JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Anti-inflammatory Activity of Extracts from Ultra-Fine Ground Saururus chinensis Leaves in Lipopolysaccharide-Stimulated Raw 264.7 Cells
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Anti-inflammatory Activity of Extracts from Ultra-Fine Ground Saururus chinensis Leaves in Lipopolysaccharide-Stimulated Raw 264.7 Cells
Kim, Dong-Hee; Cho, Jun-Hyo; Cho, Young-Je;
  PDF(new window)
 Abstract
Bioactive components of ultra-fine ground Saururus, the extraction yield increases when the leaves are ultra-fine ground. Comparison of normal-ground and ultra-fine ground Saururus chinensis leaves showed that the solid content and antiinflammatory activity of ultra-fine ground extracts was higher than that of normal-ground extracts. Lipopolysaccharide (LPS)-stimulated Raw 264.7 cells were treated with different concentrations of Saururus chinensis extract and the amount of nitric oxide (NO) was determined; LPS-treated cells produced 2 times more NO than cells that were not treated with LPS. Moreover, the NO production in cells treated with Saururus chinensis extract was inhibited in a concentration-dependent manner. Because the stimulant-induced NO production is regulated by the inducible nitric oxide synthase (iNOS), we measured the iNOS protein level to elucidate the mechanism by which the NO production was inhibited. We found that the amount of iNOS decreased dose-dependently. It was reduced by 53% at a Saururus chinensis extract concentration of . The protein expression of cyclooxygenase-2 (COX-2) in LPS-treated Raw 264.7 cells was inhibited by 31% at of Saururus chinensis extract. Gel shift of the nuclear factor kappa B-DNA complex occurred in LPS-treated cells and the intensity of the band decreased gradually in a concentration-dependent manner. Ultra-fine ground Saururus chinensis extract had a concentration-dependent inhibitory effect on the production of prostaglandin , tumor necrosis factor , interleukin (IL-), IL-6, and IL-8 in LPS-treated Raw 264.7 cells, i.e., at of Saururus chinensis extract, their levels were decreased by 53, 67, 52, 37, and 21% respectively.
 Keywords
anti-inflammation;ethanol extracts;Saururus chinensis;ultra-fine grinding;
 Language
English
 Cited by
1.
Physicochemical and in vitro digestion characteristics of size-different red ginseng powders, Food Science and Biotechnology, 2017, 2092-6456  crossref(new windwow)
 References
1.
A society for Korea medicinal botany (2001) In Medicinal botany, Hakchang publishing Co., Korea.

2.
Anfernee KT, Chi-Keung W, Xiao-Ling S, Mengsu Y, and Wang-Fun F (2005) Honokiol inhibits TNF-$\alpha$-stimulated NF-${\kappa}B$ activation and NF- ${\kappa}aB$-regulated gene expression through suppression of IKK activation. Biochem Pharm 70, 1443−57. crossref(new window)

3.
Byun SH, Yang CH, and Kim SC (2005) Inhibitory effect of Scrophulariae Radix extract on TNF-$\alpha$, IL-$1{\beta}$, IL-6 and nitric oxide production in lipopolysaccharide-activated Raw 264.7 cells. Kor J Herb 20, 7−16.

4.
Cheon YP, Mohammad LM, Park CH, Hong JH, Lee GD, Song JC et al. (2009) Bulnesia sarmienti aqueous extract inhibits inflammation in LPSstimulated Raw264.7 cells. J life science 19, 479−85. crossref(new window)

5.
Cho CW, Kim SW, Rho JH, Rhee YK, and Kim KT (2008) Extraction characteristics of saponin and acidic polysaccharide based on the red ginseng particle size. J Ginseng Res 32, 179−86. crossref(new window)

6.
Cho YJ and An BJ (2008) Anti-inflammatory effect of extracts from Cheongmoknosang (Morus alba L.) in lopopolysaccharide-stimulated Raw cells. J Kor Soc Appl Biol Chem 51, 44−8.

7.
Choe KH (1999) A study on chemical composition and anti-microbial activity of saururaceae growing in Korea. Ph. D. Thesis, Kyung Hee University, Korea.

8.
Choe KH, Yoon CH, and Kwon SJ (1994) A study on chemical composition of Saururaceac growing in Krean (5) on flavonoid constituents of Saururus chinensis. J Kor Soc anal Sci 7, 11−5.

9.
Gross SS and Wolin MS (1995) Nitric oxide: pathophysiological mechanisms. Annu Rev Physiol 57, 737−69. crossref(new window)

10.
Heo JC, Lee KY, Lee BG, Choi SY, Lee SH, and Lee SH (2010) Anti-allergic activities of ultra-fine powder from Persimmon. Kor J Food Preser 17, 145−50.

11.
Iwona M, Barbara M, Violetta RS, Romuald M, Zbigniew S, Maciej K et al. (2006) Proinflammatory cytokine (IL-$1{\beta}$, IL-6, IL-12, IL-18 and TNF-$\alpha$) levels in sera of patients with subacute cutaneous lupus erythematosus. Immunology Letters 102, 79−82. crossref(new window)

12.
Kim BH and Song WS (2000) The dyeability and antimicrobial activity of Saururus chinensis (I). J Kor I lomc Economics 38, 1-9.

13.
Kim JY, Park SJ, Yun KJ, Cho YW, Park HJ, and Lee KT (2008) Isoliquritigenin isolated from the roots of Glycyrrhiza uralensis inhibits LPS-induced iNOS and COX-2 expression via the attenuation of NF-${\kappa}B$ in Raw264.7 macrophages. Euro J phamacol 584, 175-84. crossref(new window)

14.
Kim NK, Kim MH, Yoon CS, and Choi SW (2006) Studies on the antiinflammatory activity of Paulownia coreana Uyeki leaf extracts. J Soc Cosmet Scie Korea 32, 241-7.

