Advanced SearchSearch Tips
2,3-Dimethoxy-2′-hydroxychalcone ameliorates TNF-α-induced ICAM-1 expression and subsequent monocyte adhesiveness via NF-kappaB inhibition and HO-1 induction in HaCaT cells
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : BMB Reports
  • Volume 49, Issue 1,  2016, pp.57-62
  • Publisher : Korean Society for Biochemistry and Molecular Biology
  • DOI : 10.5483/BMBRep.2016.49.1.141
 Title & Authors
2,3-Dimethoxy-2′-hydroxychalcone ameliorates TNF-α-induced ICAM-1 expression and subsequent monocyte adhesiveness via NF-kappaB inhibition and HO-1 induction in HaCaT cells
Kim, Hyejin; Youn, Gi Soo; An, Soo Yeon; Kwon, Hyeok Yil; Choi, Soo Young; Park, Jinseu;
  PDF(new window)
Up-regulation of adhesion molecules plays an important role in the infiltration of leukocytes into the skin during the development of various inflammatory skin diseases, such as atopic dermatitis. In this study, we investigated the modulatory effects of 2,3-dimethoxy-2′-hydroxychalcone (DMHC) on tumor necrosis factor (TNF)-α-induced intercellular adhesion molecule-1 (ICAM-1) expression and monocyte adhesiveness, as well as the molecular mechanisms underlying its action in the HaCaT human keratinocyte cell line. Pre-treating HaCaT cells with DMHC significantly suppressed TNF-α-induced ICAM-1 expression and subsequent monocyte adhesiveness. DMHC inhibited TNF-α-induced activation of NF-ᴋB. In addition, DMHC induced HO-1 expression as well as NRF2 activation. Furthermore, HO-1 knockdown using siRNA reversed the inhibitory effect of DMHC on TNF-α-induced ICAM-1 expression and adhesion of monocytes to keratinocytes. These results suggest that DMHC may inhibit TNF-α-induced ICAM-1 expression and adhesion of monocytes to keratinocytes by suppressing the signaling cascades leading to NF-ᴋB activation and inducing HO-1 expression in keratinocytes. [BMB Reports 2016; 49(1): 57-62]
 Cited by
Tat-DJ-1 inhibits oxidative stress-mediated RINm5F cell death through suppression of NF-κB and MAPK activation, Medicinal Chemistry Research, 2016  crossref(new windwow)
Gröne A (2002) Keratinocytes and cytokines. Vet Immunol Immunopathol 88, 1-12 crossref(new window)

Dustin ML, Singer KH, Tuck DT and Springer TA (1988) Adhesion of T lymphoblasts to epidermal keratinocytes is regulated by interferon gamma and is mediated by intercellular adhesion molecule 1 (ICAM-1). J Exp Med 167, 1323-1340 crossref(new window)

Trefzer U, Brockhaus M, Loetscher H et al (1991) 55-kd tumor necrosis factor receptor is expressed by human keratinocytes and plays a pivotal role in regulation of human keratinocyte ICAM-1 expression, J Invest Dermatol 97, 911-916 crossref(new window)

Albanesi C, Scarponi C, Giustizieri ML and Girolomoni G (2005) Keratinocytes in inflammatory skin diseases. Curr Drug Targets Inflamm Allergy 4, 329-334 crossref(new window)

Griffiths CE, Voorhees JJ and Nickoloff BJ (1989) Characterization of intercellular adhesion molecule-1 and HLA-DR expression in normal and inflamed skin: modulation by recombinant gamma interferon and tumor necrosis factor. J Am Acad Dermatol 20, 617-629 crossref(new window)

Matsunaga T, Katayama I, Yokozeki H and Nishioka K (1996) ICAM-1 expression on keratinocytes in mechanically-injured skin of a patient with atopic dermatitis. J Dermatol Sci 12, 219-226 crossref(new window)

Nickoloff BJ, Griffiths CE and Barker JN (1990) The role of adhesion molecules, chemotactic factors, and cytokines in inflammatory and neoplastic skin disease--1990 update. J Invest Dermatol 94, 151S-157S crossref(new window)

Ali S and Mann DA (2004) Signal transduction via the NF-ᴋB pathway: a targeted treatment modality for infection, inflammation and repair. Cell Biochem Funct 22, 67-79 crossref(new window)

