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Gecko proteins induce the apoptosis of bladder cancer 5637 cells by inhibiting Akt and activating the intrinsic caspase cascade
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  • Journal title : BMB Reports
  • Volume 48, Issue 9,  2015, pp.531-536
  • Publisher : Korean Society for Biochemistry and Molecular Biology
  • DOI : 10.5483/BMBRep.2015.48.9.117
 Title & Authors
Gecko proteins induce the apoptosis of bladder cancer 5637 cells by inhibiting Akt and activating the intrinsic caspase cascade
Kim, Geun-Young; Park, Soon Yong; Jo, Ara; Kim, Mira; Leem, Sun-Hee; Jun, Woo-Jin; Shim, Sang In; Lee, Sang Chul; Chung, Jin Woong;
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Gecko proteins have long been used as anti-tumor agents in oriental medicine, without any scientific background. Although anti-tumor effects of Gecko proteins on several cancers were recently reported, their effect on bladder cancer has not been investigated. Thus, we explored the anti-tumor effect of Gecko proteins and its cellular mechanisms in human bladder cancer 5637 cells. Gecko proteins significantly reduced the viability of 5637 cells without any cytotoxic effect on normal cells. These proteins increased the Annexin-V staining and the amount of condensed chromatin, demonstrating that the Gecko proteinsinduced cell death was caused by apoptosis. Gecko proteins suppressed Akt activation, and the overexpression of constitutively active form of myristoylated Akt prevented Gecko proteins-induced death of 5637 cells. Furthermore, Gecko proteins activated caspase 9 and caspase 3/7. Taken together, our data demonstrated that Gecko proteins suppressed the Akt pathway and activated the intrinsic caspase pathway, leading to the apoptosis of bladder cancer cells. [BMB Reports 2015; 48(9): 531-536]
Akt;Apoptosis;Bladder cancer;Caspase;Gecko proteins;
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Protective effect and mechanism of action of saponins isolated from the seeds of gac (Momordica cochinchinensis Spreng.) against cisplatin-induced damage in LLC-PK1 kidney cells, Bioorganic & Medicinal Chemistry Letters, 2016, 26, 5, 1466  crossref(new windwow)
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Botteman MF, Pashos CL, Redaelli A, Laskin B and Hauser R (2003) The health economics of bladder cancer: a comprehensive review of the published literature. Pharmacoeconomics 21, 1315-1330 crossref(new window)

Kaplan AL, Litwin MS and Chamie K (2014) The future of bladder cancer care in the USA. Nat Rev Urol 11, 59-62 crossref(new window)

Stenzl A, Cowan NC, De Santis M et al (2009) The updated EAU guidelines on muscle-invasive and metastatic bladder cancer. Eur Urol 55, 815-825 crossref(new window)

Herr HW and Morales A (2008) History of bacillus Calmette-Guerin and bladder cancer: an immunotherapy success story. J Urol 179, 53-56 crossref(new window)

Addeo R, Caraglia M, Bellini S et al (2010) Randomized phase III trial on gemcitabine versus mytomicin in recurrent superficial bladder cancer: evaluation of efficacy and tolerance. J Clin Oncol 28, 543-548 crossref(new window)

Connett GJ and Lee BW (1994) Treating childhood asthma in Singapore: when West meets East. BMJ 308, 1282-1284 crossref(new window)

Liu F, Wang JG, Wang SY, Li Y, Wu YP and Xi SM (2008) Antitumor effect and mechanism of Gecko on human esophageal carcinoma cell lines in vitro and xenografted sarcoma 180 in Kunming mice. World J Gastroenterol 14, 3990-3996 crossref(new window)

Wang YX, Gu XX, Geng D et al (2014) Differentiation of bel-7402 human hepatocarcinoma cells induced by aqueous extracts of fresh gecko (AG) and its anti-tumor activity in vivo. J Ethnopharmacol 155, 1583-1588 crossref(new window)

Song Y, Wang JG, Li RF et al (2012) Gecko crude peptides induce apoptosis in human liver carcinoma cells in vitro and exert antitumor activity in a mouse ascites H22 xenograft model. J Biomed Biotechnol 2012, 743573