15.
Kim SK, Ban SY, Kim JS, and Chung SK (2005) Change of antioxidant activity and antioxidant compounds in Saururus chinensis by extraction conditions. J Kor Soc Appl Biol Chem 48, 89−92.

16.
Kroncke KD, Fehsel K, and Kolb-Bachofen V (1998) Inducible nitric oxide synthase in human diseases. Clin Exp Immunol 113, 147−56. crossref(new window)

17.
Kwak JW (1988) Pharmacological studies on Saururus chinensis Bail. MS Thesis, Kyung Hee university, Korea.

18.
Kwon SA (1999a) A second series about Saururus chinensis and Houttuynia cordata Thunberg. J Kor Oriental Drug 2, 18−20.

19.
Kwon SA (1999b) A second series about Saururus chinensis and Houttuynia cordata Thunberg. J Kor Oriental Drug 3, 28−31.

20.
Kwon SH (1996) The isolation of anti-hepatotoxic constituents from Saururus chinensis. MS Thesis, Seoul national university, Korea .

21.
Lee ST, Lee YH, Choi YJ, Lee YH, Choi JS, and Heo JS (2001) Yield and bioactive component on different compostamounts and culture method of Saururus chinensis Baill. Kor J Med Crop Sci 9, 220−4.

22.
Lee ST, Park JM, Lee HK, Kim MB, Cho JS, and Heo JS (2000) Component comparison in different growth stages and organs of Saururus chinensis Bail. Kor J Med Crop Sci 8. 312−8.

23.
Lee YN (2002) In Flora of Korea. Kyo-Hak Publishing Co. Ltd., Korea.

24.
Lim DK, Choi U, and Shin DH (1996) Antioxidative activity of ethanol extract from Korean medicinal plants. Kor J Food Sci Technol 28, 83−9.

25.
Liu YP, Wen JK, Wu YB, Zhang J, Zheng B, Zhang DQ et al. (2009) 1,6- O,O-diacetylbritannilactones inhibits IeB kinase $\beta$-dependent NF-${\kappa}B$ activation. Phytomedicine 16, 156−60. crossref(new window)

26.
Naoko K, Satsuki K, and Shinichi W (2005) IL-17 suppresses TNF-$\alpha$- induced CCL27 production through induction of COX-2 in human keratinocytes. J Aller Clin Immun 116, 1144−50. crossref(new window)

27.
Nirupama S, Anil KS, Battu A, Sangita M, Sudip G, and Nasreen ZE (2005) Human resistin stimulates the pro-inflammatory cytokines TNF-asand IL-12 in macrophages by NF-${\kappa}B$-dependent pathway. Biochem and Biophy Res Comm 334, 1092−101. crossref(new window)

28.
Oshima H and Bartsch H (1994) Chronic infections and inflammatory processes as cancer risk factors: possible role of nitric oxide in carcinogenesis. Mutat Res 305, 253−64. crossref(new window)

29.
Park JH and Lee CK (2000) In The encyclopedia of medicinal plants. Shinilbook Publishing Co., Korea.

30.
Park SJ, Kim JY, Jang YP, Cho YW, Ahn EM, Baek NI et al. (2007) Inhibition of LPS induced iNOS, COX-2 and cytokines expression by genistein-4'-O-$\alpha$-L-rhamnopyranosyl-(1-2)-$\beta$-D-glucopyranoside through the NF-${\kappa}B$ inactivation in Raw 264.7 Cells. Kor J Pharmacogn 38, 339−48.

31.
Park SJ, Shin JS, Cho W, Cho YW, Ahn EM, Baek NI et al. (2008) Inhibition of LPS induced iNOS, COX-2 and cytokines expression by kaempferol- 3-O-$\beta$-D-sophoroside through the NF-${\beta}B$ inactivation in RAW 264.7 cells. Kor J Pharmacogn 39, 95−103.

32.
Park SS, Yu KH, and Min TJ (1998) Antioxidative activity of ethanol extract from fruiting bodies of mushrooms. Kor J Mycol 26, 69−77.

33.
So MS, Lee JS, and Yi SY (2004) Inhibition of nitric oxide and cytokines in Macrophages by Codonopsis lanceolata. Kor J Food Sci Technol 36, 986−90.

34.
Stuehr DJ (1999) Mammalian nitric oxide synthase. Biochem Biophys Acta 1411, 217−30.

35.
Syu-ichi K, Ai S, Ayako T, Takako H, Yu O, Mayuko U et al. (2005) Inhibitory effect of naringin on lipopolysaccharide (LPS)-induced endotoxin shock in mice and nitric oxide production in Raw 264.7 macrophages. Life Sciences 25, 1−9.

36.
Tolksdorf S, McCready M, McCullagh D, and Schwenk E (1949) The Turbidimetric Assay of Hyaluronidase. J Lab Clin Med 34, 74−81

37.
Won SJ, Park HJ, and Lee KT (2008) Inhibition of LPS induced iNOS, COX- 2 and cytokines expression by salidroside through the NF-${\kappa}B$ inactivation in RAW 264.7 cells. Kor J Pharmacogn 39, 110−7.

38.
Yoon WJ, Lee JA, Kim KN, Kim JY, and Park SY (2007) In vitro antiinflammatory activity of the Artemisia fukudo extracts in murine macrophage Raw264.7 cells. Kor J Food Sci Technol 39, 464−9.