Advanced SearchSearch Tips
Abundances of triclosan-degrading microorganisms in activated sludge systems
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Environmental Engineering Research
  • Volume 20, Issue 1,  2015, pp.105-109
  • Publisher : Korean Society of Environmental Engineering
  • DOI : 10.4491/eer.2014.074
 Title & Authors
Abundances of triclosan-degrading microorganisms in activated sludge systems
Lee, Do Gyun; Chu, Kung-Hui;
  PDF(new window)
Triclosan is a synthetic antimicrobial agent used in numerous industrial and personal care products. Triclosan collected in wastewater treatment plants can be biodegraded up to 80%. However, little is studied about the abundances of known triclosan-degrading bacteria in activated sludge systems. A previous study reported that Sphingopyxis strain KCY1 isolated from activate sludge can cometabolically degrade triclosan. Recently, a quantitative PCR (qPCR) assay specific to strain KCY1 has been developed. Thus, this study investigated the abundance of strain KCY1 in three different activated sludge wastewater treatments using a qPCR assay. Additionally, ammonia-oxidizing bacteria (AOB), known as triclosan-degraders, and amoA gene were quantified. Strain KCY1 were detected in activated sludge samples from three different wastewater treatment plants. The concentrations of strain KCY1 and AOB were on the order of gene copies/mL, while amoA gene concentration was on the order of gene copies/mL.
Activated sludge;Ammonia-oxidizing Bacteria triclosan;Quantitative PCR;Triclosan-degrading bacteria;
 Cited by
Triclosan in water, implications for human and environmental health, SpringerPlus, 2016, 5, 1  crossref(new windwow)
Sabaliunas D, Webb SF, Hauk A, Jacob M, Eckhoff WS. Environmental fate of triclosan in the River Aire Basin, UK. Water Res. 2003;37:3145-3154. crossref(new window)

Schweizer HP. Triclosan: a widely used biocide and its link to antibiotics. FEMS Microbiol. Lett. 2001;202:1-7. crossref(new window)

Latch DE, Packer JL, Arnold WA, McNeill K. Photochemical conversion of triclosan to 2,8-dichlorodibenzo-p-dioxin in aqueous solution. J. Photochem. Photobiol. A Chem. 2003;158: 63-66. crossref(new window)

Hundt K, Martin D, Hammer E, Jonas U, Kindermann MK, Schauer F. Transformation of triclosan by Trametes versicolor and Pycnoporus cinnabarinus. Appl. Environ. Microbiol. 2000;66:4157-4160. crossref(new window)

Foran CM, Bennett ER, Benson WH. Developmental evaluation of a potential non-steroidal estrogen: triclosan. Mar. Environ. Res. 2000;50:153-156. crossref(new window)

Braoudaki M, Hilton AC. Low level of cross-resistance between triclosan and antibiotics in Escherichia coli K-12 and E. coli O55 compared to E.coli O157. FEMS Microbiol. Lett. 2004;235: 305-309. crossref(new window)

Chen X, Casas ME, Nielsen JL, Wimmer R, Bester K. Identification of triclosan-o-sulfate and other transformation products of triclosan formed by activated sludge. Sci. Total Environ. 2015;505:39-46. crossref(new window)

McAvoy DC, Schatowitz B, Jacob M, Hauk A, Eckhoff WS. Measurement of triclosan in wastewater treatment systems. Environ. Toxicol. Chem. 2002;21:1323-1329. crossref(new window)

Singer H, Mueller S, Tixier C, Pillonel L. Triclosan: occurrence and fate of a widely used biocide in the aquatic environment: field measurements in wastewater treatment plants, surface waters, and lake sediments. Environ. Sci. Technol. 2002;36: 4998-5004. crossref(new window)

Lee DG, Zhao F, Rezenom YH, Russell DH, Chu KH. Biodegradation of triclosan by a wastewater microorganism. Water Res. 2012;46:4226-4234. crossref(new window)