Zhang SX, Zhu C, Ba Y et al (2012) Gekko-sulfated glycopeptide inhibits tumor angiogenesis by targeting basic fibroblast growth factor. J Biol Chem 287, 13206-13215 crossref(new window)

Jeong AJ, Chung CN, Kim HJ et al (2012) Gecko Proteins Exert Anti-Tumor Effect against Cervical Cancer Cells Via PI3-Kinase/Akt Pathway. Korean J Physiol Pharmacol 16, 361-365 crossref(new window)

Li J, Jiang K, Qiu X et al (2014) Overexpression of CXCR4 is significantly associated with cisplatin-based chemotherapy resistance and can be a prognostic factor in epithelial ovarian cancer. BMB Rep 47, 33-38 crossref(new window)

Altomare DA and Testa JR (2005) Perturbations of the AKT signaling pathway in human cancer. Oncogene 24, 7455-7464 crossref(new window)

Zhao B and Li X (2014) Altholactone induces reactive oxygen species-mediated apoptosis in bladder cancer T24 cells through mitochondrial dysfunction, MAPK-p38 activation and Akt suppression. Oncol Rep 31, 2769-2775

Choi EM, Kwak SJ, Kim YM et al (2005) COX-2 inhibits anoikis by activation of the PI-3K/Akt pathway in human bladder cancer cells. Exp Mol Med 37, 199-203 crossref(new window)

Sagar S, Esau L, Holtermann K et al (2013) Induction of apoptosis in cancer cell lines by the Red Sea brine pool bacterial extracts. BMC Complement Altern Med 13, 344 crossref(new window)

Lee HW, Jang KS, Choi HJ, Jo A, Cheong JH and Chun KH (2014) Celastrol inhibits gastric cancer growth by induction of apoptosis and autophagy. BMB Rep 47, 697-702 crossref(new window)

Watanabe T, Nakamura S, Ono T et al (2014) Pyrrolidinium fullerene induces apoptosis by activation of procaspase-9 via suppression of Akt in primary effusion lymphoma. Biochem Biophys Res Commun 451, 93-100 crossref(new window)

Zhou H, Li XM, Meinkoth J and Pittman RN (2000) Akt regulates cell survival and apoptosis at a postmitochondrial level. J Cell Biol 151, 483-494 crossref(new window)

Jeong SJ, Dasgupta A, Jung KJ et al (2008) PI3K/AKT inhibition induces caspase-dependent apoptosis in HTLV-1-transformed cells. Virology 370, 264-272 crossref(new window)

Riedl SJ and Shi Y (2004) Molecular mechanisms of caspase regulation during apoptosis. Nat Rev Mol Cell Biol 5, 897-907 crossref(new window)

Wang X, Wang S and Wang J (2010) [Effect of gecko alcohol extract on human esophageal squamous carcinoma cell line EC9706 and anti-tumor activity in vivo]. Zhongguo Zhong Yao Za Zhi 35, 2175-2179

Song JY, Wang XL, Wang JG et al (2011) [Inhibitory effects of Gecko alcohol extract on human esophageal squamous carcinoma cell line EC-109 proliferation and associated mechanism]. Zhong Yao Cai 34, 1020-1023

Ma Y, Zhang B, Qian R, Lu C, Zhao F and Yin L (2006) Tryptase activates PKB in inflammatory reaction in ECV304 cells. Biochim Biophys Acta 1763, 313-321 crossref(new window)

Brown JK, Hollenberg MD and Jones CA (2006) Tryptase activates phosphatidylinositol 3-kinases proteolytically independently from proteinase-activated receptor-2 in cultured dog airway smooth muscle cells. Am J Physiol Lung Cell Mol Physiol 290, L259-269 crossref(new window)

Liao AC, Kuo CC, Huang YC et al (2014) Naringenin inhibits migration of bladder cancer cells through downregulation of AKT and MMP2. Mol Med Rep 10, 1531-1536