Advanced SearchSearch Tips
Membrane Biofouling of Seawater Reverse Osmosis Initiated by Sporogenic Bacillus Strain
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Environmental Engineering Research
  • Volume 15, Issue 3,  2010, pp.141-147
  • Publisher : Korean Society of Environmental Engineering
  • DOI : 10.4491/eer.2010.15.3.141
 Title & Authors
Membrane Biofouling of Seawater Reverse Osmosis Initiated by Sporogenic Bacillus Strain
Lee, Jin-Wook; Ren, Xianghao; Yu, Hye-Weon; Kim, Sung-Jo; Kim, In-S.;
  PDF(new window)
The objective of this study was to assess the biofouling characteristics of the Bacillus biofilm formed on reverse osmosis (RO) membranes. For the study, a sporogenic Bacillus sp. was isolated from the seawater intake to a RO process, with two distinct sets of experiments performed to grow the Bacillus biofilm on the RO membrane using a lab-scale crossflow membrane test unit. Two operational feds were used, 9 L sterile-filtered seawater and 109 Bacillus cells, with flow rates of 1 L/min, and a constant 800 psi-pressure and pH 7.6. From the results, the membrane with more fouling, in which the observed permeate flux decreased to 33% of its initial value, showed about 10 and 100 times greater extracellular polymeric substances and spoOA genes expressions, respectively, than the those of the less fouled membrane (flux declined to 20% of its initial value). Interestingly; however, the number of culturable Bacillus sp. in the more fouled membrane was about 10 times less than that of the less fouled membrane. This indicated that while the number of Bacillus had less relevance with respect to the extent of biofouling, the activation of the genes of interest, which is initiative of biofilm development, had a more positive effect on biofouling than the mass of an individual Bacillus bacterium.
Bacillus sp.;Biofilm;Gene expression;Extracellular polymeric substance;Reverse osmosis membrane;spoOA;
 Cited by
Optimized Synthesis Conditions of Polyethersulfone Support Layer for Enhanced Water Flux for Thin Film Composite Membrane,Son, Moon;Choi, Hyeongyu;Liu, Lei;Park, Hosik;Choi, Heechul;

Environmental Engineering Research, 2014. vol.19. 4, pp.339-344 crossref(new window)
Polydopamine coating effects on ultrafiltration membrane to enhance power density and mitigate biofouling of ultrafiltration microbial fuel cells (UF-MFCs), Water Research, 2014, 54, 62  crossref(new windwow)
Fouling and cleaning of reverse osmosis membrane applied to membrane bioreactor effluent treating textile wastewater, Environmental Engineering Research, 2016, 21, 1, 45  crossref(new windwow)
Analysis of raw and pre-treated seawater for potential biofouling precursors, Desalination, 2015, 373, 71  crossref(new windwow)
Surface modification of reverse osmosis membranes with zwitterionic coatings: A potential strategy for control of biofouling, Surface and Coatings Technology, 2015, 279, 171  crossref(new windwow)
Alginate fouling reduction of functionalized carbon nanotube blended cellulose acetate membrane in forward osmosis, Chemosphere, 2015, 136, 204  crossref(new windwow)
The impact of diatoms on the biofouling of seawater reverse osmosis membranes in a model cross-flow system, Desalination, 2016, 392, 8  crossref(new windwow)
Anode direct contact for enhancing power generation and biofouling reduction in ultrafiltration microbial fuel cells, Journal of Chemical Technology & Biotechnology, 2014, 89, 11, 1767  crossref(new windwow)
Optimized Synthesis Conditions of Polyethersulfone Support Layer for Enhanced Water Flux for Thin Film Composite Membrane, Environmental Engineering Research, 2014, 19, 4, 339  crossref(new windwow)
Source water quality shaping different fouling scenarios in a full-scale desalination plant at the Red Sea, Water Research, 2013, 47, 2, 558  crossref(new windwow)
Al-Ahmad M, Abdul Aleem FA, Mutiri A, Ubaisy A. Biofuoling in RO membrane systems Part 1: Fundamentals and control. Desalination 2000;132:173-179. crossref(new window)

Paul DH. Reverse osmosis: scaling, fouling and chemical attack. Desal. Water Reuse 1991;1:8-11.

Khedr MG. Membrane fouling problems in reverse osmosis desalination applications. Desal. Water Reuse 2000;10:8-17.

Jang N, Shon H, Ren X, Vigneswaran S, Kim IS. Characteristics of bio-foulants in the membrane bioreactor. Desalination 2006;200:201-202. crossref(new window)

Ridgway HF, Kelly A, Justice C, Olson BH. Microbial fouling of reverse-osmosis membranes used in advanced wastewater treatment technology: Chemical, bacteriological, and ultrastructural analyses. Appl. Environ. Microbiol. 1983;45:1066-1084.

