Publisher : Korean Society of Environmental Engineering
DOI : 10.4491/eer.2010.15.2.057
Title & Authors
Effect of Mixing Methods on the Biodegradation of Sorbed Naphthalene and Phenanthrene in Soils Kim, Hae-Young; Moon, Deok Hyun; Chung, Seon-Yong; Park, Jeong-Hun;
The purpose of this study was to investigate the effect of mixing methods on the biodegradation of sorbed naphthalene and phenanthrene in soils. Biodegradation was initiated by inoculating Pseudomonas sp. KM1 into equilibrated soil slurry vials. Four different mixing methods, including no mixing, orbital shaking, rolling and rotating were utilized to enhance the biodegradation of both naphthalene and phenanthrene. The experimental results showed that the sorbed compounds were more effectively biodegraded with rolling and rotating mixing methods. The sorbed naphthalene concentrations were reduced to 0 mg/kg via the rolling and rotating methods. However, with no mixing and the orbital shaking methods, the sorbed naphthalene concentrations were comparatively high, ranging from 2.59 to 20.45 mg/kg. Similar trends were observed for the biodegradation of phenanthrene, but the concentrations remaining were higher than those of naphthalene, due to the limited bioavailability of the sorbed phenanthrene. The rolling and rotating mixing methods are suggested can distribute bacteria uniformly in the slurry system; improve the mass transfer rate and the probability of physical contact between bacteria and the sorbed contaminants, resulting in higher bioavailability of the contaminants.
Enhanced Heavy Metal Sorption by Surface-Oxidized Activated Carbon Does Not Affect the PAH Sequestration in Sediments, Water, Air, & Soil Pollution, 2012, 223, 6, 3195
Degradation of the long-resistant pharmaceutical compounds carbamazepine and diatrizoate using mixed microbial culture, Journal of Environmental Science and Health, Part A, 2016, 51, 6, 467
Bamforth SM, Singleton I. Bioremediation of polycyclic aromatic hydrocarbons: Current knowledge and future directions. J. Chem. Technol. Biotechnol. 2005;80:723-736.
Huesemann MH, Hausmann TS, Fortman TJ. Does bioavail ability limit biodegradation? A comparison of hydrocarbon biodegradation and desorption rates in aged soils. Biodegradation 2004;15:261-274.
Zhang WX, Bouwer EJ. Biodegradation of benzene, toluene and naphthalene in soil-water slurry microcosms. Biodegradation 1997;8:167-175.
Al-Bashir B, Hawari J, Samson R, Leduc R. Behavior of nitrogen-substituted naphthalenes in flooded soil - Part II. Effect of bioavailability on biodegration kinetics. Water Res. 1994;28:1827-1833.
Huesemann MH, Hausmann TS, Fortman TJ. Assessment of bioavailability limitations during slurry biodegradation of petroleum hydrocarbons in aged soils. Environ. Toxicol. Chem. 2003;22:2853-2860.
Carmichael LM, Christman RF, Pfaender FK. Desorption and mineralization kinetics of phenanthrene and chrysene in contaminated soils. Environ. Sci. Technol. 1997;31:126-132.
Saponaro S, Bonomo L, Petruzzelli G, Romele L, Barbafieri M. Polycyclic aromatic hydrocarbons (PAHS) slurry phase bioremediation of a manufacturing gas plant (MGP) site aged soil. Water, Air, Soil Pollut. 2002;135:219-236.
Fanget B, Devos O, Naffrechoux E. Pyrene transfer from clay particles to water: The role of humic acid. Rev. Sci. Eau 2002;15:95-108.
Hwang S, Min KS, Cutright TJ. PAH biodegradation in soil-water suspensions contaminated with waste oil. Environ. Eng. Res. 2004;9:1-12.
Chiou CT, McGroddy SE, Kile DE. Partition characteristics of polycyclic aromatic hydrocarbons on soils and sediments. Environ. Sci. Technol. 1998;32:264-269.
Oleszczuk P, Baran S. Leaching of individual PAHs in soil varies with the amounts of sewage sludge applied and total organic carbon content. Polish J. Environ. Stud. 2005;14:491-500.
Risk Reduction Engineering Laboratory. Pilot scale demonstration of a slurry phase biological reactor for creosote-contaminated soil-application analysis report. Cincinnati, OH: US Environmental Protection Agency. 1993. Report No.: EPA/540/A5-91/009.
Lewis RF. SITE demonstration of slurry phase biodegradation of PAH contaminated soil. J. Air Waste Manage. Assoc. 1993;43:503-508.
Lauch RP, Herrmann JG, Mahaffey WR, Jones AB, Dosani M, Hessling J. Removal of creosote from soil by bioslurry reactors. Environ. Prog. 1992;11:265-271.
Stinson MK, Skovronek HS, Ellis WD. EPA site demonstration of the BioTrol soil washing process. J. Air Waste Manage. Assoc. 1992;42:96-103.
Woodhull PM, Jerger DE. Temperature effects on kinetics and economics of slurry phasebiological treatment. In: Hinchee, RE, Brockman FJ, Vogel CM, eds. Microbial processes for bioremediation. Columbus: Battelle Press; 1995. p. 289-295.