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Potential Association between Insulin-like Growth Factor-1 Receptor Activity and Surf Clam Spisula sachalinensis Larvae Survival
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  • Journal title : Fisheries and aquatic sciences
  • Volume 18, Issue 4,  2015, pp.417-420
  • Publisher : The Korean Society of Fisheries and Aquatic Science
  • DOI : 10.5657/FAS.2015.0417
 Title & Authors
Potential Association between Insulin-like Growth Factor-1 Receptor Activity and Surf Clam Spisula sachalinensis Larvae Survival
Choi, Youn Hee; Nam, Taek Jeong;
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 Abstract
We investigated the relationship between viability and IGF-1 receptor (IGF-1R) activity in D-shaped and umbo larvae of the surf clam Spisula sachalinensis after treatment with vitrification solution (VS) or freezing. In a toxicity assay, VS1, containing 5 M dimethyl sulfoxide (DMSO), was very harmful to D-shaped and umbo larvae. However, VS2, containing 5 M ethylene glycol (EG), was not harmful to either larval stage. Although VS2 had a promising toxicity test outcome, none of the larvae survived vitrification. After immersion into VSs and freezing, IGF-1R -subunits were detected in all larvae; however, tyrosine phosphorylation of intracellular -subunits was detected only in the control and live groups. These results suggest that activation of IGF-1R may influence surf clam larvae viability.
 Keywords
Spisula sachalinensis;Surf clam;D-shaped larvae;Umbo larvae;Vitrification;IGF-1R;
 Language
English
 Cited by
 References
1.
Adams TE, Epa VC, Garrett TPJ and Ward CW. 2000. Structure and function of the type 1 insulin-like growth factor receptor. Cell Mol Life Sci 57, 1050-1093. crossref(new window)

2.
Chao NH, Lin TT, Chen YJ, Hsu HW and Liao IC. 1997. Cryopreservation of late embryos and early larvae in the oyster and hard clam. Aquaculture 155, 31-44. crossref(new window)

3.
Chen SL and Tian YS. 2005. Cryopreservation of flounder (Paralichthys olivaceus) embryos by vitrification. Theriogenology 63, 1207-1219., crossref(new window)

4.
Choi YH, Nam TJ and Kuwano K. 2013. Cryopreservation of gametophytic thalli of Porphyra yezoensis (Rhodophyceae) by one-step fast cooling. J Appl Phycol 25, 531-535. crossref(new window)

5.
Codina M, de la serrana DG, Sanchez-Gurmaches J, Montserrat N, Chistyakova O, Navarro I and Gutierrez J. 2008. Metabolic and mitogenic effects of IGF-II in rainbow trout (Oncorhynchus mykiss) myocytes in culture and the role of IGF-II in the PI3K/Akt and MAPK signalling pathways. Gen Comp Endocrinol 157, 116-124. crossref(new window)

6.
Cuevas-Uribe R, Leibo SP, Daly J and Tiersch TR. 2011. Production of channel catfish with sperm cryopreserved by rapid non-equilibrium cooling. Cryobiology 63, 186-197. crossref(new window)

7.
Czech MP. 1989. Signal transmission by the insulin-like growth factors. Cell 59, 235-238. crossref(new window)

8.
Duan C. 1997. The insulin-like growth factor system and its biological actions in fish. Ame Zool 37, 491-503. crossref(new window)

9.
Duan C and Xu Q. 2005. Roles of insuli-like growth factor (IGF) binding proteins in regulating IGF actions. Gen Comp Endocrinol 142, 44-52. crossref(new window)

10.
Gwo JC 1995. Cryopreservation of oyster (Crassostrea gigas) embryos. Theriogenology 43, 1163-1174. crossref(new window)

11.
Hagedorn M and Kleinhans FW. 2011. Problems and prospects in cryopreservation of fish embryos, in: T.R. Tiersch, C.C. Green (Eds.), Cryopreservation in Aquatic Species, second ed., World Aquaculture Society, Baton Rouge, Louisiana, USA, 483-502.

12.
Paredes E, Bellas J and Adams S. 2013. Comparative cryopreservation study of trochophore larvae from two species of bivalves: Pacific oyster (Crassostrea gigas) and blue mussel (Mytilus galloprovincialis). Cryobiology 67, 274-279. crossref(new window)

13.
Robles V, Cabrita E, de Paz P, Cunado, Anel L and Herraez MP. 2004. Effect of a vitrification protocol on the lactate dehydrogenase and glucose-6-phosphate dehydrogenase activities and the hatching rates of zebrafish (Danio rerio) and turbot (Scophthalmus maximus) embryos. Theriogenology 61, 1367-1379. crossref(new window)

14.
Robles V, Cabrita E, Acker JP and Herraez P. 2008. Embryo cryopreservation: what we know until now. CRC Press, Taylor & Francis Group, London.

15.
Tian YS, Chen SL, Yan AS, Ji XS and Yu GC. 2003. Studies on vitrification method of sea perch (Lateolabrax japonicus) embryos. Acta Zool Sinica 49, 843-850.

16.
Zhang T and Rawson DM. 1996. Feasibility studies on vitrification of intact zebrafish (Brachydanio rerio). Cryobiology 33, 1-13. crossref(new window)