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Knockdown of microtubule actin crosslinking factor 1 inhibits cell proliferation in MC3T3-E1 osteoblastic cells
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  • Journal title : BMB Reports
  • Volume 48, Issue 10,  2015, pp.583-588
  • Publisher : Korean Society for Biochemistry and Molecular Biology
  • DOI : 10.5483/BMBRep.2015.48.10.098
 Title & Authors
Knockdown of microtubule actin crosslinking factor 1 inhibits cell proliferation in MC3T3-E1 osteoblastic cells
Hu, Lifang; Su, Peihong; Li, Runzhi; Yan, Kun; Chen, Zhihao; Shang, Peng; Qian, Airong;
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Microtubule actin crosslinking factor 1 (MACF1), a widely expressed cytoskeletal linker, plays important roles in various cells by regulating cytoskeleton dynamics. However, its role in osteoblastic cells is not well understood. Based on our previous findings that the association of MACF1 with F-actin and microtubules in osteoblast-like cells was altered under magnetic force conditions, here, by adopting a stable MACF1-knockdown MC3T3-E1 osteoblastic cell line, we found that MACF1 knockdown induced large cells with a binuclear/multinuclear structure. Further, immunofluorescence staining showed disorganization of F-actin and microtubules in MACF1-knockdown cells. Cell counting revealed significant decrease of cell proliferation and cell cycle analysis showed an S phase cell cycle arrest in MACF1-knockdown cells. Moreover and interestingly, MACF1 knockdown showed a potential effect on cellular MTT reduction activity and mitochondrial content, suggesting an impact on cellular metabolic activity. These results together indicate an important role of MACF1 in regulating osteoblastic cell morphology and function.
Cell morphology;Cell proliferation;Cytoskeleton;MACF1;Osteoblastic cell;
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Fuchs E and Karakesisoglou I (2001) Bridging cytoskeletal intersections. Genes Dev 15, 1-14 crossref(new window)

Kodama A, Karakesisoglou I, Wong E, Vaezi A and Fuchs E (2003) ACF7: an essential integrator of microtubule dynamics. Cell 115, 343-354 crossref(new window)

Leung CL, Green KJ and Liem RK (2002) Plakins: a family of versatile cytolinker proteins. Trends Cell Biol 12, 37-45 crossref(new window)

Leung CL, Sun D, Zheng M, Knowles DR and Liem RK (1999) Microtubule actin cross-linking factor (MACF): a hybrid of dystonin and dystrophin that can interact with the actin and microtubule cytoskeletons. J Cell Biol 147, 1275-1286 crossref(new window)

Sun D, Leung CL and Liem RK (2001) Characterization of the microtubule binding domain of microtubule actin crosslinking factor (MACF): identification of a novel group of microtubule associated proteins. J Cell Sci 114, 161-172

Karakesisoglou I, Yang Y and Fuchs E (2000) An epidermal plakin that integrates actin and microtubule networks at cellular junctions. J Cell Biol 149, 195-208 crossref(new window)

Bernier G, Pool M, Kilcup M, Alfoldi J, De Repentigny Y and Kothary R (2000) Acf7 (MACF) is an actin and microtubule linker protein whose expression predominates in neural, muscle, and lung development. Dev Dyn 219, 216-225 crossref(new window)

Wu X, Kodama A and Fuchs E (2008) ACF7 regulates cytoskeletal-focal adhesion dynamics and migration and has ATPase activity. Cell 135, 137-148 crossref(new window)

Wu X, Shen QT, Oristian DS et al (2011) Skin stem cells orchestrate directional migration by regulating microtubule-ACF7 connections through GSK3beta. Cell 144, 341-352 crossref(new window)

Sanchez-Soriano N, Travis M, Dajas-Bailador F, Goncalves-Pimentel C, Whitmarsh AJ and Prokop A (2009) Mouse ACF7 and drosophila short stop modulate filopodia formation and microtubule organisation during neuronal growth. J Cell Sci 122, 2534-2542 crossref(new window)

Antonellis PJ, Pollock LM, Chou SW et al (2014) ACF7 is a hair-bundle antecedent, positioned to integrate cuticular plate actin and somatic tubulin. J Neurosci 34, 305-312 crossref(new window)

Qian AR, Hu LF, Gao X et al (2009) Large gradient high magnetic field affects the association of MACF1 with actin and microtubule cytoskeleton. Bioelectromagnetics 30, 545-555 crossref(new window)

Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65, 55-63 crossref(new window)

Suzuki R, Matsuno S, Sakagami H, Okada Y and Shirataki Y (2014) Search of new cytotoxic crude materials against human oral squamous cell carcinoma using 1H NMRbased metabolomics. Anticancer Res 34, 4117-4120

Al-Rubeai M, Chalder S, Bird R and Emery AN (1991) Cell cycle, cell size and mitochondrial activity of hybridoma cells during batch cultivation. Cytotechnology 7, 179-186 crossref(new window)

Pagliacci MC, Spinozzi F, Migliorati G et al (1993) Genistein inhibits tumour cell growth in vitro but enhances mitochondrial reduction of tetrazolium salts: a further pitfall in the use of the MTT assay for evaluating cell growth and survival. Eur J Cancer 29A, 1573-1577 crossref(new window)

Kitami T, Logan DJ, Negri J et al (2012) A chemical screen probing the relationship between mitochondrial content and cell size. PLoS One 7, e33755 crossref(new window)

Ka M, Jung EM, Mueller U and Kim WY (2014) MACF1 regulates the migration of pyramidal neurons via microtubule dynamics and GSK-3 signaling. Dev Biol 395, 4-18 crossref(new window)

Moulding DA, Blundell MP, Spiller DG et al (2007) Unregulated actin polymerization by WASp causes defects of mitosis and cytokinesis in X-linked neutropenia. J Exp Med 204, 2213-2224 crossref(new window)

Hossain MM, Hwang DY, Huang QQ, Sasaki Y and Jin JP (2003) Developmentally regulated expression of calponin isoforms and the effect of h2-calponin on cell proliferation. Am J Physiol Cell Physiol 284, C156-167 crossref(new window)

Zhu J, Beattie EC, Yang Y, Wang HJ, Seo JY and Yang LX (2005) Centrosome impairments and consequent cytokinesis defects are possible mechanisms of taxane drugs. Anticancer Res 25, 1919-1925

Menon MB and Gaestel M (2015) Sep(t)arate or not - how some cells take septin-independent routes through cytokinesis. J Cell Sci 128, 1877-1886 crossref(new window)

D'Avino PP, Giansanti MG and Petronczki M (2015) Cytokinesis in animal cells. Cold Spring Harb Perspect Biol 7, a015834 crossref(new window)

Livak KJ and Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT Method. Methods 25, 402-408 crossref(new window)

Qian A, Zhang W, Weng Y et al (2008) Gravitational environment produced by a superconducting magnet affects osteoblast morphology and functions. Acta Astronautica 63, 929-946 crossref(new window)

Mundra JJ, Terskiy A and Howells RD (2012) Naltrindole inhibits human multiple myeloma cell proliferation in vitro and in a murine xenograft model in vivo. J Pharmacol Exp Ther 342, 273-287 crossref(new window)

Louis KS and Siegel AC (2011) Cell viability analysis using trypan blue: manual and automated methods. Methods Mol Biol 740, 7-12 crossref(new window)

Qian AR, Gao X, Zhang W et al (2013) Large Gradient High Magnetic Fields Affect Osteoblast Ultrastructure and Function by Disrupting Collagen I or Fibronectin/alphabeta1 Integrin. PLoS One 8, e51036 crossref(new window)