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The Effect of Rotating Magnetic Field on Enterotoxin Genes Expression in Staphylococcus Aureus Strains
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  • Journal title : Journal of Magnetics
  • Volume 21, Issue 1,  2016, pp.141-147
  • Publisher : The Korean Magnetics Society
  • DOI : 10.4283/JMAG.2016.21.1.141
 Title & Authors
The Effect of Rotating Magnetic Field on Enterotoxin Genes Expression in Staphylococcus Aureus Strains
Fijalkowski, Karol; Peitler, Dorota; Zywicka, Anna; Rakoczy, Rafal;
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 Abstract
Staphylococcus aureus cultures exposed to rotating magnetic field (RMF) were studied in order to analyse the possible induced changes in staphylococcal enterotoxin genes (se) expression. Liquid cultures of S. aureus strains carrying different se were exposed to the RMF of magnetic frequency 50 Hz and magnetic induction 34 mT for 10 h at . Three time points of bacterial growth cycle were considered for RNA extractions. Gene expression analyses were evaluated using real-time quantitative PCR method. The present study confirmed, that the RMF can stimulate the growth rate of S. aureus cultures in comparison to the unexposed controls, while the stimulation is not strain dependent. The studies have also shown, that the RMF, depending on the exposure time but regardless the bacterial strain, can influence on the expression of various se. In general, except for sea, as a result of bacterial exposure to the RMF through subsequent growth phases, the expression of se decreased, reaching the values below results recorded for unexposed controls. In the case of sea expression remained at a lower level as compared to the control, regardless the time of exposition.
 Keywords
rotating magnetic field;Staphylococcus aureus;gene expression;enterotoxin;real-time PCR;
 Language
English
 Cited by
 References
1.
H. Lin, M. Opler, M. Head, M. Blank, and R. Goodman, J. Cell. Biochem. 66, 482 (1997). crossref(new window)

2.
J. L. Phillips, W. Haggren, W. J. Thomas, T. Ishida-Jones, and W. R. Adey, Biochem. Biophys. Acta. 1132, 140 (1992).

3.
H. Lin, M. Blank, M. Jin, and R. Goodman, Bioelectrochem. Bioenerg. 39, 215 (1996). crossref(new window)

4.
R. Goodman and M. Blank, J. Cell. Physiol. 192, 16 (2002). crossref(new window)

5.
L. Fojt, P. Klapetek, L. Strasak, and V. Vetterl, Micron. 40, 18 (2009).

6.
F. D. Matl, A. Obermeier, J. Zlotnyk, W. Friess, A. Stemberger, and R. Burgkart, Bioelectromagnetics 32, 367 (2011). crossref(new window)

7.
L. Potenza, L. Ubaldi, R. De Sanctis, R. De Bellis, L. Cucchiarini, and M. Dacha, Mutat. Res. 561, 53 (2004). crossref(new window)

8.
Y. Le Loir, F. Baron, and M. Gautier, Gent. Mol. Res. 2, 63 (2003).

9.
M. A. Argudin, M. C. Mendoza, and M. Rosario-Rodicio, Toxins (Basel) 2, 1751 (2010). crossref(new window)

10.
M. E. Marin, M. C. de la Rosa, and I. Cornejo, Appl. Environ. Microbiol. 58, 1067 (1992).

11.
J. Schelin, N. Wallin-Carlquist, M. Thorup Cohn, R. Lindqvist, G. C. Barker, and P. Radstrom, Virulence 2, 580 (2011). crossref(new window)

12.
R. Rakoczy, Chem. Eng. Process. 66, 1 (2013). crossref(new window)

13.
D. R. Patrick and S. W. Fardo, Rotating Electrical Machines and Power Systems (2nd ed.) Fairmont Press USA (1997) pp. 279-324.

14.
J. F. Jr Karkos, and R. Flanary, CA 2505561 A1 (2003).

15.
J. F. Jr Karkos, and R. Flanary, US 20100214867 A1 (2009).

16.
J. F. Jr Karkos, and R. Flanary, DE 60317091 D1 (2003).

17.
D. Wu, X. Li, Y. Yang, Y. Zheng, C. Wang, L. Deng, L. Liu, C. Li, Y. Shang, C. Zhao, S. Yu, and X. Shen, J. Med. Microbiol. 60, 35 (2011). crossref(new window)

18.
M. Kuroda, T. Ohta, I. Uchiyama, T. Baba, H. Yuzawa, I. Kobayashi, L. Cui, A. Oguchi, K. Aoki, Y. Nagai, J. Lian, T. Ito, M. Kanamori, H. Matsumaru, A. Maruyama, H. Murakami, A. Hosoyama, Y. Mizutani-Ui, N.K. Takahashi, T. Sawano, R. Inoue, C. Kaito, K. Sekimizu, H. Hirakawa, S. Kuhara, S. Goto, J. Yabuzaki, M. Kanehisa, A. Yamashita, K. Oshima, K. Furuya, C. Yoshino, T. Shiba, M. Hattori, N. Ogasawara, H. Hayashi, and K. Hiramatsu, Lancet 21, 1225 (2001).

