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Mitochondrial oxidative phosphorylation complexes exist in the sarcolemma of skeletal muscle
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  • Journal title : BMB Reports
  • Volume 49, Issue 2,  2016, pp.116-121
  • Publisher : Korean Society for Biochemistry and Molecular Biology
  • DOI : 10.5483/BMBRep.2016.49.2.232
 Title & Authors
Mitochondrial oxidative phosphorylation complexes exist in the sarcolemma of skeletal muscle
Lee, Hyun; Kim, Seung-Hyeob; Lee, Jae-Seon; Yang, Yun-Hee; Nam, Jwa-Min; Kim, Bong-Woo; Ko, Young-Gyu;
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Although proteomic analyses have revealed the presence of mitochondrial oxidative phosphorylation (OXPHOS) proteins in the plasma membrane, there have been no in-depth evaluations of the presence or function of OXPHOS I-V in the plasma membrane. Here, we demonstrate the in situ localization of OXPHOS I-V complexes to the sarcolemma of skeletal muscle by immunofluorescence and immunohistochemistry. A portion of the OXPHOS I-V complex proteins was not co-stained with MitoTracker but co-localized with caveolin-3 in the sarcolemma of mouse gastrocnemius. Mitochondrial matrix-facing OXPHOS complex subunits were ectopically expressed in the sarcolemma of the non-permeabilized muscle fibers and C2C12 myotubes. The sarcolemmal localization of cytochrome c was also observed from mouse gastrocnemius muscles and C2C12 myotubes, as determined by confocal and total internal resonance fluorescence (TIRF) microscopy. Based on these data, we conclude that a portion of OXPHOS complexes is localized in the sarcolemma of skeletal muscle and may have non-canonical functions.
Cytochrome c;Extracellular NADH-dependent respiration;Oxidative phosphorylation complexes;Sarcolemma;Skeletal muscle;
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