Advanced SearchSearch Tips
Antimicrobial, Antioxidant and Hemolytic Activity of Water-soluble Extract of Mottled Anemone Urticina crassicornis
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Fisheries and aquatic sciences
  • Volume 18, Issue 4,  2015, pp.341-347
  • Publisher : The Korean Society of Fisheries and Aquatic Science
  • DOI : 10.5657/FAS.2015.0341
 Title & Authors
Antimicrobial, Antioxidant and Hemolytic Activity of Water-soluble Extract of Mottled Anemone Urticina crassicornis
Lee, Ye Jin; Kim, Chan-Hee; Oh, Hye Young; Go, Hye-Jin; Park, Nam Gyu;
  PDF(new window)
We evaluated the biological activities of five water extracts of tissue of the mottled anemone Urticina crassicornis. Most extracts exhibited broad-spectrum antimicrobial activity as determined by ultrasensitive radial diffusion assay (URDA) against gram-positive and -negative bacteria, including a fish pathogen, Aeromonas hydrophila, but no activity against fungi. The activity of the extracts was abolished by tryptic digestion, indicating that protein compounds were responsible for the antimicrobial activity. Furthermore, in a 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging activity assay, only the visceral tissue extract showed activity. However, no extract had hemolytic activity against human red blood cells. Consequently, this study suggests the water-soluble extract of mottled anemone to be a promising source of proteinaceous antimicrobial compounds that can be utilized for development of novel antibiotics.
Urticina crassicornis;Water-soluble extract;Antimicrobial activity;Antioxidant activity;Hemolysis;
 Cited by
Alhazmi MI. 2015. Isolation of Aeromonas spp. from Food Products: Emerging Aeromonas Infections and Their Significance in Public Health. J AOAC Int 98, 927-929. crossref(new window)

Alma MH, Mavi A, Yildirim A, Digrak M. and Hirata T. 2003. Screening chemical composition and in vitro antioxidant and antimicrobial activities of the essential oils from Origanum syriacum L. growing in Turkey. Biol Pharm Bull 26, 1725-1729. crossref(new window)

Aneiros A. and Garateix A. 2004. Bioactive peptides from marine sources: pharmacological properties and isolation procedures. J Chromatogr B Analyt Technol Biomed Life Sci 803, 41-53. crossref(new window)

Bhakuni DS and Rawat DS. 2006. Bioactive marine natural products. Springer Science & Business Media, NY, US.

Bradshaw J. 2003. Cationic antimicrobial peptides: issues for potential clinical use. BioDrugs 17, 233-240. crossref(new window)

Brown KL and Hancock RE. 2006. Cationic host defense antimicrobial. peptides. Curr Opin Immunol 18, 24-30. crossref(new window)

Diaz Lopez FJ and Montalvo C. 2015. A comprehensive review of the evolving and cumulative nature of eco-innovation in the chemical industry. J Clean Prod 102, 30-43. crossref(new window)

Dantas G, Sommer MO, Oluwasegun RD and Church GM. 2008. Bacteria subsisting on antibiotics. Science 320, 100-103. crossref(new window)

de Lima LA, Migliolo L, Barreiro e Castro C, Pires Dde O, Lopez-Abarrategui C, Goncalves EF, Vasconcelos IM, de Oliveira JT, Otero-Gonzalez Ade J, Franco OL and Dias SC. 2013. Identification of a novel antimicrobial peptide from Brazilian coast coral Phyllogorgia dilatata. Protein Pept Lett 20, 1153-1158. crossref(new window)

Derby CD. 2007. Escape by inking and secreting: marine molluscs avoid predators through a rich array of chemicals and mechanisms. Biol Bull 213, 274-289. crossref(new window)

Fellenberg M, Coksezen A and Meyer B. 2010. Characterization of picomole amounts of oligosaccharides from glycoproteins by 1H NMR spectroscopy. Angew Chem Int Ed Engl 49, 2630-2633. crossref(new window)

Gross L, Mohn F, Moll N, Meyer G, Ebel R, Abdel-Mageed WM and Jaspars M. 2010. Organic structure determination using atomic-resolution scanning probe microscopy. Nat Chem 2, 821-825. crossref(new window)

Grundmann H, Aires-de-Sousa M, Boyce J and Tiemersma E. 2006. Emergence and resurgence of meticillin-resistant Staphylococcus aureus as a public-health threat. The Lancet 368, 874-885. crossref(new window)

Hagiwara K, Garcia Hernandez JE, Harper MK, Carroll A, Motti CA, Awaya J, Nguyen HY and Wright AD. 2015. Puupehenol, a potent antioxidant antimicrobial meroterpenoid from a Hawaiian deep-water Dactylospongia sp. sponge. J Nat Prod 78, 325-329. crossref(new window)

Izadpanah A and Gallo RL. 2005. Antimicrobial peptides. J Am Acad Dermatol 52, 381-390; quiz 391-382. crossref(new window)

Jacobs L and Chenia HY. 2007. Characterization of integrons and tetracycline resistance determinants in Aeromonas spp. isolated from South African aquaculture systems. Int J Food Microbiol 114, 295-306. crossref(new window)

Juvvadi P, Vunnam S and Merrifield RB. 1996. Synthetic Melittin, Its Enantio, Retro, and Retroenantio Isomers, and Selected Chimeric Analogs: Their antibacterial, hemolytic, and lipid bilayer action. J Am Chem Soc 118, 8989-8997. crossref(new window)

