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Effects of Temperature on Systemic Infection and Symptom Expression of Turnip mosaic virus in Chinese cabbage (Brassica campestris)
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  • Journal title : The Plant Pathology Journal
  • Volume 31, Issue 4,  2015, pp.363-370
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/PPJ.NT.06.2015.0107
 Title & Authors
Effects of Temperature on Systemic Infection and Symptom Expression of Turnip mosaic virus in Chinese cabbage (Brassica campestris)
Chung, Bong Nam; Choi, Kyung San; Ahn, Jeong Joon; Joa, Jae Ho; Do, Ki Seck; Park, Kyo-Sun;
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Using the Chinese cabbage (Brassica campestris) cultivar 'Chun-goang' as a host and turnip mosaic virus (TuMV) as a pathogen, we studied the effects of ambient temperature (, , , and ) on disease intensity and the speed of systemic infection. The optimal temperature for symptom expression of TuMV was . However, symptoms of viral infection were initiated at and 6 days post infection (dpi). Plants maintained at were systemically infected as early as 6 dpi and remained symptomless until 12 or 22 dpi, depending on growth stage at the time of inoculation. It took 45 days for infection of plants grown at . Quantitative realtime polymerase chain reaction (q-PCR) results showed that the accumulation of virus coat protein was greater in plants grown at . The speed of systemic infection increased linearly with rising ambient temperature, up to . The zero-infection temperature was . To study the effects of abruptly elevated temperatures on systemic infection, plants inoculated with TuMV were maintained at for 20 d; transferred to a growth chamber at temperatures of , , , , or for 1, 2, or 3 d; and then moved back to . The numbers of plants infected increased as duration of exposure to higher temperatures and dpi increased.
chinese cabbage;disease symptoms;temperature;TuMV;
 Cited by
Viruses infecting Brassica crops in the Black Sea Region of Turkey, Acta Agriculturae Scandinavica, Section B — Soil & Plant Science, 2016, 66, 7, 553  crossref(new windwow)
Chellappan, P., Vanitharani, R., Ogbe, F. and Fauquet, C. M. 2005. Effect of temperature on geminivirus-induced RNA Silencing in Plants. Plant Physiol. 138:1828-1841. crossref(new window)

Chivasa, S., Ekpo, E. J. A. and Hicks, R. G. T. 2001. New hosts of Turnip mosaic virus in Zimbabwe. New Dis. Rep. 4:5.

Cho, J. D., Choi, H. S., Kim, J. S., Kim, K. H. and Kim, K. S. 2003. Symptom variances in mixed infections of six turnip mosaic virus and one ribgrass mosaic virus isolates in crucifers. Plant Pathol. J. 19:111-116. crossref(new window)

Choi, J. K. 1995. Annual report. Ministry of Agriculture, Forestry and Fisheries.

Chu, F.-L. E. and Volety, A. K. 1997. Disease processes of the parasite Perkinsus marinus in eastern oyster Crassostrea virginica: minimum dose for infection initiation, and interaction of temperature, salinity and infective cell dose. Dis. Aquat. Organ. 28:61-68. crossref(new window)

Fajinmi, A. A. and Fajinmi, O. B. 2010. Incidence of okra mosaic virus at different growth stages of okra plants (Abelmoschus esculentus (L.) Moench) under tropical condition. J. Gen. Mol. Virol. 2:28-3.

Feil, H. and Purcell, A. H. 2001. Temperature-dependent growth and survival of Xylella fastidiosa in vitro and in potted grapevines. Plant Dis. 85:1230-1234. crossref(new window)

Ha, C., Revill, P., Harding, R. M., Vu, M. and Dale, J. L. 2008. Identification and sequence analysis of potyviruses infecting crops in Vietnam. Arch. Virol. 153:45-60. crossref(new window)

Jandel Scientific. 1996. TableCurve 2D. Automated curve fitting and equation discovery: Version 4.0. Jandel Scientific, Sam Rafael, CA, USA.

