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Development of Protein Biomarkers for the Authentication of Organic Rice
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 Title & Authors
Development of Protein Biomarkers for the Authentication of Organic Rice
Lee, Ju-Young; Lim, Jinkyu;
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The rice protein profiles of Oryza sativa L (Koshihikari) grown under organic and conventional cultivation regimes were compared on 2-D gels to develop diagnostic marker proteins for organic rice. The selected proteins, differentially expressed between organic and conventional rice, were compared with the differentially expressed proteins of another organic and conventional rice pairing, produced at a different location. In the first comparison among conventional, no-chemical, and organic rice grown in the same region, Korea, 13 proteins exhibiting differential expression in organic and conventionally grown plants were selected. Eight of the 13 proteins were down-regulated, and the 5 remaining proteins were up-regulated from conventional to organic rice. The second comparison pairing from Kyungju, revealed 12 differentially expressed proteins, with 8 down-regulated and 4 up-regulated proteins. Ten of the differentially expressed proteins that overlapped between the two comparison sets could not be clustered into any functional group using a functional annotation clustering tool. Further comparisons using another set of conventional and organic rice, belonging to a different variety of Oryza sativa L and produced in Sanchung, revealed 8 differentially expressed proteins, 5 of which were down-regulated and 3 of which were upregulated in the organic rice. Overall, 3 differentially expressed proteins were commonly found in all three organic rice crops. These 3 proteins, along with other overlapping differentially expressed proteins, can provide a good starting point for the development of signature proteins that can be used for the authentication of organic rice with a follow-up studies with more comparison sets.
2D gel;authentication;conventional;differential expression;endosperm;organic;Oryza sativa L;rice soluble protein;
 Cited by
Bourn D and Prescott J (2002) A comparison of the nutritional value, sensory qualities, and food safety of organically and conventionally produced foods. Critl Rev Food Sci Nutri 42, 1-34. crossref(new window)

Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248-54. crossref(new window)

Gammulla CG, Pascovici D, Atwell BJ, and Haynes PA (2011) Differential proteomic response of rice (Oryza sativa) leaves exposed to high- and low-temperature stress. Proteomics 11, 2839-50. crossref(new window)

Gao B, Lu Y, Sheng Y, Chen P, and Yu LL (2013) Differentiating organic and conventional sage by chromatographic and mass spectrometry flow injection fingerprints combined with principal component analysis. J Agric Food Chem 61, 2957-63. crossref(new window)

Guo M, Rupe MA, Zinselmeier C, Habben J, Bowen BA, and Smith OS (2004) Allelic variation of gene expression in maize hybrids. Plant Cell Physiol 16, 1707-16. crossref(new window)

Hamamoto K, Aki T, Shigyo M, Sato S, Ishida T, Yano K et al. (2012) Proteomic characterization of the greening process in rice seedlings using the MS spectral intensity-based label free method. J Proteome Res 11, 331-47. crossref(new window)

Han F, Chen H, Li XJ, Yang MF, Liu GS, and Shen SH (2009) A comparative proteomic analysis of rice seedlings under various high-temperature stresses. Biochim Biophys Acta 1794, 1625-34. crossref(new window)

Lee JY, Lee JW, Kim YR, Yeom YJ, and Lim J (2012) Proteomic changes in Odae polished white rice grown at different cultivation conditions. J Appl Biol Chem 55, 79-83. crossref(new window)

Lehesranta SJ, Koistinen KM, Massat N, Davies HV, Shapherd LV, McNicol JW et al. (2007) Effects of agricultural production systems and their components on protein profiles of potato tubers. Proteomics 7, 597-604. crossref(new window)

Liu CW, Hsu YK, Cheng YH, Yen HC, Wu YP, Wang CS et al. (2012) Proteomic analysis of salt-responsive ubiquitin-related proteins in rice roots. Rapid Comm Mass Spectrom 26, 1649-60. crossref(new window)

Lu C, Hawkesford MJ, Barraclough PB, Poulton PR, Wilson ID, Barker GL et al. (2005) Markedly different gene expression in wheat grown with organic or inorganic fertilizer. Proc Biol Sci 272, 1901-8. crossref(new window)

Miller L and Houghton JA (1945) The micro-Kjeldahl determination of teh nitrogen content of amino acids and proteins. J Biol Chem 159, 373-83.

Nawrocki A, Thorup-Kristensen K, and Jensen ON (2011) Quantitative proteomics by 2DE and MALDI MS/MS uncover the effects of organic and conventional cropping methods on vegetable products. J Proteomics 74, 2810-25. crossref(new window)

Novotna H, Kmiecik O, Galazka M, Krtkova V, Hurajova A, Schulzova V et al. (2012) Metabolomic fingerprinting employing DART-TOFMS for authentication of tomatoes and peppers from organic and conventional farming. Food Addit Contam Part A 29, 1335-46. crossref(new window)

Organic_Trade_Association (2015) U.S. organic showcased at biggest trade show in Korea, Korea.

Pandey A, Rajamani U, Verma J, Subba P, Charkraborty N, Datta A et al. (2010) Identification of extracellular matrix proteins of rice (Oryza sativa L.) involved in dehydration-responsive network: a proteomic approach. J Proteome Res 9, 3443-64. crossref(new window)

Peng Q, Tian R, Chen F, Li B, and Gao H (2015) Discrimination of producing area of Chinese Tongshan kaoliang spirit using electronic nose sensing characteristics combined with the chemometrics methods. Food Chem 178, 301-5. crossref(new window)

USDA (2015) Organic multi-ingredient foods, United States Department of Agriculture, USA.

Wang W, Chen LN, Wu H, Zang H, Gao S, Yang Y et al. (2013a) Comparative proteomic analysis of rice seedlings in response to inoculation with Bacillus cereus. Lett Appl Microbiol 56, 208-15. crossref(new window)

Wang Z, Chen P, Yu L, and Harrington Pde B (2013b) Authentication of organically and conventionally grown basils by gas chromatography/ mass spectrometry chemical profiles. Anal Chem 85, 2945-53. crossref(new window)

Yang P, Liang Y, Shen S, and Kuang T (2006) Proteome analysis of rice uppermost internodes at the milky stage. Proteomics 6, 3330-8. crossref(new window)

Zorb C, Betsche T, and Langenkamper G (2009) Search for diagnostic proteins to prove authenticity of organic wheat grains (Triticum aestivum L.). J Agric Food Chem 57, 2932-7. crossref(new window)