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Development of Duplex PCR Method for Simultaneous Detection of Rabbit (Oryctolagus cuniculus) and Cat (Felis catus) Meats
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 Title & Authors
Development of Duplex PCR Method for Simultaneous Detection of Rabbit (Oryctolagus cuniculus) and Cat (Felis catus) Meats
Hong, Yeun; Kim, Mi-Ju; Yang, Seung-Min; Yoo, In-Suk; Kim, Hae-Yeong;
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A duplex polymerase chain reaction (PCR) detection method was developed to authenticate the use of cat and rabbit in food and to prevent unlawful distribution of illegally butchered meat in both domestic and imported food market. Species-specific primers were designed targeting mitochondrial cytochrome b gene. The sizes of PCR products were 191 bp for cat and 101 bp for rabbit, which were relatively small for better application of the detection method on processed foods. Specificities of primers were verified using 21 animal species including cat and rabbit. Limit of detection was examined by serial dilution of the sample DNA and confirmed as 0.005 ng for rabbit and 0.0005 ng for cat using Bioanalyzer. The developed duplex PCR method showed specificity and sensitivity in the identification of two target species.
cat;duplex polymerase chain reaction;food authentication;multiplex polymerase chain reaction;rabbit;
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Ali ME, Razzak MA, Hamid SBA, Rahman MM, Amin MA, Rashid NRA et al. (2015) Multiplex PCR assay for the detection of five meat species forbidden in Islamic foods. Food Chem 177, 214-24. crossref(new window)

Amaral JS, Santos CG, Melo VS, Costa J, Oliveira MBPP, and Mafra I (2015) Identification of duck, partridge, pheasant quail, chicken and turkey meats by species-specific PCR assays to assess the authenticity of traditional game meat Alheira sausages. Food Control 47, 190-5. crossref(new window)

Arslan A, Ilhak OI, and Calicioglu M (2006) Effect of method of cooking on identification of heat processed beef using polymerase chain reaction (PCR) technique. Meat Sci 72, 326-30. crossref(new window)

Attardi G and Schatz G (1988) Biogenesis of mitochondria. Annu Rev Cell Biol 4, 289-331. crossref(new window)

Ghovvati S, Nassiri MR, Mirhoseini SZ, Moussavi, AH, and Javadmanech A (2009) Fraud identification in industrial meat products by multiplex PCR assay. Food Control 20, 696-9. crossref(new window)

Girish PS, Anjaneyulu ASR, Viswas KN, Anand M, Rajkumar N, and Shivakumar BM (2004) Sequence analysis of mitochondrial 12S rRNA gene can identify meat species. Meat Sci 66, 551-6. crossref(new window)

Haunshi S, Basumatary R, Girish PS, Doley S, Bardoloi RK, and Kumar A (2009) Identification of chicken, duck, pigeon and pig meat by speciesspecific markers of mitochondrial origin. Meat Sci 83, 454-9. crossref(new window)

Jonker KM, Tilburg JJHC, Hagele GH, and De Boer E (2008) Species identification in meat products using real-time PCR. Food Addit Contam: Part A 25, 527-33. crossref(new window)

Kim KH, Kim YS, Kim MR, Lee HY, Jung YK, Lee JH et al. (2014) Development of species-specific primer to determine the authenticity of vegetable raw materials in food. Food Eng Prog 18, 419-26. crossref(new window)

Koh BRD, Kim JY, Na HM, Park SD, and Kim YH (2011) Development of species-specific multiplex PCR assays of mitochondrial 12S rRNA and 16S rRNA for the identification of animal species. Kor J Vet Serv 34, 417-28. crossref(new window)

Li J, Hong Y, Kim JH, Qin P, Kim M, and Kim HY (2015) Multiplex-PCR for simultaneous identification of turkey, ostrich, chicken, and duck. J Korean Soc Appl Biol Chem, 58, 887-93. crossref(new window)

Park YC, Jung YH, Kim MR, Shin JH, Kim KH, Lee JH et al. (2013) Development of detection method for Niphon spinosus, Epinephelus bruneus, and Epinephelus septemfasciatus using 16S rRNA gene. Kor J Food Sci Technol 45, 1-7. crossref(new window)

Safdar M and Junejo Y (2015) A multiplex-conventional PCR assay for bovine, ovine, caprine and fish species identification in feedstuffs: Highly sensitive and specific. Food Control 50, 190-4. crossref(new window)

Walker JA, Hughes DA, Hedges DJ, Anders BA, Laborde ME, Shewale J et al. (2004) Quantitative PCR for DNA identification based on genomespecific interspersed repetitive elements. Genomics 83, 518-27. crossref(new window)

Yang L, Tan Z, Wang D, Xue, L, Guan MX, Huang T et al. (2014) Species identification through mitochondrial rRNA genetic analysis. Sci Reports 4, 4089, doi: 10.1038/srep04089. crossref(new window)

You J, Huang L, Zhuang J, and Mou Z (2014) Species-specific multiplex realtime PCR assay for identification of deer and common domestic animals. Food Sci Biotechnol 23, 133-9. crossref(new window)