JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Evaluation and Assessment of Biosafety for Bacillus thuringiensis (Bt)-transgenic Rice: Responses of Daphnia magna Fed on Bt-transgenic Rice Variety
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Evaluation and Assessment of Biosafety for Bacillus thuringiensis (Bt)-transgenic Rice: Responses of Daphnia magna Fed on Bt-transgenic Rice Variety
Oh, Sung-Dug; Shin, Hye-Chul; Sohn, Soo-In; Lee, Ki-Jong; Kim, Hyo-Jin; Ryu, Tae-Hun; Lee, Jang-Yong; Park, Beom-Seok; Kweon, Soon-Jong; Suh, Seok-Cheol; Park, Jong-Sug;
  PDF(new window)
 Abstract
Insect-resistant transgenic rice was developed by inserting the mCry1Ac1 a modified gene from the soil bacterium Bacillus thuringiensis (Bt). For biosafety assessment, we studied the effects on survival of cantor Daphnia magna, a commonly used as a model organism in ecotoxicological studies. D. magna fed on Bt rice and its near non-genetically modified (GM) counterparts (Nakdong) grown in the same environment (100% ground rice suspension). The Bt rice was comfirmed to have the insertion of T-DNA and protein expression by the polymerase chain reaction and ELISA analysis. Feeding study showed similar cumulative immobility and abnormal response of D. magna between Bt rice and non-GM counterparts. 48 h- values of Bt rice and non-GM rice showed 4,429 and 2,889 mg/L respectively. The rice no observed effect concentration (NOEC) values for D. magna was suggested 1,000 mg/L. We conclude that the tested Bt-rice and Nakdong similar cumulative immobility for D. magna the widely used model organism. We found out that there is strong possibility that the growth of Bt rice didn`t affect to non-target insects.
 Keywords
Bacillus thuringiensis rice;Daphnia magna;Environment risk;
 Language
Korean
 Cited by
1.
비타민 A 강화벼 급이가 벼물바구미(Lissorhoptrus oryzophilus)의 살충제 감수성에 미치는 영향,오성덕;이기종;박수윤;류태훈;김재광;손수인;김진서;하선화;박종석;안병옥;조현석;서상재;

한국환경농학회지, 2012. vol.31. 3, pp.286-292 crossref(new window)
2.
비타민A 강화 벼 분자생물학적 분석 및 물벼룩 급이 효과,오성덕;이기종;박수윤;손수인;류태훈;김재광;김진서;안홍일;하선화;박종석;안병옥;조현석;서상재;

한국국제농업개발학회지, 2012. vol.24. 4, pp.477-484
3.
해충저항성 Bacillus thuringiensis (Bt) 벼 급이가 벼물바구미(Lissorhoptrus oryzophilus)의 살충제 감수성에 미치는 영향,오성덕;이기종;손수인;권용정;김진서;이장용;박범석;권순종;서석철;류태훈;박종석;안병옥;조현석;서상재;

한국국제농업개발학회지, 2012. vol.24. 2, pp.247-253
4.
비타민A 강화벼의 환경위해성 평가 : 4세대의 후대안정성 연구,오성덕;박수윤;손수인;김재광;박종석;박순기;조현석;안병옥;이기종;

한국육종학회지, 2012. vol.44. 4, pp.579-587 crossref(new window)
5.
병저항성 GM(OsCK1)벼가 미꾸리(Misgurnus anguillicaudatus)및 잉어(Cyprinus carpio)에 미치는 영향,오성덕;이기종;박수윤;이대용;손수인;김민영;류태훈;

한국환경농학회지, 2013. vol.32. 3, pp.231-239 crossref(new window)
6.
병저항성 GM(OsCK1)벼의 물벼룩(Daphnia magna)에 대한 급성독성 평가,오성덕;이기종;박수윤;류태훈;서상재;

한국환경농학회지, 2014. vol.33. 2, pp.121-128 crossref(new window)
7.
Bt 양배추가 복숭아혹진딧물의 발육과 기주선택에 미치는 영향,남기정;김영중;문두범;남경희;백인순;박정호;정순천;한지학;김창기;

한국응용곤충학회지, 2014. vol.53. 2, pp.193-197 crossref(new window)
8.
Acute toxicity evaluation of drought-tolerant transgenic rice Agb0103 to Daphnia magna,;;;;;;;

농업과학연구, 2016. vol.43. 2, pp.205-214 crossref(new window)
9.
유전자 변형 비타민E 강화콩의 환경 영향 평가 : 비타민E 강화콩의 급이가 물벼룩(Daphnia magna)에 미치는 영향,오성덕;윤도원;손수인;이범규;이기종;장안철;

