1966)

Skujins, J., Braal, L., and Mclaren, A. D., "Characterization of phosphatase in a terrestrial soil sterilised with an electron beam," Enzymologia, 25, 125-133 (1962)

Tabatabai, M. A. and Bremner, J. M., "Use of p-nitophenyl phosphate for assay of soil phosphatase activity," Soil Biology and Biochemistry, 1, 301-307 (1969) crossref(new window)

Skujins, J., History of abiotic soil enzyme research. In: Burns, R. G. (Ed.), Soil Enzymes. Academic Press, New York, pp. 1-50 (1978)

Tabatabai, M. A., Soil Enzymes. In: Page, A. L. (Ed.), Methods of Soil Analysis; Part 2, Chemical and Microbiological properties, 2nd ed., American Society of Agronomy, Soil Science Society of America. Madison, WI, pp. 903-947, (1982)

Kuster, E., The Microbiology of Peat. In: Heathwaite, A. L. and Gottlich, K. (Eds), Mires: Process, Exploitation and Conservation, J. Wiley, Chichester, UK, pp. 311-324 (1993)

Wright, A. L., Reddy, K. R., Newman, S., and McCormick, P., Extracellular enzyme activity in the soil of the Florida Everglades. In: Report No. 17; Abstracts of the Fifth Symposium on Wetland Biogeochemistry. Royal Holloway Institute for Environmental Research, University of London UK, pp. 144-145, (1997)

Somville, M., "Measurement and study of substrate specificity of exoglucosidase activity in eutrophic water," Applied and Environmental Microbiololgy, 48, 1181-1185 (1984)

Chrost, R. J. and Krambeck, H. J., "Fluorescence correction for measurements of enzyme activity in natural waters using methylumbelliferyl - substrates," Archives of hydrobiology, 106, 79-90 (1986)

King, G. M., "Characterization of -Glucosidase activity in intertidal marine sediments," Applied and Environmental Microbiololgy, 51, 373-380 (1986)

Munster, U., Einio, P., and Nurminen, J., "Evaluation of the measurements of extracellular enzyme activities in a polyhumic lake by means of studies with 4- methylumbelliferyl substrates," Archives of hydrobiology, 115, 321-337 (1989)

Marxsen, J. and Witzel, K. P., "Measurement of exoenzymatic activity in streambed sediments using methylumbelliferyl-substrates," Arch. Hydrobiol. Beih. Ergebn. Limnol, 34, 21-28 (1990)

Marxsen, J. and Witzel, K. P., Significance of Extracellular Enzymes for Organic Matter Degradation and Nutrient Regeneration in Small Streams. In: Chrost, R. J. (Ed), Microbial Enzymes in Aquatic Environments, Springer, New York (1991)

Sinsabaugh, R. L., Gollady, S. W., and Linkins, A. E., "Comparison of epilithic and epixylic biofilm development in a boreal river," Freshwater Biology, 25, 179-187 (1991) crossref(new window)

Boschker, H. T. S. and Cappenberg, T. E., "A sensitive method using 4-methylumbelliferyl-cellobiose as a substrate to measure (1,4) -Glucanase activity in sediments," Applied and Environmental Microbiololgy, 60, 3592-3596 (1994)

Freeman, C., Liska, G., Ostle, N., Jones, S. E., and Lock, M. A., "The use of fluorogenic substrates for measuring enzyme activity in peatlands," Plant and Soil, 175, 147-152 (1995) crossref(new window)

Freeman, C., Lock, M. A., Marxsen, J., and Jones, S. E., "Inhibitory effects of high molecular weight dissolved organic matter upon metabolic processes in biofilms from contrasting rivers and streams," Freshwater Biology, 24, 159-166 (1990) crossref(new window)

Freeman, C., "Using HPLC to eliminate quench interference in fluorogenic substrate assays of microbial enzyme activity," Soil Biology and Biochemistry, 29, 203-205 (1997) crossref(new window)

Kang, H. and Freeman, C., "Measurement of phosphomonoesterase activity in wetland sediments - using HPLC and UV detection," Archives of hydrobiology, 140, 411-417 (1997)

Freeman, C. and Nevison, G. B., "Simultaneous Analysis of Multiple Enzymes in Environmental Samples Using Methylumbelliferyl Substrates and HPLC," Journal of environmental quality, 28, 1378-1380 (1999)