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Overexpression of Ice Recrystallization Inhibition Protein (HvIRIP) from Barley Enhances Cold Tolerance in Transgenic rapeseed plants
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 Title & Authors
Overexpression of Ice Recrystallization Inhibition Protein (HvIRIP) from Barley Enhances Cold Tolerance in Transgenic rapeseed plants
Roh, Kyung Hee; Park, Jong-Sug; Kang, Han-Chul; Kim, Jong-Bum; Jang, Young-Suk; Kim, Kwang-Soo; Yi, Hankuil;
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 Abstract
Rapeseed (Brassica napus) is now the second largest oilseed crop after soybean. Cold temperature tolerance is an important agronomic trait in winter rapeseed that determines the plant's ability to control below freezing temperatures. To improve cold tolerance of rapeseed plants, an expression vector containing an Barley Ice recrystallization inhibition protein (HvIRIP) cDNA driven by a cauliflower mosaic virus 35S promoter was transferred into rapeseed plants. Transgenic expression of HvIRIP was proved by southern- and northern-blot analyses. The level of freezing tolerance of transgenic plants was found to be significantly greater than that of wild-type rapeseed plants by freezing assay. Proline accumulation during cold stress was also highly induced in the transgenic rapeseed plants. The transgenic plants exhibited considerable tolerance against oxidative damage induced by cold stress. Our results indicated that heterologous HvIRIP expression in transgenic rapeseed plants may induce several oxidative-stress responsive genes to protect from cold stress.
 Keywords
antioxidant enzyme activity;Brassica napus;cold tolerance;HvIRIP;ice recrystallization inhibition protein;rapeseed;
 Language
Korean
 Cited by
 References
1.
Atici O and Nalbantoglu B (2003) Antifreeze proteins in higher plants. Phytochemistry 64, 1187-96. crossref(new window)

2.
Bates LS (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39, 205-7. crossref(new window)

3.
Booth EJ and Gunstone FD (2004) Rapeseeds and rapeseed oil; agronomy, production, and trade. In Rapeseed and Canola oil, Gunstone FD, pp. 1- 16. Blackwell publishing Ltd., UK.

4.
Brandts JF, Brennan M, and Lin LN (1977) Unfolding and refolding occur much faster for a proline-free protein than for most proline-containing proteins. Proc Natl Acad Sci USA 74, 4178-81. crossref(new window)

5.
Chew O, Lelean S, John UP, and Spangenberg GC (2012) Cold acclimation induces rapid and dynamic changes in freeze tolerance mechanisms in the cryophile Deschampsia antarctica E. Desv. Plant Cell Environ 35, 829-37. crossref(new window)

6.
Chow PS and Landhausser SM (2004) A method for routine measurements of total sugar and starch content in woody plant tissues. Tree Physiol 24, 1129-36. crossref(new window)

7.
Christie P, Hahn M, and Walbot V (1991) Low-temperature accumulation of alcohol dehydrogenase-1 mRNA and protein activity and Maize and Rice seedlings. Plant Physiol 95, 699-706. crossref(new window)

8.
Couee I, Sulmon C, Gouesbet G, and Amrani AE (2006) Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. J Exp Bot 57, 449-59. crossref(new window)

9.
DeVries AL (1986) Antifreeze glycopeptides and peptides: interactions with ice and water. Methods Enzymol 127, 293-303. crossref(new window)

10.
Fei SZ (2008) Perennial ryegrass anti-freeze protein genes enhances freezing tolerance in plants. Turfgrass Environ Research 28, 129-45.

11.
Gusta LV, Wisniewski M, Nesbitt NT, and Gusta ML (2004) The effect of water, sugars and proteins on the pattern of ice nucleation and propagation in acclimated and nonacclimated canola leaves. Plant Physiol 135, 1642-53. crossref(new window)

12.
Guy CL, Huber JLA, and Huber SC (1992) Sucrose phosphate synthase and sucrose accumulation at low temperature. Plant Physiol 27, 507-28.

13.
Hightower R, Baden C, Penzes E, Lund P, and Dunsmuir P (1991) Expression of antifreeze proteins in transgenic plants. Plant Mol Biol 17, 1013-21. crossref(new window)

14.
Hill LM, Morley-Smith ER, and Rawsthorne S (2003) Metabolism of sugars in the endosperm of developing seeds of oilseed rape. Plant Physiol 131, 228-36. crossref(new window)

15.
Hon WC, Griffith M, Mlynarz A, Kwok YC, and Yang DSC (1995) Antifreeze proteins in Winter Rye are similar to pathogenesis-related proteins. Plant Physiol 109, 879-89. crossref(new window)

16.
Jarillo JA, Leyva A, Salinas J, and Martinez-Zapater JM (1993) Low temperature induces the accumulation of alcohol dehydrogenase mRNA in Arabidopsis thaliana, a chilling-tolerant plant. Plant Physiol 101, 833-7.