19.
S. Holtfreter, D. Grumann, M. Schmudde, N. T. Nguyen, P. Eichler, B. Strommenger, K. Kopron, J. Kolata, S. Giedrys-Kalemba, I. Steinmetz, W. Witte, and B. M. Broker, J. Clin. Microbiol. 45, 2669 (2007). crossref(new window)

20.
K. Fijalkowski, P. Nawrotek, M. Struk, M. Kordas, and R. Rakoczy, J. Magn. 18, 289 (2013). crossref(new window)

21.
M. Duquenne, I. Fleurot, M. Aigle, C. Darrigo, E. Borezee-Durant, S. Derzelle, M. Bouix V. Deperrois-Lafarge, and A. Delacroix-Buchet, Appl. Environ. Microbiol. 76, 1367 (2010). crossref(new window)

22.
S. Derzelle, F. Dilasser, M. Duquenne, and V. Deperrois, Food Microbiol. 26, 896 (2009). crossref(new window)

23.
M. W. Pfaffl, A. Tichopad, C. Prgomet, and T. P. Neuvians, Biotechnol. Lett. 26, 509 (2004). crossref(new window)

24.
J. Vandesompele, K. De Preter, F. Pattyn, B. Poppe, N. Van Roy, A. De Paepe, and F. Speleman, Genome Biol. 3, 34 (2002).

25.
J. Filipic, B. Kraigher, B. Tepus, V. Kokol, and I. Mandic-Mulec, Bioresour. Technol. 120, 225 (2012). crossref(new window)

26.
G. Giorgi, P. Marcantonio, F. Bersani, E. Gavoci, and B. Del Re, Int. J. Radiat. Biol. 87, 601 (2011). crossref(new window)

27.
L. Potenza, L. Cucchiarini, E. Piatti, U. Angelini, and M. Dacha, Bioelectromagnetics 25, 352 (2004). crossref(new window)

28.
S. H. Li and K. C. Chow, Biochem. Biophys. Res. Commun. 280, 1385 (2001). crossref(new window)

29.
W. R. Schwan, L. Lehmann, and J. McCormick, Infect. Immun. 74, 399 (2006). crossref(new window)

30.
S. F. Pereira, A. O. Henriques, M. G. Pinho, H. De Lencastre, and A. Tomasz, J. Bacteriol. 189, 3525 (2007). crossref(new window)

31.
Y. Chien, A. C. Manna, S. J. Projan, and A. L. Cheung, J. Biol. Chem. 274, 37169 (1999). crossref(new window)

32.
C. Goerke, M. G. Bayer, and C. Wolz, Clin. Diagn. Lab. Immunol. 8, 279 (2001).

33.
O. Thellin, W. Zorzi, B. Lakaye, B. De Borman, B. Coumans, G. Hennen, T. Grisar, A. Igout, and E. Heinen, J. Biotechnol. 75, 291 (1999). crossref(new window)

34.
K. Dheda, J. F. Huggett, S. A. Bustin, M. A. Johnson, G. Rook, and A. Zumla, Biotechniques 37, 118 (2004).

35.
S. J. Vandecasteele, W. E. Peetermans, R. Merckx, and J. Van Eldere, J. Bacteriol. 183, 7094 (2001). crossref(new window)

36.
M. T. Tremaine, D. K. Brockman, and M. J. Betley, Infect. Immun. 61, 356 (1993).

37.
L. B. Regassa, J. L. Couch, and M. J. Betley, Infect. Immun. 59, 955 (1991).

38.
S. Even, C. Charlier, S. Nouaille, N. L. Ben Zakour, M. Cretenet, F. J. Cousin, M. Gautier, M. Cocaign-Bousquet, P. Loubiere, and Y. Le Loir, Appl. Environ. Microbiol. 75, 4459 (2009). crossref(new window)