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Monitoring of fecal contamination in a partly restored urban stream in Seoul, Korea
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  • Journal title : Environmental Engineering Research
  • Volume 21, Issue 2,  2016, pp.211-218
  • Publisher : Korean Society of Environmental Engineering
  • DOI : 10.4491/eer.2015.049
 Title & Authors
Monitoring of fecal contamination in a partly restored urban stream in Seoul, Korea
Seo, Eun-Young; Jung, Dawoon; Yong, Seung-Cheon; Park, Rho Young; Lee, Young-Ok; Ahn, Tae-Seok;
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Cheonggye-cheon is a partly restored urban stream located in central Seoul. We monitored fecal contamination using three different fecal indicators, total coliforms (TC), fecal coliforms (FC) and E. coli, to assess differences in each indicator on days of varying weather conditions. Presumptive TC, FC and E. coli colonies were identified by their 16S rRNA sequences. The results showed that enumeration of E. coli provided a better reflection of fecal contamination of the stream than TC and FC. The main sources of contamination were the inflow of fecal-polluted groundwater from the vicinity of a subway line and two inflowing streams. The fecal contamination was worsened on days with heavy rain because untreated sewage from a collecting facility flowed into the stream. Moreover, growth potential of fecal indicator (E. coli) in situ induced by algal exudates was measured. Our results suggest that an appropriate standard based on E. coli rather than TC and FC should be established for improving water quality management strategies of Cheonggye-cheon in the future.
Escherichia coli;Fecal contamination;Microbial water quality;Urban stream;
 Cited by
Cho KK. The history and future of Cheonggye-cheon that flows again. Kor. Soc. Civil Eng. 2005;53:98-110.

Song DY. Spatial morphology and its socio-economic implication in Seoul's Cheonggycheon area. Eco. 2003;4:166-190.

Jang YK. Study on the impact of Cheonggyecheon restoration on property values [dissertation]. Seoul: Univ. of Hongik; 2013.

Seoul Metropolitan facilities Management Corporation. Cheonggeycheon water quality management method and academic investigation. Seoul: Seoul Metropolitan facilities Management Corporation; 2012.

Park Y, Lee H, Kim S, Ko GP. Assessment of fecal pollution and bacterial community structure in restored section of Cheonggyecheon stream. J. Kor. Soc. Water Qual. 2009;25:76-83.

Stumpf CH, Piehler MF, Thompson S, Noble RT. Loading of fecal indicator bacteria in North Carolina tidal creek headwaters: Hydrographic patterns and terrestrial runoff relationships. Wat. Res. 2010;44:4704-4715. crossref(new window)

Nagels J, Davies-Colley R, Donnison A, Muirhead R. Faecal contamination over flood events in a pastoral agricultural stream in New Zealand. Water Sci. Technol. 2002;45:45-52.

Howard I, Espigares E, Lardelli P, Martín JL. Espigares M. Evaluation of microbiological and physicochemical indicators for wastewater treatment. Environ. Toxicol. 2004;19:241-249. crossref(new window)

Cabelli VJ, Dufour AP, McCabe LJ, Levin MA. A marine recreational water quality criterion consistent with indicator concepts and risk analysis. J. Water Pollut. Control Fed. 1983;55:1306-1314.

Edberg S, Rice EW, Karlin RJ, Allen MJ. Escherichia coli: The best biological drinking water indicator for public health protection. J. Appl. Microbiol. 2000;88:106S-116S. crossref(new window)

Fiello M, Mikell AT Jr, Moore MT, Cooper CM. Variability in the characterization of total coliforms, fecal coliforms and Escherichia coli in recreational water supplies of north Mississippi, USA. Bull. Environ. Contam. Toxicol. 2014;93:133-137. crossref(new window)

USEPA. Method 1603: Escherichia coli in water by membrane filtration using modified membrane-Thermotolerant Escherichia coli Agar (Modified m-TEC). Pennsylvania: U.S. Environmental Protection Agency; 2006.

Lee YO. Comparative studies on detection of E. coli in surface water. Kor. J. Limnol. 1996;29:313-321.

Park J, Ahn T, Lee H, Lee Y. Comparison of total and faecal coliforms as faecal indicator in eutrophicated surface water. Water Sci. Technol. 2006;54:185-190.

McDonald A, Kay D. Enteric bacterial concentrations in reservoir feeder streams: Baseflow characteristics and response to hydrograph events. Water Res. 1981;15:961-968. crossref(new window)

Byappanahalli MN, Shively DA, Nevers MB, Sadowsky MJ, Whitman RL. Growth and survival of Escherichia coli and Enterococci populations in the macro-alga Cladophora (Chlorophyta). FEMS Microbiol. Ecol. 2003;46:203-211. crossref(new window)

Kacar A, Gungor F. Comparison of fecal coliform bacteria before and after wastewater treatment plant in the Izmir Bay (Eastern Aegean Sea). Environ. Monit. Assess. 2010;162:355-363. crossref(new window)

Little JL, Saffran KA, Fent L. Land use and water quality relationships in the lower little bow river watershed, Alberta, Canada. Water Qual. Res. J. Can. 2003;38:563-584.

