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Speculation on the Identity of Bacteria Named TFOs Occurring in the Inefficient P-Removal Phase of a Biological Phosphorus Removal System
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  • Journal title : Environmental Engineering Research
  • Volume 15, Issue 1,  2010, pp.3-7
  • Publisher : Korean Society of Environmental Engineering
  • DOI : 10.4491/eer.2010.15.1.003
 Title & Authors
Speculation on the Identity of Bacteria Named TFOs Occurring in the Inefficient P-Removal Phase of a Biological Phosphorus Removal System
Lee, Young-Ok; Ahn, Chang-Hoon; Park, Jae-Kwang;
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 Abstract
To better understand the ecology of tetrade forming organisms (TFOs) floating in a large amount of dairy wastewater treatment plant (WWTP) effluent (sequencing batch reactor [SBR]) during the inefficient phosphorus (P) removal process of an enhanced biological P removal system, the TFOs from the effluent of a full scale WWTP were separated and attempts made to culture the TFOs in presence/absence of oxygen. The intact TFOs only grew aerobically in the form of unicellular short-rods. Furthermore, to identify the intact TFOs and unicellular short-rods the DNAs of both were extracted, analyzed using their denaturing gradient gel electrophoresis (DGGE)-profiles and then sequenced. The TFOs and unicellular short-rods exhibited the same banding pattern in their DGGE-profiles, and those sequencing data resulted in their identification as Acinetobacter sp. The intact TFOs appeared in clumps and packages of tetrade cells, and were identified as Acinetobacter sp., which are known as strict aerobes and efficient P-removers. The thick layer of extracellular polymeric substance surrounding Acinetobacter sp. may inhibit phosphate uptake, and the cell morphology of TFOs might subsequently be connected with their survival strategy under the anaerobic regime of the SBR system.
 Keywords
Acinetobacter sp.;Denaturing gradient gel electrophoresis;Enhanced biological phosphorus removal;Extracellular polymeric substance;Sequencing batch reactor;Tetrade forming organisms;
 Language
English
 Cited by
 References
1.
Mino T, Van Loosdrecht MCM, Heijnen JJ. Microbiology and biochemistry of the enhanced biological phosphate removal process. Water Res. 1998;32:3193-3207. crossref(new window)

2.
Ahn CH, Park HD, Lee YO, Park JK. Appearance of novel Gbacteria belonging to Acidobacteria in a dairy wastewater treatment plant. Environ. Technol. 2008;29: 497-504. crossref(new window)

3.
Bond PL, Keller J, Blackall LL. Bio-P and non-bio-P bacteria identification by a novel microbial approach. Water Sci.Technol. 1999;39:13-20.

4.
Crocetti GR, Hugenholtz P, Bond PL, et al. Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantitation. Appl. Environ. Microbiol. 2000;66:1175-1182. crossref(new window)

5.
McMahon KD, Dojka MA, Pace NR, Jenkins D, Keasling JD. Polyphosphate kinase from activated sludge performing enhanced biological phosphorus removal. Appl. Environ. Microbiol. 2002;68:4971-4978. crossref(new window)

6.
Cech JS, Hartman P. Competition between polyphosphate and polysaccharide accumulating bacteria in enhanced biological phosphate removal systems. Water Res. 1993;27: 1219-1225. crossref(new window)

7.
Maszenan AM, Seviour RJ, Patel BKC, Wanner J. A fluorescently-labelled r-RNA targeted oligonucleotide probe for the in situ detection of G-bacteria of the genus Amaricoccus in activated sludge. J. Appl. Microbiol. 2000;88:826-835. crossref(new window)

8.
Nielsen AT, Liu WT, Filipe C, Grady L, Molin S, Stahl DA. Identification of a novel group of bacteria in sludge from a deteriorated biological phosphorus removal reactor. Appl. Environ. Microbiol. 1999;65:1251-1258.

9.
Maszenan AM, Seviour RJ, Patel BKC, et al. Three isolates of novel polyphosphate-accumulating Gram-positive cocci, obtained from activated sludge, belong to a new genus, Tetrasphaera gen. nov., and description of two new species, Tetrasphaera japonica sp, nov and Tetrasphaera australiensis sp nov. Int. J. Syst. Evol. Microbiol. 2000;50:593-603. crossref(new window)

10.
Filipe CDM, Daigger GT. Grady CPL. pH as a key factor in the competition between glycogen-accumulating organisms and phosphorus-accumulating organisms. Water Environ. Res. 2001;73:223-232. crossref(new window)

11.
Saitou N, Nei M. The neighbor-joining method-a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 1987;4:406-425.

12.
Manz W, Amann R, Ludwig W, Wagner M, Schleifer KH. Phylogenetic oligodeoxynucleotide probes for the major subclasses of proteobacteria-problems and solutions. Syst. Appl. Microbiol. 1992;15:593-600. crossref(new window)

13.
Madigan MT, Martinko JM, Parker J, Brock TD. Brock biology of microorganisms. 10th ed. Upper Saddle River. NJ: Prentice Hall/Pearson Education; 2003.

14.
Jenkins D, Richard MG, Daigger GT. Manual on the causes and control of activated sludge bulking and foaming. 2nd ed. Boca Raton: Lewis; 1993.

15.
Muyzer G, Ramsing NB. Molecular methods to study the organization of microbial communities. Water Sci. Technol. 1995;32:1-9.

16.
Towner K. The genus acinetobacter. In: Dworkin, M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E, eds. The prokaryotes: a handbook on the biology of bacteria. 3rd ed. vol. 6. proteobacteria: gamma subclass. New York: Springer; 2006. p. 746-758.

17.
Cardenas E, Cole JR, Tiedje JM, Park JH. Microbial community analysis using RDP II (ribosomal database project II): methods, tools and new advances. Environ. Eng. Res. 2009;14:3-9. crossref(new window)

18.
Seviour RJ, Maszenan AM, Soddell JA, et al. Microbiology of the ‘G-bacteria’ in activated sludge. Environ. Microbiol. 2000;2:581-593. crossref(new window)

19.
Whang LM, Park JK. Competition between polyphosphateand glycogen-accumulating organisms in biological phosphorus removal systems - effect of temperature. Water Sci. Technol. 2002;46:191-194.

20.
Erdal UG, Erdal ZK, Randall CW. The competition between PAOs (phosphorus accumulating organisms) and GAOs (glycogen accumulating organisms) in EBPR (enhanced biological phosphorus removal) systems at different temperatures and the effects on system performance. Water Sci. Technol. 2003;47:1-8.