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Screening and classification of mulberry silkworm, Bombyx mori based on thermotolerance
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 Title & Authors
Screening and classification of mulberry silkworm, Bombyx mori based on thermotolerance
Chandrakanth, Nalavadi; Moorthy, Shunmugam M.; Ponnuvel, Kangayam M.; Sivaprasad, Vankadara;
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 Abstract
The tropical climate prevailing in India adversely affects temperate bivoltine silkworm rearing and causes crop loss especially during summer. Identification of high temperature tolerant bivoltine breeds by screening for thermotolerance in the silkworm, Bombyx mori (Lepidoptera: Bombycidae) is an essential prerequisite for the development of thermotolerant bivoltine breeds / hybrids. Therefore, in this study, 20 silkworm breeds were reared at different temperatures (25 ± 1℃,32 ± 1℃, 34 ± 1℃ and 36 ± 1℃) for 6 h every day from 3rd d of 5th instar to till spinning. Significant differences (p < 0.01) were found among all the rearing traits over temperature. Based on pupation percentage, SK4C and BHR3 were identified as thermotolerant bivoltine breeds. Hierarchical clustering analysis based on rearing traits at tested temperatures grouped 20 silkworm breeds in four clusters which included one cluster each of susceptible and tolerant, and two clusters of moderately tolerant silkworm breeds. This suggests that clustering based on rearing data at high temperatures by using Euclidean distance can be an effective approach in classifying the silkworm breeds on their thermotolerance capacity. The identified breeds would be used for development of thermo tolerant bivoltine silkworm breeds / hybrids.
 Keywords
thermotolerance;rearing traits;pupation percentage;euclidean distance;
 Language
English
 Cited by
 References
1.
Allard RB, Bradshaw AO (1964) Implications of genotype environment interaction in applied plant breeding. Crop Science 4, 503-506. crossref(new window)

2.
Begum AN, Basavaraja HK, Rekha M, Ahsan MM, Datta RK (2001) Breeding resource material for the evolution of thermo-tolerant breeds. Int J Ind Entomol 2 (2), 111-117.

3.
Benchamin KV, Jolly MS (1986) Principles of silkworm rearing; in: Proceedings of seminar on problems and prospects of sericulture. Mahalingam S (eds.), pp. 63-106, Vellore, India.

4.
Bindroo BB, Moorthy SM (2014) Genetic divergence, implication of diversity, and conservation of silkworm, Bombyx mori. International Journal of Biodiversity Article ID 564850, 15 pages.

5.
Chatterjee SN, Datta RK (1992) Hierarchical clustering of 54 races and strain of mulberry silkworm, Bombyx mori: Significance of biochemical parameters. Theor Applications Genet 85, 394-402.

6.
Chavadi VB, Aparna HS, Manjunatha HB (2006) Impact of heat shock on heat shock proteins expression, biological and commercial traits of Bombyx mori. Insect Science 13, 243-250. crossref(new window)

7.
Datta RK, Basavaraja HK, Mal Reddy N, Nirmal Kumar S, Suresh Kumar N, Ramesh Babu, Ahsan M, Jayaswal KP (2001) Breeding of new productive bivoltine hybrid, CSR12 x CSR6 of silkworm, Bombyx mori L. Int J Ind Entomol 3, 127-133.

8.
Feder ME (1996) Ecological and evolutionary physiology of stress proteins and the stress response: the Drosophila melanogaster model; in: Animals and temperature: Phenotypic and evolutionary adaptation to temperature. Johnston IA, Bennett AF (eds.), pp. 79-102 Cambridge University Press.

9.
Harlan JR (1971) Agricultural origins: centres and non-centers. Science 174, 468-474. crossref(new window)

10.
Kato M, Nagayasu K, Ninagi O, Hara W, Watanabe A (1989) Studies on resistance of the silkworm, Bombyx mori L. for high temperature. Proceedings of the 6th International Congress of SABRAO (II), India, 953-956.

11.
Krishnaswami S, Narasimhanna MN, Surayanarayana SK, Kumararaj S (1973) Manual on sericulture - Silkworm rearing, UN Food and Agriculture Organisation, Rome.

12.
Kumar NS, Basavaraja HK, Joge PG, Mal Reddy N, Kalpana GV, Dandin SB (2006) Development of new robust bivoltine hybrid (CSR46 x CSR47) of Bombyx mori L for tropics. Indian J Seric 45(1), 21-29.

13.
Kumar NS, Basavaraja HK, Kishor Kumar CM, Mal Reddy N, Datta RK (2002) On the breeding of CSR18 x CSR19 a robust hybrid of silkworm, Bombyx mori L for tropics. Int J Indus Entomol 5(2), 153-162.

