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MS Platform for Erythropoietin Glycome Characterization
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  • Journal title : Mass Spectrometry Letters
  • Volume 6, Issue 3,  2015, pp.53-58
  • Publisher : Korean Society Mass Spectrometry
  • DOI : 10.5478/MSL.2015.6.3.53
 Title & Authors
MS Platform for Erythropoietin Glycome Characterization
Seo, Youngsuk; Kim, Unyong; Oh, Myung Jin; Yun, Na Young; An, Hyun Joo;
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 Abstract
Recombinant erythropoietins (EPOs) are an important class of biotherapeutics that stimulate red blood cell production. The quality, safety, and potency of EPO variants are determined largely by their glycosylation, which makes up nearly half their mass. Thus, detailed glycomic analyses are important to assess biotherapeutic quality and establish the equivalency of biosimilar EPOs now coming to market. High-resolution mass spectrometry (MS) has recently emerged as the premier tool for glycan analysis in EPOs. Using the accurate mass measurements provided by high-resolution MS, the compositions of even large, complex glycans can easily be determined. When combined with a nano-LC separation, differentiation of structural isomers also becomes a possibility. These components, together, provide a comprehensive picture of biotherapeutic glycosylation. In this review, we provide an overview of MS-based analytical platform for glycomic characterization of EPO biotherapeutics and biosimilars.
 Keywords
Mass spectrometry;Erythropoietin;Glycosylation;Glycomics;
 Language
English
 Cited by
1.
Sensitive and comprehensive analysis of O-glycosylation in biotherapeutics: a case study of novel erythropoiesis stimulating protein, Bioanalysis, 2017  crossref(new windwow)
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