Cho KH, Cha SM, Kang J-H, et al. Meteorological effects on the levels of fecal indicator bacteria in an urban stream: A modeling approach. Water Res. 2010;44:2189-2202. crossref(new window)

Field KG, Samadpour M. Fecal source tracking, the indicator paradigm, and managing water quality. Water Res. 2007;41: 3517-3538. crossref(new window)

Power ML, Littlefield-Wyer J, Gordon DM, Veal DA, Slade MB. Phenotypic and genotypic characterization of encapsulated Escherichia coli isolated from blooms in two Australian lakes. Environ. Microbiol. 2005;7:631-640. crossref(new window)

Kavka G. Zur Frage der Nachweisbarkeit und Uberlebenszeit von Kolibakterien in verschiedenen Biotopen [dissertation]. Vienna: Univ. of Vienna; 1978.

Anderson KL, Whitlock JE, Harwood VJ. Persistence and differential survival of fecal indicator bacteria in subtropical waters and sediments. Appl. Environ. Microbiol. 2005;71:3041-3048. crossref(new window)

Fujioka R, Sian-Denton C, Borja M, Castro J, Morphew K. Soil: The environmental source of Escherichia coli and Enterococci in Guam's streams. J. Appl. Microbiol. 1998;85:83S-89S. crossref(new window)

Solo-Gabriele HM, Wolfert MA, Desmarais TR, Palmer CJ. Sources of Escherichia coli in a coastal subtropical environment. Appl. Environ. Microbiol. 2000;66:230-237. crossref(new window)

Ksoll WB, Ishii S, Sadowsky MJ, Hicks RE. Presence and sources of fecal coliform bacteria in epilithic periphyton communities of Lake Superior. Appl. Environ. Microbiol. 2007;73:3771-3778. crossref(new window)

Badgley BD, Thomas FIM, Harwood VJ. Quantifying environmental reservoirs of fecal indicator bacteria associated with sediment and submerged aquatic vegetation. Environ. Microbiol. 2011;13:932-942. crossref(new window)

Davies CM, Long JA, Donald M, Ashbolt NJ. Survival of fecal microorganisms in marine and freshwater sediments. Appl. Environ. Microbiol. 1995;61:1888-1896.

Byappanahalli MN, Fujioka RS. Evidence that tropical soil environment can support the growth of Escherichia coli. Water Sci. Technol. 1998;38:171-174.

Harwood VJ, Butler J, Parrish D, Wagner V. Isolation of fecal coliform bacteria from the diamondback terrapin (Malaclemys terrapin centrata). Appl. Environ. Microbiol. 1999;65:865-867.

Desmarais TR, Solo-Gabriele HM, Palmer CJ. Influence of soil on fecal indicator organisms in a tidally influenced subtropical environment. Appl. Environ. Microbiol. 2002;68:1165-1172. crossref(new window)

Dixit SM, Gordon DM, Wu X-Y, Chapman T, Kailasapathy K, Chin JJC. Diversity analysis of commensal porcine Escherichia coli-associations between genotypes and habitat in the porcine gastrointestinal tract. Microbiol. 2004;150:1735-1740. crossref(new window)

Kim JW, Pachepsky YA, Shelton DR, Coppock C. Effect of streambed bacterial release on E. coli concentration: Monitoring and modeling with the modified SWAT. Ecol. Model. 2010;221:1592-1604. crossref(new window)

Pachepsky YA, Shelton DR. Escherichia coli and fecal coliforms in freshwater and estuarine sediments. Crit. Rev. Env. Sci. Technol. 2011;41:1067-1110. crossref(new window)

Lavoie MC. Identification of strains isolated as total and fecal coliforms and comparison of both groups as indicators of fecal pollution in tropical climates. Can. J. Microbiol. 1983;29:689-693. crossref(new window)

ISO (International standard). Water quality-Detection and enumeration of coliform organisms, thermotolerant coliforms organisms and presumptive E. coli Part 2 Multiple tube method. 9308-2-1990. Geneva: ISO; 1990.

Fiello M, Mikell AT Jr, Moore MT, Cooper CM. Variability in the characterization of total coliforms, fecal coliforms and Escherichia coli in recreational water supplies of north Mississippi, USA. Bull. Environ. Contam. Toxicol. 2014;93:133-137. crossref(new window)

Odonkor ST, Ampofo JK. Escherichia coli as an indicator of bacteriological quality of water: an overview. Microbiol. Res. 2013;4:5-11. crossref(new window)

Noble RT, Moore DF, Leecaster MK, McGee CD, Weisberg SB. Comparison of total coliform, fecal coliform, and enterococcus bacterial indicator response for ocean recreational water quality testing. Water Res. 2003;37:1637-1643. crossref(new window)

Chao WL. Evaluation of colilert-18 for the detection of coliforms and Escherichia coli in tropical fresh water. Lett. Appl. Microbiol. 2006;42:115-120. crossref(new window)

Fricker EJ, Illingworth KS, Fricker CR. Use of two formulations of Colilert and quantitray for assessment of the bacteriological quality of water. Water Res. 1997;31:2495-2499. crossref(new window)

Eckner KF. Comparison of membrane filtration and multiple-tube fermentation by the Colilert and Enterolert methods for detection of waterborne coliform bacteria, Escherichia coli, and Enterococci used in drinking and bathing water quality monitoring in southern Sweden. Appl. Environ. Microbiol. 1998;64:3079-3083.

Pisciotta JM, Rath DF, Stanek PA, Flanery DM, Harwood VJ. Marine bacteria cause false-positive results in the Colilert-18 rapid identification test for Escherichia coli in Florida waters. Appl. Environ. Microbiol. 2002;68:539-544. crossref(new window)

Rice EW, Allen MJ, Edberg SC. Efficacy of beta-glucuronidase assay for identification of Escherichia coli by the defined-substrate technology. Appl. Environ. Microbiol. 1990;56:1203-1205.