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Transcriptional regulation of Niemann-Pick C1-like 1 gene by liver receptor homolog-1
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  • Journal title : BMB Reports
  • Volume 48, Issue 9,  2015, pp.513-518
  • Publisher : Korean Society for Biochemistry and Molecular Biology
  • DOI : 10.5483/BMBRep.2015.48.9.253
 Title & Authors
Transcriptional regulation of Niemann-Pick C1-like 1 gene by liver receptor homolog-1
Lee, Eui Sup; Seo, Hyun Jung; BacK, Su Sun; Han, Seung Ho; Jeong, Yeon Ji; Lee, Jin Wook; Choi, Soo Young; Han, Kyuhyung;
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Factors that modulate cholesterol levels have major impacts on cardiovascular disease. Niemann-Pick C1-like 1 (NPC1L1) functions as a sterol transporter mediating intestinal cholesterol absorption and counter-balancing hepatobiliary cholesterol excretion. The liver receptor homolog 1 (LRH-1) had been shown to regulate genes involved in hepatic lipid metabolism and reverse cholesterol transport. To study whether human NPC1L1 gene is regulated transcriptionally by LRH-1, we have analyzed evolutionary conserved regions (ECRs) in HepG2 cells. One ECR was found to be responsive to the LRH-1. Through deletion studies, LRH-1 response element was identified and the binding of LRH-1 was demonstrated by EMSA and ChIP assays. When SREBP2, one of several transcription factors which had been shown to regulate NPC1L1 gene, was co-expressed with LRH-1, synergistic transcriptional activation resulted. In conclusion, we have identified LRH-1 response elements in NPC1L1 gene and propose that LRH-1 and SREBP may play important roles in regulating NPC1L1 gene. [BMB Reports 2015; 48(9): 513-518]
 Cited by
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