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Mass Spectrometric Determination of Zn2+ Binding/Dissociation Constant for Zinc Finger Peptides
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  • Journal title : Mass Spectrometry Letters
  • Volume 6, Issue 1,  2015, pp.7-12
  • Publisher : Korean Society Mass Spectrometry
  • DOI : 10.5478/MSL.2015.6.1.7
 Title & Authors
Mass Spectrometric Determination of Zn2+ Binding/Dissociation Constant for Zinc Finger Peptides
Lee, Choong Sik; Park, Soo Jin; Lee, Jae Young; Park, Sungsu; Jo, Kyubong; Oh, Han Bin;
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 Abstract
In the present study, we proposed a simple ESI-MS model for determining binding (or dissociation) constants for zinc finger peptides (ZFPs) with a unique fold consensus. The ionization efficiency (response) factors for this model, i.e., and , could be determined for ZiCo ZFP with a known binding constant. We could determine the binding constants for other ZFPs assuming those with a consensus conformation have the same response ratio. In general, the ZPF dissociation constants exhibited values of , while values for a negative control non-specific peptides were high, e.g., and for BBA1 and melittin, respectively.
 Keywords
zinc finger;binding constant;electrospray-mass spectrometry;zinc ion;
 Language
English
 Cited by
1.
Soft Ionization of Metallo-Mefenamic Using Electrospray Ionization Mass Spectrometry,;;

Mass Spectrometry Letters, 2015. vol.6. 2, pp.43-47 crossref(new window)
1.
Soft Ionization of Metallo-Mefenamic Using Electrospray Ionization Mass Spectrometry, Mass Spectrometry Letters, 2015, 6, 2, 43  crossref(new windwow)
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