Jones, R. W., Jackson, A. O. and Morris, T. J. 1990. Defective-interfering RNAs and elevated temperatures inhibit replication of tomato bushy stunt virus in inoculated protoplasts. Virology 176:539-545. crossref(new window)

Kido, K., Tanaka, C., Mochizuki, T., Kubota, K., Ohki, T., Ohnishi, J., Knight, L. M. and Tsuda, S. 2008. High temperatures activate local viral multiplication and cell-to-cell movement of Melon necrotic spot virus but restrict expression of systemic symptoms. Phytopathology 98:181-186. crossref(new window)

Leisner, S. M., Turgeon, R. and Howell, S. H. 1992. Long distance movement of califlower mosaic virus infected turnip plant. Mol. Plant-Microbe Interact. 5:41-47. crossref(new window)

Nguyen, H. D., Tomitaka, Y., Ho, S. Y. M., Duchene, S., Vetten, H. J., Lesemann, D., Walsh, J. A., Gibbs, A. J. and Ohshima, K. 2013. Turnip mosaic potyvirus probably first spread to eurasian brassica crops from wild orchids about 1000 years Ago. PLoS One 8:e55336. crossref(new window)

Ohshima, K., Yamaguchi, Y., Hirota, R., Hamamoto, T., Tomimura, K., Tan, Z., Sano, T., Azuhata, F., Walsh, J. A., Fletcher, J., Chen, J., Gera, A. and Gibbs, A. J. 2002. Molecular evolution of Turnip mosaic virus : evidence of host adaptation, genetic recombination and geographical spread. J. Gen. Virol. 83:1511-1521. crossref(new window)

Provvidenti, R. 1981. Turnip mosaic virus. Plant Viruses Online. Descriptions and Lists from the VIDE Database.

Soler, S., Diez, M. J. and Nuez, F. 1998. Effect of temperature regime and growth stage interaction on pattern of virus presence in TSWV-resistant accessions of Capsicum chinense. Plant Dis. 82:1199-1204. crossref(new window)

Spence, N. J. 1999. Survey of viruses of vegetable crops in the peri-urban production system of Kenya. Final Technical Report for DFID CPP Project ZA0272.

Spence, N. J., Phiri, N. A., Hughes, S. L., Mwaniki, A., Simons, S., Oduor, G., Chacha, D., Kuria, A., Ndirangu, S., Kibata, G. N. and Marris, G. C. 2007. Economic impact of Turnip mosaic virus, Cauliflower mosaic virus and Beet mosaic virus in three Kenyan vegetables. Plant Physiol. 56:317-323.

Szittya, G., Silhavy, D., Molnar, A., Havelda, Z., Lovas, A., Lakatos, L., Banfalvi, Z. and Burgyan, J. 2003. Low temperature inhibits RNA silencing-mediated defence by the control of siRNA generation. EMBO J. 22:633-640. crossref(new window)

Tamada, T. and Harrison, B. D. 1981. Quantitative studies on the uptake and retention of potato leafroll virus by aphids in laboratory and field conditions Ann. Appl. Biol. 98:261-276. crossref(new window)

Tomlinson, J. A. 1970. Turnip mosaic virus. Description of plant viruses. Association of Applied Biologists.

Voinnet, O. 2001. RNA silencing as a plant immune system against viruses. Trends Genet. 17:449-459. crossref(new window)

Wagner, T. L., Wu, H., Sharpe, P. J. H., Schoolfield, R. M. and Coulson, R. N. 1984. Modeling insect development rates: a literature review and application of a biophysical model. Ann. Entomol. Soc. Am. 77:208-225. crossref(new window)

Walsh, J. A. and Jenner, C. E. 2002. Turnip mosaic virus and the quest for durable resistance. Mol. Plant Pathol. 3:289-300. crossref(new window)

Yoon, M. K., Kim, J. Y., Choi, G. S. and Kim, J. S. 1995. Soil transtnission, screening of resistant variety and incidence of Ribgrass mosaic virus occurring in Chinese cabbage in autumn growing season Kor. J. Plant Pathol. 11:191 (Abst.).

Zhang, X., Zhang, X., Singh, J., Li, D. and Qua, F. 2012. Temperature-dependent survival of Turnip crinkle virus-infected Arabidopsis plants relies on an RNA silencing-based defense that requires DCL2, AGO2, and HEN1. J. Virol. 12:6847-6854.

Zitter, T. A. and Murphy, J. F. 2009. Cucumber mosaic. The Plant Health Instructor. DOI: 10.1094/PHI-I-2009-0518-01. crossref(new window)