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Characterizations of Two-step Matrix Application Procedures for Imaging Mass Spectrometry
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  • Journal title : Mass Spectrometry Letters
  • Volume 6, Issue 1,  2015, pp.21-25
  • Publisher : Korean Society Mass Spectrometry
  • DOI : 10.5478/MSL.2015.6.1.21
 Title & Authors
Characterizations of Two-step Matrix Application Procedures for Imaging Mass Spectrometry
Shimma, Shuichi;
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In this paper, I describe the importance of matrix spraying conditions in imaging mass spectrometry (IMS) to obtain successful imaging results. My developed matrix application methodology, which is a "two-step matrix application" sequentially combined with matrix sublimation and spraying matrix solution can provide high reproducibility and high ion yield compared with a conventional direct spraying method. However, insufficient IMS results were obtained occasionally despite the two-step method. Therefore, I wanted to characterize the methodology to continuously provide high quality data. According to my results, the sublimation time was not a strict parameter, and the most important step was the first spraying condition. This means that the extraction conditions from the tissue section and co-crystallization of the matrix were the most important factors.
imaging mass spectrometry;mass microscope;matrix application;tumor;anti-cancer agents;sample preparations;
 Cited by
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