Baker JS, Dudley LY. Biofouling in membrane systems-a review. Desalination 1998;118:81-90. crossref(new window)

Sadr Ghayeni SB, Beatson PJ, Schneider RP, Fane AG. Adhesion of waste water bacteria to reverse osmosis membranes. J. Membr. Sci. 1998;138:29-42. crossref(new window)

Flemming HC, Schaule G. Biofouling on membranes-A microbiological approach. Desalination 1988;70:95-119.

Bereschenko LA, Heilig GHJ, Nederlof MM, Van Loosdrecht MCM, Stams AJM, Euverink GJW. Molecular characterization of the bacterial communities in the different compartments of a full-scale reverse-osmosis water purification plant. Appl. Environ. Microbiol. 2008;74:5297-5304. crossref(new window)

Chen CL, Liu WT, Chong ML, et al. Community structure of microbial biofilms associated with membrane-based water purification processes as revealed using a polyphasic approach. Appl. Microbiol. Biotechnol. 2004;63:466-473. crossref(new window)

Ivnitsky H, Katz I, Minz D, et al. Bacterial community composition and structure of biofilms developing on nanofiltration membranes applied to wastewater treatment. Water Res. 2007;41:3924-3935. crossref(new window)

Lee J, Kim SM, Jung JY, Oh BS, Kim IS, Hong SK. Analysis of total bacteria, enteric members of $\gamma$-proteobacteria and microbial communities in seawater as indirect indicators of quantifying biofouling. Environ. Eng. Res. 2009;14:19-25. crossref(new window)

Jung JY, Lee J, Kim S, Kim IS. Characteristics on the adhesion and the high pressure resistance of bacteria in biofilm of SWRO process. J. of KSEE 2009;31:51-57.

Hamon MA, Lazazzera BA. The sporulation transcription factor Spo0A is required for biofilm development in Bacillus subtilis. Mol. Microbiol. 2001;42:1199-1209.

Kim S, Cho D, Lee MS, Oh BS, Kim JH, Kim IS. SEAHERO R&D program and key strategies for the scale-up of a seawater reverse osmosis (SWRO) system. Desalination 2009;238:1-9. crossref(new window)

Jochem FJ. Morphology and DNA content of bacterioplankton in the northern Gulf of Mexico: Analysis by epifluorescence microscopy and flow cytometry. Aquat. Microb. Ecol. 2001;25:179-194. crossref(new window)

Herzberg M, Elimelech M. Physiology and genetic traits of reverse osmosis membrane biofilms: A case study with Pseudomonas aeruginosa. ISME Journal 2008;2:180-194. crossref(new window)

Bin Z, Baosheng S, Min J, Taishi G, Zhenghong G. Extraction and analysis of extracellular polymeric substances in membrane fouling in submerged MBR. Desalination 2008;227:286-294. crossref(new window)

Brill JA, Wiegel J. Differentiation between spore-forming and asporogenic bacteria using a PCR and Southern hybridization based method. J. Microbiol. Methods 1997;31:29-36. crossref(new window)

Sutherland IW. Biofilm exopolysaccharides: a strong and sticky framework. Microbiology 2001;147:3-9.

Schneider RP, Ferreira LM, Binder P, et al. Dynamics of organic carbon and of bacterial populations in a conventional pretreatment train of a reverse osmosis unit experiencing severe biofouling. J. Membr. Sci. 2005;266:18-29. crossref(new window)

Hu JY, Song LF, Ong SL, Phua ET, Ng WJ. Biofiltration pretreatment for reverse osmosis (RO) membrane in a water reclamation system. Chemosphere 2005;59:127-133. crossref(new window)

Branda SS, Chu F, Kearns DB, Losick R, Kolter R. A major protein component of the Bacillus subtilis biofilm matrix. Mol. Microbiol. 2006;59:1229-1238. crossref(new window)

Flemming HC. Reverse osmosis membrane biofouling. Exp. Therm Fluid Sci. 1997;14:382-391. crossref(new window)

Beloin C, Ghigo JM. Finding gene-expression patterns in bacterial biofilms. Trends Microbiol. 2005;13:16-19. crossref(new window)

Lawrence JR, Swerhone GDW, Leppard GG, et al. Scanning transmission X-ray, laser scanning, and transmission electron microscopy mapping of the exopolymeric matrix of microbial biofilms. Appl. Environ. Microbiol. 2003;69:5543-5554. crossref(new window)