Gloire G, Legrand-Poels S and Piette J (2006) NF-ᴋB activation by reactive oxygen species: fifteen years later. Biochem Pharmacol 72, 1493-1505 crossref(new window)

Paine A, Eiz-Vesper B, Blasczyk R and Immenschuh S (2010) Signaling to heme oxygenase-1 and its anti-inflammatory therapeutic potential. Biochem Pharmacol 80, 1895-1903 crossref(new window)

Ryter SW, Alam J and Choi AM (2006) Heme oxygenase-1/carbon monoxide: from basic science to therapeutic applications. Physiol Rev 86, 583-650 crossref(new window)

Listopad J, Asadullah K, Sievers C et al (2007) Heme oxygenase-1 inhibits T cell-dependent skin inflammation and differentiation and function of antigen-presenting cells. Exp Dermatol 16, 661-670 crossref(new window)

Kirino M, Kirino Y, Takeno M et al (2008) Heme oxygenase 1 attenuates the development of atopic dermatitis-like lesions in mice: implications for human disease. J Allergy Clin Immunol 122, 290-297 crossref(new window)

Pae HO, Ha YA, Chai KY and Chung HT (2008) Heme oxygenase-1 attenuates contact hypersensitivity induced by 2,4-dinitrofluorobenzene in mice. Immunopharmacol Immunotoxicol 30, 207-216 crossref(new window)

Bukhari SN, Jantan I and Jasamai M (2013) Anti-inflammatory trends of 1, 3-diphenyl-2-propen-1-one derivatives. Mini Rev Med Chem 13, 87-94 crossref(new window)

Hsieh HK, Lee TH, Wang JP, Wang JJ and Lin CN (1998) Synthesis and anti-inflammatory effect of chalcones and related compounds. Pharm Res 15, 39-46 crossref(new window)

Madan B, Batra S and Ghosh B (2000) 2'-hydroxychalcone inhibits nuclear factor-kappaB and blocks tumor necrosis factor-alpha- and lipopolysaccharide-induced adhesion of neutrophils to human umbilical vein endothelial cells. Mol Pharmacol 58, 526-534

Ban HS, Suzuki K, Lim SS et al (2004) Inhibition of lipopolysaccharide-induced expression of inducible nitric oxide synthase and tumor necrosis factor-alpha by 2'-hydroxychalcone derivatives in RAW 264.7 cells. Biochem Pharmacol 67, 1549-1557 crossref(new window)

Abuarqoub H, Foresti R, Green CJ and Motterlini R (2006) Heme oxygenase-1 mediates the anti-inflammatory actions of 2'-hydroxychalcone in RAW 264.7 murine macrophages. Am J Physiol Cell Physiol 290, C1092-C1099 crossref(new window)

Youn GS, Kwon DJ, Ju SM, Choi SY and Park J (2013) Curcumin ameliorates TNF-α-induced ICAM-1 expression and subsequent THP-1 adhesiveness via the induction of heme oxygenase-1 in the HaCaT cells. BMB Rep 46, 410-415 crossref(new window)

Kwon DJ, Bae YS, Ju SM, Goh AR, Choi SY and Park J (2011) Casuarinin suppresses TNF-α-induced ICAM-1 expression via blockade of NF-ᴋB activation in HaCaT cells. Biochem Biophys Res Commun 409, 780-785 crossref(new window)

Seo WY, Ju SM, Song HY et al (2010) Celastrol suppresses IFN-gamma-induced ICAM-1 expression and subsequent monocyte adhesiveness via the induction of heme oxygenase-1 in the HaCaT cells. Biochem Biophys Res Commun 398, 140-145 crossref(new window)

Shin SY, Kim CG and Lee YH (2013) Egr-1 regulates the transcription of the BRCA1 gene by etoposide. BMB Rep 46, 92-96 crossref(new window)

Kwon DJ, Bae YS, Ju SM, Youn GS, Choi SY and Park J (2014) Salicortin suppresses lipopolysaccharide-stimulated inflammatory responses via blockade of NF-ᴋB and JNK activation in RAW 264.7 macrophages. BMB Rep 47, 318-323 crossref(new window)