Lee DG, Cho KC, Chu KH. Identification of triclosan-degrading bacteria in a triclosan enrichment culture using stable isotope probing. Biodegradation 2014;25:55-65. crossref(new window)

Lee DG, Chu KH. Effects of growth substrate on triclosan biodegradation potential of oxygenase-expressing bacteria. Chemosphere 2013;93:1904-1911. crossref(new window)

Roh H, Subramanya N, Zhao F, Yu CP, Sandt J, Chu KH. Biodegradation potential of wastewater micropollutants by ammonia- oxidizing bacteria. Chemosphere 2009;77:1084-1089. crossref(new window)

Hay AG, Dees PM, Sayler GS. Growth of a bacterial consortium on triclosan. FEMS Microbiol. Lett. 2001;36:105-112. crossref(new window)

Tastan BE, Donmez G. Biodegradation of pesticide triclosan by A. versicolor in simulated wastewater and semi-synthetic media. Pestic. Biochem. Physiol. 2015;118:33-37. crossref(new window)

Yu CP, Ahuja R, Sayler G, Chu KH. Quantitative molecular assay for fingerprinting microbial communities of wastewater and estrogen-degrading consortia. Appl. Environ. Microbiol. 2005;71:1433-1444. crossref(new window)

Harms G, Layton AC, Dionisi HM, et al. Real-time PCR quantification of nitrifying bacteria in a municipal wastewater treatment plant. Environ. Sci. Technol. 2003;37:343-351. crossref(new window)

Hermansson A, Lindgren PE. Quantification of ammonia-oxidizing bacteria in arable soil by real-time PCR. Appl. Environ. Microbiol. 2001;67:972-976. crossref(new window)

Rotthauwe JH, Witzel KP, Liesack W. The ammonia monooxygenase structural gene amoA as a functional marker: Molecular fine-scale analysis of natural ammonia-oxidizing populations. Appl. Environ. Microbiol. 1997;63:4704-4712.

Hoshino T, Terahara T, Tsuneda S, Hirata A, Inamori Y. Molecular analysis of microbial population transition associated with the start of denitrification in a wastewater treatment process. J. Appl. Microbiol. 2005;99:1165-1175. crossref(new window)

Araki N, Yamaguchi T, Yamazaki S, Harada H. Quantification of amoA gene abundance and their amoA mRNA levels in activated sludge by real-time PCR. Water Sci. Technol. 2004; 50:1-8.

Geets J, De Cooman M, Wittebolle L, et al. Real-time PCR assay for the simultaneous quantification of nitrifying and denitrifying bacteria in activated sludge. Appl. Microbiol. Biotechnol. 2007;75:211-221. crossref(new window)

Kowalchuk GA, Stephen JR. Ammonia-oxidizing bacteria: a model for molecular microbial ecology. Annu. Rev. Microbiol. 2001;55:485-529. crossref(new window)

Painter H, Loveless J. Effect of temperature and pH value on the growth-rate constants of nitrifying bacteria in the activated- sludge process. Water Res. 1983;17:237-248. crossref(new window)

Zhang T, Jin T, Yan Q, et al. Occurrence of ammonia-oxidizing archaea in activated sludges of a laboratory scale reactor and two wastewater treatment plants. J. Appl. Microbiol. 2009;107: 970-977. crossref(new window)

Park HD, Wells GF, Bae H, Criddle CS, Francis CA. Occurrence of ammonia-oxidizing archaea in wastewater treatment plant bioreactors. Appl. Environ. Microbiol. 2006;72:5643-5647. crossref(new window)

Limpiyakorn T, Sonthiphand P, Rongsayamanont C, Polprasert C. Abundance of amoA genes of ammonia-oxidizing archaea and bacteria in activated sludge of full-scale wastewater treatment plants. Bioresour. Technol. 2011;102:3694-3701. crossref(new window)

Roh H, Chu KH. A $17{\beta}$-estradiol-utilizing bacterium, Sphingomonas strain KC8: part I - characterization and abundance in wastewater treatment plants. Environ. Sci. Technol. 2010;44:4943-4950. crossref(new window)