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MS Platform for Erythropoietin Glycome Characterization
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  • Journal title : Mass Spectrometry Letters
  • Volume 6, Issue 3,  2015, pp.53-58
  • Publisher : Korean Society Mass Spectrometry
  • DOI : 10.5478/MSL.2015.6.3.53
 Title & Authors
MS Platform for Erythropoietin Glycome Characterization
Seo, Youngsuk; Kim, Unyong; Oh, Myung Jin; Yun, Na Young; An, Hyun Joo;
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Recombinant erythropoietins (EPOs) are an important class of biotherapeutics that stimulate red blood cell production. The quality, safety, and potency of EPO variants are determined largely by their glycosylation, which makes up nearly half their mass. Thus, detailed glycomic analyses are important to assess biotherapeutic quality and establish the equivalency of biosimilar EPOs now coming to market. High-resolution mass spectrometry (MS) has recently emerged as the premier tool for glycan analysis in EPOs. Using the accurate mass measurements provided by high-resolution MS, the compositions of even large, complex glycans can easily be determined. When combined with a nano-LC separation, differentiation of structural isomers also becomes a possibility. These components, together, provide a comprehensive picture of biotherapeutic glycosylation. In this review, we provide an overview of MS-based analytical platform for glycomic characterization of EPO biotherapeutics and biosimilars.
Mass spectrometry;Erythropoietin;Glycosylation;Glycomics;
 Cited by
Sensitive and comprehensive analysis of O-glycosylation in biotherapeutics: a case study of novel erythropoiesis stimulating protein, Bioanalysis, 2017, 9, 18, 1373  crossref(new windwow)
Jelkmann, W. Transfus. Med. Hemother. 2013, 40, 302. crossref(new window)

Egrie, J.; Grant, J.; Gillies, D.; Aoki, K.; Strickland, T. Glycoconjugate J. 1993, 10, 263. crossref(new window)

Corfield, A. P.; Sander-Wewer, M.; Veh, R. W.; Wember, M.; Schauer, R. Biol. Chem. 1986, 367, 433.

Oh, M. J.; Hua, S.; Kim, B. J.; Jeong, H. N.; Jeong, S. H.; Grimm, R.; Yoo, J. S.; An, H. J. Bioanalysis 2013, 5, 545. crossref(new window)

Hua, S.; Oh, M. J.; Ozcan, S.; Seo, Y. S.; Grimm, R.; An, H. J. TrAC Trends in Analytical Chemistry 2015, 68, 18. crossref(new window)

Domann, P. J.; Pardos-Pardos, A. C.; Fernandes, D. L.; Spencer, D. I. R.; Radcliffe, C. M.; Royle, L.; Dwek, R. A.; Rudd, P. M. Proteomics 2007, 7, 70. crossref(new window)

Harvey, D. J. Expert Rev. Proteomics 2005, 2, 87. crossref(new window)

Hua, S.; Oh, M. J.; An, H. J. Mass Spectrometry Letters 2013, 4, 10. crossref(new window)

An, H. J.; Lebrilla, C. B. Mass Spectrom. Rev. 2011, 30, 560. crossref(new window)

Beck, A.; Debaene, F.; Diemer, H.; Wagner-Rousset, E.; Colas, O.; Dorsselaer, A. V.; Cianférani, S. J. Mass Spectrom. 2015, 50, 285. crossref(new window)

Jensen, P. F.; Larraillet, V.; Schlothauer, T.; Kettenberger, H.; Hilger, M.; Rand, K. D. Mol. Cell. Proteomics 2015, 14, 148. crossref(new window)

Song, T.; Ozcan, S.; Becker, A.; Lebrilla, C. B. Anal. Chem. 2014, 86, 5661. crossref(new window)

Jiang, J.; Tian, F.; Cai, Y.; Qian, X.; Costello, C. E.; Ying, W. Anal. Bioanal. Chem. 2014, 406, 6265. crossref(new window)

Hashii, N.; Harazono, A.; Kuribayashi, R.; Takakura, D.; Kawasaki, N. Rapid Commun. Mass Spectrom. 2014, 28, 921. crossref(new window)

Schröder, S.; Matthes, F.; Hyden, P.; Andersson, C.; Fogh, J.; Müller-Loennies, S.; Braulke, T.; Gieselmann, V.; Matzner, U. Glycobiology 2010, 20, 248. crossref(new window)

Lee, J.-E.; Kim, C.; Yang, H.; Park, I.; Oh, N.; Hua, S.; Jeong, H.; An, H. J.; Kim, S. C.; Lee, G. M. Mol. Cancer Ther. 2015, 14, 470. crossref(new window)

Balaguer, E.; Demelbauer, U.; Pelzing, M.; Sanz-Nebot, V.; Barbosa, J.; Neusüß, C. Electrophoresis 2006, 27, 2638. crossref(new window)

Melmer, M.; Stangler, T.; Schiefermeier, M.; Brunner, W.; Toll, H.; Rupprechter, A.; Lindner, W.; Premstaller, A. Anal. Bioanal. Chem. 2010, 398, 905. crossref(new window)

Guile, G. R.; Rudd, P. M.; Wing, D. R.; Prime, S. B.; Dwek, R. A. Anal. Biochem. 1996, 240, 210. crossref(new window)

