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Potential Association between Insulin-like Growth Factor-1 Receptor Activity and Surf Clam Spisula sachalinensis Larvae Survival
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  • Journal title : Fisheries and aquatic sciences
  • Volume 18, Issue 4,  2015, pp.417-420
  • Publisher : The Korean Society of Fisheries and Aquatic Science
  • DOI : 10.5657/FAS.2015.0417
 Title & Authors
Potential Association between Insulin-like Growth Factor-1 Receptor Activity and Surf Clam Spisula sachalinensis Larvae Survival
Choi, Youn Hee; Nam, Taek Jeong;
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We investigated the relationship between viability and IGF-1 receptor (IGF-1R) activity in D-shaped and umbo larvae of the surf clam Spisula sachalinensis after treatment with vitrification solution (VS) or freezing. In a toxicity assay, VS1, containing 5 M dimethyl sulfoxide (DMSO), was very harmful to D-shaped and umbo larvae. However, VS2, containing 5 M ethylene glycol (EG), was not harmful to either larval stage. Although VS2 had a promising toxicity test outcome, none of the larvae survived vitrification. After immersion into VSs and freezing, IGF-1R -subunits were detected in all larvae; however, tyrosine phosphorylation of intracellular -subunits was detected only in the control and live groups. These results suggest that activation of IGF-1R may influence surf clam larvae viability.
Spisula sachalinensis;Surf clam;D-shaped larvae;Umbo larvae;Vitrification;IGF-1R;
 Cited by
Adams TE, Epa VC, Garrett TPJ and Ward CW. 2000. Structure and function of the type 1 insulin-like growth factor receptor. Cell Mol Life Sci 57, 1050-1093. crossref(new window)

Chao NH, Lin TT, Chen YJ, Hsu HW and Liao IC. 1997. Cryopreservation of late embryos and early larvae in the oyster and hard clam. Aquaculture 155, 31-44. crossref(new window)

Chen SL and Tian YS. 2005. Cryopreservation of flounder (Paralichthys olivaceus) embryos by vitrification. Theriogenology 63, 1207-1219., crossref(new window)

Choi YH, Nam TJ and Kuwano K. 2013. Cryopreservation of gametophytic thalli of Porphyra yezoensis (Rhodophyceae) by one-step fast cooling. J Appl Phycol 25, 531-535. crossref(new window)

Codina M, de la serrana DG, Sanchez-Gurmaches J, Montserrat N, Chistyakova O, Navarro I and Gutierrez J. 2008. Metabolic and mitogenic effects of IGF-II in rainbow trout (Oncorhynchus mykiss) myocytes in culture and the role of IGF-II in the PI3K/Akt and MAPK signalling pathways. Gen Comp Endocrinol 157, 116-124. crossref(new window)

Cuevas-Uribe R, Leibo SP, Daly J and Tiersch TR. 2011. Production of channel catfish with sperm cryopreserved by rapid non-equilibrium cooling. Cryobiology 63, 186-197. crossref(new window)

Czech MP. 1989. Signal transmission by the insulin-like growth factors. Cell 59, 235-238. crossref(new window)

Duan C. 1997. The insulin-like growth factor system and its biological actions in fish. Ame Zool 37, 491-503. crossref(new window)

Duan C and Xu Q. 2005. Roles of insuli-like growth factor (IGF) binding proteins in regulating IGF actions. Gen Comp Endocrinol 142, 44-52. crossref(new window)

Gwo JC 1995. Cryopreservation of oyster (Crassostrea gigas) embryos. Theriogenology 43, 1163-1174. crossref(new window)

Hagedorn M and Kleinhans FW. 2011. Problems and prospects in cryopreservation of fish embryos, in: T.R. Tiersch, C.C. Green (Eds.), Cryopreservation in Aquatic Species, second ed., World Aquaculture Society, Baton Rouge, Louisiana, USA, 483-502.

Paredes E, Bellas J and Adams S. 2013. Comparative cryopreservation study of trochophore larvae from two species of bivalves: Pacific oyster (Crassostrea gigas) and blue mussel (Mytilus galloprovincialis). Cryobiology 67, 274-279. crossref(new window)

Robles V, Cabrita E, de Paz P, Cunado, Anel L and Herraez MP. 2004. Effect of a vitrification protocol on the lactate dehydrogenase and glucose-6-phosphate dehydrogenase activities and the hatching rates of zebrafish (Danio rerio) and turbot (Scophthalmus maximus) embryos. Theriogenology 61, 1367-1379. crossref(new window)

Robles V, Cabrita E, Acker JP and Herraez P. 2008. Embryo cryopreservation: what we know until now. CRC Press, Taylor & Francis Group, London.

Tian YS, Chen SL, Yan AS, Ji XS and Yu GC. 2003. Studies on vitrification method of sea perch (Lateolabrax japonicus) embryos. Acta Zool Sinica 49, 843-850.

Zhang T and Rawson DM. 1996. Feasibility studies on vitrification of intact zebrafish (Brachydanio rerio). Cryobiology 33, 1-13. crossref(new window)