Kahl R and Kappus H. 1993. Toxicology of the synthetic antioxidants BHA and BHT in comparison with the natural antioxidant vitamin E. Z Lebensm Unters Forsch 196, 329-338. crossref(new window)

Keymanesh K, Soltani S and Sardari S. 2009. Application of antimicrobial peptides in agriculture and food industry. World J Microbiol Biotechnol 25, 933-944. crossref(new window)

Leal MC, Puga J, Serodio J, Gomes NC and Calado R. 2012. Trends in the discovery of new marine natural products from invertebrates over the last two decades--where and what are we bioprospecting? PLoS One 7, e30580. crossref(new window)

Livingstone DR. 2001. Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms. Mar Poll Bull 42, 656-666. crossref(new window)

Lushchak VI. 2011. Environmentally induced oxidative stress in aquatic animals. Aquat Toxicol 101, 13-30. crossref(new window)

Mayer AM, Rodriguez AD, Taglialatela-Scafati O and Fusetani N. 2013. Marine pharmacology in 2009-2011: marine compounds with antibacterial, antidiabetic, antifungal, anti-inflammatory, antiprotozoal, antituberculosis, and antiviral activities; affecting the immune and nervous systems, and other miscellaneous mechanisms of action. Mar Drugs 11, 2510-2573. crossref(new window)

Mehbub MF, Lei J, Franco C and Zhang W. 2014. Marine sponge derived natural products between 2001 and 2010: trends and opportunities for discovery of bioactives. Mar Drugs 12, 4539-4577. crossref(new window)

Mohan KV, Rao SS and Atreya CD. 2010. Antiviral activity of selected antimicrobial peptides against vaccinia virus. Antiviral Res 86, 306-311. crossref(new window)

Noga EJ, Ullal AJ, Corrales J and Fernandes JM. 2011. Application of antimicrobial polypeptide host defenses to aquaculture: Exploitation of downregulation and upregulation responses. Comp Biochem Physiol Part D Genomics Proteomics 6, 44-54. crossref(new window)

Ovchinnikova TV, Balandin SV, Aleshina GM, Tagaev AA, Leonova Y F, Krasnodembsky E D, Men'shenin AV and Kokryakov VN. 2006. Aurelin, a novel antimicrobial peptide from jellyfish Aurelia aurita with structural features of defensins and channel-blocking toxins. Biochem Biophys Res Commun 348, 514-523. crossref(new window)

Park NG, Silphaduang U, Moon HS, Seo JK, Corrales J and Noga EJ. 2011. Structure-activity relationships of piscidin 4, a piscine antimicrobial peptide. Biochemistry 50, 3288-3299. crossref(new window)

Pietrzyk AJ, Bujacz A, Mak P, Potempa B and Niedziela T. 2015. Structural studies of Helix aspersa agglutinin complexed with GalNAc: A lectin that serves as a diagnostic tool. Int J Biol Macromol 81, 1059-1068. crossref(new window)

Rocha J, Calado R and Leal M. 2015. Marine Bioactive Compounds from Cnidarians. In: Springer Handbook of Marine Biotechnology, Kim SK, ed. pp. 823-849. Springer Berlin Heidelberg.

Rocha J, Peixe L, Gomes NC and Calado R. 2011. Cnidarians as a source of new marine bioactive compounds--an overview of the last decade and future steps for bioprospecting. Mar Drugs 9, 1860-1886. crossref(new window)

Ruiz J, Calderon J, Rondon-Villarreal P and Torres R. 2014. Analysis of structure and hemolytic activity relationships of antimicrobial peptides (AMPs). In: Advances in Computational Biology, Castillo LF et. al, eds., Vol. 232, pp. 253-258. Springer International Publishing.

Seo JK, Lee MJ, Go HJ, Kim YJ and Park NG. 2014. Antimicrobial function of the GAPDH-related antimicrobial peptide in the skin of skipjack tuna, Katsuwonus pelamis. Fish Shellfish Immunol 36, 571-581. crossref(new window)

Song JI and Cha HR. 2002. Taxonomy of Actiniidae (Anthozoa, Actiniaria, Thenaria, Endomvaria) from Korea. Korean J Syst Zool 18, 253-270.

Subramanian B, Sangappellai T, Rajak RC and Diraviam B. 2011. Pharmacological and biomedical properties of sea anemones Paracondactylis indicus, Paracondactylis sinensis, Heteractis magnifica and Stichodactyla haddoni from East coast of India. Asian Pac J Trop Med 4, 722-726. crossref(new window)

Suleria HA, Osborne S, Masci P and Gobe G. 2015. Marine-Based Nutraceuticals: An innovative trend in the food and supplement industries. Mar Drugs 13, 6336-6351. crossref(new window)

Thangaraj S, Bragadeeswaran S, Suganthi K and Kumaran NS. 2011. Antimicrobial properties of sea anemone Stichodactyla mertensii and Stichodactyla gigantea from Mandapam coast of India. Asian Pac J Trop Biomed 1, S43-S46. crossref(new window)

Vizioli J and Salzet M. 2002. Antimicrobial peptides versus parasitic infections? Trends Parasitol 18, 475-476. crossref(new window)