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Mass Spectrometric Determination of Zn2+ Binding/Dissociation Constant for Zinc Finger Peptides
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  • Journal title : Mass Spectrometry Letters
  • Volume 6, Issue 1,  2015, pp.7-12
  • Publisher : Korean Society Mass Spectrometry
  • DOI : 10.5478/MSL.2015.6.1.7
 Title & Authors
Mass Spectrometric Determination of Zn2+ Binding/Dissociation Constant for Zinc Finger Peptides
Lee, Choong Sik; Park, Soo Jin; Lee, Jae Young; Park, Sungsu; Jo, Kyubong; Oh, Han Bin;
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In the present study, we proposed a simple ESI-MS model for determining binding (or dissociation) constants for zinc finger peptides (ZFPs) with a unique fold consensus. The ionization efficiency (response) factors for this model, i.e., and , could be determined for ZiCo ZFP with a known binding constant. We could determine the binding constants for other ZFPs assuming those with a consensus conformation have the same response ratio. In general, the ZPF dissociation constants exhibited values of , while values for a negative control non-specific peptides were high, e.g., and for BBA1 and melittin, respectively.
zinc finger;binding constant;electrospray-mass spectrometry;zinc ion;
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