17.
John UP, Polotnianka RM, Sivakumaran KA, Chew O, Mackin L, and Kuiper MJ (2009) Ice recrystallization inhibition proteins (IRIPs) and freeze tolerance in the cryophilic Antarctic hair grass Deschampsia antarctica E. Desv. Plant Cell Environ 32, 336-48. crossref(new window)

18.
Kankofer M (2001) Antioxidative defence mechanisms against reactive oxygen species in bovine retained and not-retained placenta: activity of glutathione peroxidase, glutathione transferase, catalase and superoxide dismutase. Placenta 22, 466-72. crossref(new window)

19.
Kaurin , Junttila O, and Hansen J (1981) Seasonal changes in frost hardiness in cloudberry (Rubus chamaemorus) in relation to carbohydrate content with special reference to sucrose. Physiol Plant 52, 310-4. crossref(new window)

20.
Klotke J, Kopka J, Gatzke N, and Heyer AG (2004) Impact of soluble sugar concentrations on the acquisition of freezing tolerance in accessions of Arabidopsis thaliana with contrasting cold adaptation-evidence for a role of raffinose in cold acclimation. Plant Cell Environ 27, 1395-404. crossref(new window)

21.
Knight CA and Duman JG (1986) Inhibition of recrystallization of ice by insect thermal hysteresis proteins: a possible cryoprotective role. Cryobiology 23, 256-62. crossref(new window)

22.
Liu J and Zhu JK (1997) Proline accumulation and salt-stress-induced gene expression in a salt-hypersensitive mutant of Arabidopsis. Plant Physiol 114, 591-6. crossref(new window)

23.
Livingston DP, Premakumar R, and Tallury SP (2006) Carbohydrate partitioning between upper and lower regions of the crown in oat and rye during cold acclimation and freezing. Cryobiology 52, 200-8. crossref(new window)

24.
Lv WT, Lin B, Zhang, and Hua XJ (2011) Proline accumulation is inhibitory to Arabidopsis seedlings during heat stress. Plant Physiol 156, 1921-33. crossref(new window)

25.
Meyer K, Keil M, and Naldrett MJ (1999) A leucine-rich repeat protein of carrot that exhibits antifreeze activity. FEBS Lett 447, 171-8. crossref(new window)

26.
Moffatt B, Ewart V, and Eastman A (2006) Cold comfort: plant antifreeze proteins. Physiol Plant 126, 5-16. crossref(new window)

27.
Molinari HBC, Marur CJ, Daros E, de Campos MKF, de Carvalho JFRP, Bespalhok JC et al. (2007) Evaluation of the stress-inducible production of proline in transgenic sugarcane (Saccharum spp.): osmotic adjustment, chlorophyll fluorescence and oxidative stress. Physiol Plant 130, 218-29. crossref(new window)

28.
Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15, 473-93. crossref(new window)

29.
Roh Kh, Kwak BK, Kim HU, Lee KR, Kim SH, Suh MC et al. (2011) Production of transgenic plants in Brassica napus winter cultivar 'Youngsan'. J Appl Biol Chem 54, 26-32. crossref(new window)

30.
Rolland F, Baena-Gonzalez E, and Sheen J (2006) Sugar sensing and signaling in plants: conserved and novel mechanisms. Annu Rev Plant Biol 57, 675-709. crossref(new window)

31.
Sakai A and Yoshida S (1968) The role of sugar and related compounds in variations of freezing resistance. Cryobiology 5, 160-74. crossref(new window)

32.
Sasaki H, Ichimura K, and Oda M (1996) Changes in sugar content during cold acclimation and deacclimation of Cabbage seedlings. Annal Bot 78, 365-9. crossref(new window)

33.
Strauss G and Hauser H (1986) Stabilization of lipid bilayer vesicles by sucrose during freezing. Proc NatI Acad Sci USA 83, 2422-6. crossref(new window)

34.
Takagi H (2008) Proline as a stress protectant in yeast: physiological functions, metabolic regulations, and biotechnological applications. Appl Microbiol Biotechnol 81, 211-23. crossref(new window)

35.
Thomashow MF (1999) Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. Annu Rev Plant Physiol Plant Mol Biol 50, 571-99. crossref(new window)

36.
Tremblay K, Ouellet F, Fournier J, Danyluk J, and Sarhan F (2005) Molecular characterization and origin of novel bipartite cold-regulated ice recrystallization inhibition proteins from cereals. Plant Cell Physiol 46, 884-91. crossref(new window)

37.
Xin Z and Browse J (1998) eskimo1 mutants of Arabidopsis are constitutively freezing-tolerant. Proc Natl Acad Sci USA 95, 7799-804. crossref(new window)

38.
Yemm EW and Willis AJ (1954) The estimation of Carbohydrates in plant extracts by anthrone. Biochem J 57, 508-14. crossref(new window)

39.
Zamecnik J and Janacek J (1992) Interaction of antifreeze proteins from coldhardened cereal seedlings with ice nucleation active bacteria. Cryobiology 29, 718-9.

40.
Zhu GY, Geuns JMC, Dussert S, Swennen R, and Panis B (2006) Change in sugar, sterol and fatty acid composition in banana meristems caused by sucrose-induced acclimation and its effects on cryopreservation. Physiol Plant 128, 80-94. crossref(new window)