14.
Kumar NS, Lakshmi H, Saha AK, Bindroo BB, Longkumer N (2012) Evaluation of bivoltine silkworm breeds of Bombyx mori L. under West Bengal conditions. Universal Journal of Environmental Research and Technology 2(5), 393-401.

15.
Kumar NS, Yamamoto T, Basavaraja HK, Datta RK (2001) Studies on the effect of high temperature on F1 hybrids between polyvoltines and bivoltine silkworm races of Bombyx mori L. Int J Ind Entomol 2, 123-127.

16.
Kumaresan P, Sinha RK, Raje Urs S (2007) An analysis of genetic variation and divergence in Indian tropical polyvoltine silkworm (Bombyx mori L.) genotypes. Caspian J Env Sci 5 (1), 11-17.

17.
Kumari SS, Subbarao SV, Misra S, Murty US (2011) Screening strains of the mulberry silkworm, Bombyx mori, for thermotolerance. J of Insect Science 11, 116. crossref(new window)

18.
Lakshmi H, Chandrashekharaiah, Ramesh Babu M, Raju PJ, Saha AK, Bajpai AK (2011) HTO5 x HTP5, The new bivoltine silkworm (Bombyx mori L.) hybrid with thermo-tolerance for tropical areas. International Journal of Plant, Animal and Environmental Sciences 1(2), 88-104.

19.
Mohammadis A, Prasanna BM (2003). Analysis of genetic diversity in crop plants. Crop Science 43, 1235-1248. crossref(new window)

20.
Moorthy SM, Das SK, Kar NB, Mandal K, Bajpai AK (2007) Breeding of bivoltine silkworm breeds suitable for tropics and identification of multi x bi silkworm hybrid for commercial exploitation in Eastern India. In perspectives of Cytology and Genetics 13, 215-227.

21.
Nezhad SM, Mirhosseini SZ, Gharahveysi S, Mavvajpour M, Seidavi AR, Naserani M (2010) Genetic diversity and classification of 51 strains of silkworm Bombyx mori (Lepidoptera: Bombycidae) germplasm based on larval phenotypic data using Ward’s and UPGMA methods. African Journal of Biotechnology 9(39), 6594-6600.

22.
Pal NB, Moorthy SM (2011) Assessment of variability in larval and cocoon traits in some genotypes of bivoltine silkworm, Bombyx mori L. International Journal of Research in Biological Sciences 1(4), 59-65.

23.
Pillai VS, Krishnaswami S (1980) Effect of high temperature on the survival rate, cocoon quality and fecundity of Bombyx mori L. In: Sericulture Symposium and Seminar, pp. 141-148, Tamil Nadu Agriculture University, Coimbatore, India.

24.
Rahmathulla VK (2012) Management of climatic factors for successful silkworm (Bombyx mori L.) crop and higher silk production: A review. Psyche 2012, Article ID 121234, 12 pages.

25.
Ramesha C, Anuradha CM, Lakshmi H, Sugnana Kumari S, Seshagiri SV, Goel AK, Suresh Kumar C (2010) Nutrigenetic traits analysis for identification of nutritionally efficient silkworm germplasm breeds. Biotechnology 9, 131-140. crossref(new window)

26.
Ramesha C, Seshagiri SV, Rao CGP (2009) Evaluation and identification of superior polyvoltine crossbreeds of mulberry silkworm, Bombyx mori L. Journal of Entomology 6, 179-188. crossref(new window)

27.
Shirota T (1992) Selection of healthy silkworm strain through high temperature rearing of fifth instar larvae. Reports of the Silk Science Research Institute 40, 33-40.

28.
Spagnoletti Zeuli PL, Qualset CO (1987) Geographic diversity for quantitative spike characters in a world collection of durum wheat. Crop Science 27, 235-241. crossref(new window)

29.
Tazima Y, Ohnuma A (1995) Preliminary experiments on the breeding procedure for synthesizing a high temperature resistant commercial strain of the silkworm, Bombyx mori L. Silk Science Research Institute Japan 43, 1-16.

30.
Thangavelu K, Sinha RK, Mahadevamurthy TS, Radha Krishnan S, Kumaresan P, Mohan B, Rayaradder FR, Sekar S (2000) Catalogue on silkworm (Bombyx mori L.) germplasm, CSGRC, Hosur, India.

31.
Vijayan K, Nair CV, Raje Urs S (2010) Assessment of genetic diversity in the tropical mulberry Silkworm (Bombyx mori L.) with mtDNA-SSCP and SSR marker. Emir J Food Agric 22 (2), 71-83. crossref(new window)

32.
Zhang YH, Xu AY, Wei YD, Li MW, Hou CX, Zhang GZ (2002) Studies on feeding habits of silkworm germplasm resources for artificial diet without mulberry. Acta Sericologica Sinica 28, 333-336.