Llop, E.; Gallego, R. G.; Belalcazar, V.; Gerwig, G. J.; Kamerling, J. P.; Segura, J.; Pascual, J. A. Proteomics 2007, 7, 4278. crossref(new window)

Hua, S.; Lebrilla, C.; An, H. J. Bioanalysis 2011, 3, 2573. crossref(new window)

Hua, S.; Williams, C. C.; Dimapasoc, L. M.; Ro, G. S.; Ozcan, S.; Miyamoto, S.; Lebrilla, C. B.; An, H. J.; Leiserowitz, G. S. J. Chromatogr. A 2013, 1279, 58. crossref(new window)

Hua, S.; Jeong, H. N.; Dimapasoc, L. M.; Kang, I.; Han, C.; Choi, J.-S.; Lebrilla, C. B.; An, H. J. Anal. Chem. 2013, 85, 4636. crossref(new window)

Huang, Y.; Mechref, Y.; Novotny, M. V. Anal. Chem. 2001, 73, 6063. crossref(new window)

Robbe, C.; Capon, C.; Flahaut, C.; Michalski, J. C. Electrophoresis 2003, 24, 611. crossref(new window)

Maniatis, S.; Zhou, H.; Reinhold, V. Anal. Chem. 2010, 82, 2421. crossref(new window)

Harvey, D. J. Mass Spectrom. Rev. 2015, 34, 268. crossref(new window)

An, H. J.; Kronewitter, S. R.; de Leoz, M. L. A.; Lebrilla, C. B. Curr. Opin. Chem. Biol. 2009, 13, 601. crossref(new window)

Medzihradszky, K. F.; Kaasik, K.; Chalkley, R. J. Anal. Chem. 2015, 3064. crossref(new window)

An, H. J.; Froehlich, J. W.; Lebrilla, C. B. Curr. Opin. Chem. Biol. 2009, 13, 421. crossref(new window)

Gawlitzek, M.; Estacio, M.; Furch, T.; Kiss, R. Biotechnol. Bioeng. 2009, 103, 1164. crossref(new window)

Ohta, M.; Kawasaki, N.; Itoh, S.; Hayakawa, T. Biologicals 2002, 30, 235. crossref(new window)

Rush, R. S.; Derby, P. L.; Smith, D. M.; Merry, C.; Rogers, G.; Rohde, M. F.; Katta, V. Anal. Chem. 1995, 67, 1442. crossref(new window)

Cointe, D.; Beliard, R.; Jorieux, S.; Leroy, Y.; Glacet, A.; Verbert, A.; Bourel, D.; Chirat, F. Glycobiology 2000, 10, 511. crossref(new window)

Bones, J.; McLoughlin, N.; Hilliard, M.; Wynne, K.; Karger, B. L.; Rudd, P. M. Anal. Chem. 2011, 83, 4154. crossref(new window)

Takegawa, Y.; Ito, H.; Keira, T.; Deguchi, K.; Nakagawa, H.; Nishimura, S. I. J. Sep. Sci. 2008, 31, 1585. crossref(new window)

Dezutter-Dambuyant, C.; Schmitt, D.; Dusserre, N.; Hanau, D.; Kolbe, H.; Kieny, M.-P.; Gazzolo, L.; Mace, K.; Pasquali, J.-L.; Olivier, R. Res. Virol. 1991, 142, 129. crossref(new window)

Bezouška, K.; Sklenáø, J.; Novák, P.; Halada, P.; Havlíèek, V.; Kraus, M.; Tichá, M.; Jonáková, V. Protein Sci. 1999, 8, 1551. crossref(new window)

Froehlich, J. W.; Barboza, M.; Chu, C.; Lerno, L. A.; Clowers, B. H.; Zivkovic, A. M.; German, J. B.; Lebrilla, C. B. Anal. Chem. 2011, 83, 5541. crossref(new window)

Nwosu, C. C.; Seipert, R. R.; Strum, J. S.; Hua, S. S.; An, H. J.; Zivkovic, A. M.; German, B. J.; Lebrilla, C. B. J. Proteome Res. 2011, 10, 2612. crossref(new window)

Yu, Y. Q.; Fournier, J.; Gilar, M.; Gebler, J. C. Anal. Chem. 2007, 79, 1731. crossref(new window)

An, H. J.; Peavy, T. R.; Hedrick, J. L.; Lebrilla, C. B. Anal. Chem. 2003, 75, 5628. crossref(new window)

Clowers, B. H.; Dodds, E. D.; Seipert, R. R.; Lebrilla, C. B. J. Proteome Res. 2007, 6, 4032. crossref(new window)

Hua, S.; Nwosu, C.; Strum, J.; Seipert, R.; An, H.; Zivkovic, A.; German, J.; Lebrilla, C. Anal. Bioanal. Chem. 2012, 403, 1291. crossref(new window)

Hua, S.; Hu, C. Y.; Kim, B. J.; Totten, S. M.; Oh, M. J.; Yun, N.; Nwosu, C. C.; Yoo, J. S.; Lebrilla, C. B.; An, H. J. J. Proteome Res. 2013, 12, 4414. crossref(new window)