한국국제농업개발학회지, 2016. vol.28. 2, pp.197-204 crossref(new window)
1.
Responses of Misgurnus anguillicaudatus and Cyprinus carpio Fed on Disease Resistant(OsCK1) Rice Variety, Korean Journal of Environmental Agriculture, 2013, 32, 3, 231  crossref(new windwow)
2.
Acute Toxicity Evaluation to Daphnia magna of Disease Resistant(OsCK1) Rice, Korean Journal of Environmental Agriculture, 2014, 33, 2, 121  crossref(new windwow)
3.
Effects of Bt Cabbage (Brassica oleracea) on the Host Preference and Performance of the Green Peach Aphid, Myzus persicae Sulzer (Hemiptera: Aphididae), Korean journal of applied entomology, 2014, 53, 2, 193  crossref(new windwow)
 References
1.
Aronson AI and Shai Y (2001) Why Bacillus thuringiensis insecticidal toxins are so effective: Unique features of their mode of action. FEMS Microbiol Lett 195, 1-8. crossref(new window)

2.
Betz FS, Hammond BG, and Fuchs RL (2000) Safety and advantages of Bacillus thuringiensis-protected plants to control insect pests. Regul Toxicol Pharmacol 32, 156-173. crossref(new window)

3.
Bohn T, Primicerio R, Hessen DO, and Traavik T (2008) Reduced fitness of Daphnia magna fed a Bt-transgenic maize variety. Arch Environ Contam Toxicol 55, 584-592. crossref(new window)

4.
Bravo A, Gill SS, and Soberon M (2007) Modeof action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon 49, 423-435. crossref(new window)

5.
Crickmore N (2005) Using worms to better understand how Bacillus thuringiensis kills insects. Trends Microbiol 13, 347-350. crossref(new window)

6.
de Vries J and Wackernagel W (2004) Microbial horizontal gene transfer and the DNA release from transgenic crop plants. Plant and Soil 266, 91-104.

7.
Jensen PD, Dively GP, Swan CM, and Lamp WO (2010) Exposure and nontarget Effects of Transgenic Bt Corn Debris in Streams. Environmental Entomology 39, 707-714. crossref(new window)

8.
Kim KY, Kim KR, and Lee SI (2010a) Acute toxicity test for heavy metals using water fleas. J Korean Wood Sci & Tech 18, 37-47.

9.
Kim HJ, Lee SM, Kim JK, Ryu TH, Suh SC, and Cho HS (2010b) Expression of PAT and NPT II proteins during the developmental stages of a genetically modified pepper developed in Korea. J Agric Food Chem 58, 10906-10910. crossref(new window)

10.
Kramer KJM, Jak RG, van Hattum B, Hooftman RN, and Zwolsman JJG (2004) Copper toxicity in relation to surface water-dissolved organic matter: biological effects to Daphnia magna. Environ Toxicol Chem 23, 2971-2980. crossref(new window)

11.
Lee CW, Ryu JY, and Lim KW (2007) Acute Toxicity Test of Agricultural Chemicals to Water Fleas. J Environ Sci 16, 55-63. crossref(new window)

12.
Mendelsohn M, Kough J, Vaituzis Z, and Matthews K (2003) Are Bt safe? Nature Biotechnology 21, 1003-1009. crossref(new window)

13.
Nap JP, Bijvoet J, and Stiekema WJ (1992) Biosafety of kanamycin-resistant transgenic plants. Transgenic Res 1, 239-249. crossref(new window)

14.
Nguyen HT and Jehle JA (2009) Stability of Cry1Ab protein during long-term storage for standardization of insect bioassays. Environ Biosafety Res 8, 113-119. crossref(new window)

15.
Schnepf HE and Whiteley HR (1981) Cloning and expression of the Bacillus thuringiensis crystal protein gene in Escherichia coli. PNAS 78, 2893-2897 crossref(new window)

16.
Shin KS, Lee SM, Lim SH, Woo HJ, Cho HS, Lee KR, Lee MC, Kweon SJ, and Suh SC (2009) Research articles: Molecular biological characteristics and analysis using the specific markers of leaf folder-resistant GM rice. Plant Biotechnol 36, 115-123. crossref(new window)

17.
Sohn SI, Oh YJ, Oh SD, Kim MK, Ryu TH, Lee KJ, Suh SC, Baek HJ, and Park JS (2010) Molecular analysis of microbial community in soils cultivating Bt chinese cabbage. Korean Environ Agri 29, 293-299. crossref(new window)

18.
Thomas Bøhn, Terje Traavik, and Raul Primicerio. (2010) Demographic responses of Daphnia magna fed transgenic Bt-maize. Ecotoxicology 19, 419-430. crossref(new window)

19.
Versteeg DJ, Stalmans MSD, and Janssen C (1997) Ceriodaphnia and Daphnia: A comparison of their sensitivity to xenobiotics and utility as a test species. Chemosphere 34, 869-892. crossref(new window)