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Mangiferin inhibits tumor necrosis factor-α-induced matrix metalloproteinase-9 expression and cellular invasion by suppressing nuclear factor-κB activity
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  • Journal title : BMB Reports
  • Volume 48, Issue 10,  2015, pp.559-564
  • Publisher : Korean Society for Biochemistry and Molecular Biology
  • DOI : 10.5483/BMBRep.2015.48.10.003
 Title & Authors
Mangiferin inhibits tumor necrosis factor-α-induced matrix metalloproteinase-9 expression and cellular invasion by suppressing nuclear factor-κB activity
Dilshara, Matharage Gayani; Kang, Chang-Hee; Choi, Yung Hyun; Kim, Gi-Young;
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We investigated the effects of mangiferin on the expression and activity of metalloproteinase (MMP)-9 and the invasion of tumor necrosis factor (TNF)--stimulated human LNCaP prostate carcinoma cells. Reverse-transcription polymerase chain reaction (RT-PCR) and western blot analysis showed that mangiferin significantly reversed TNF--induced mRNA and protein expression of MMP-9 expression. Zymography data confirmed that stimulation of cells with TNF- significantly increased MMP-9 activity. However, mangiferin substantially reduced the TNF--induced activity of MMP-9. Additionally, a matrigel invasion assay showed that mangiferin significantly reduced TNF--induced invasion of LNCaP cells. Compared to untreated controls, TNF--stimulated LNCaP cells showed a significant increase in nuclear factor- (NF-) luciferase activity. However, mangiferin treatment markedly decreased TNF--induced NF- luciferase activity. Furthermore, mangiferin suppressed nuclear translocation of the NF- subunits p65 and p50. Collectively, our results indicate that mangiferin is a potential anti-invasive agent that acts by suppressing NF--mediated MMP-9 expression.
Invasion;Mangiferin;Matrix metalloproteinase-9;Nuclear factor kappa B;
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