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Loop-mediated Isothermal Amplification Assay to Rapidly Detect Wheat Streak Mosaic Virus in Quarantined Plants
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  • Journal title : The Plant Pathology Journal
  • Volume 31, Issue 4,  2015, pp.438-440
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/PPJ.NT.06.2015.0110
 Title & Authors
Loop-mediated Isothermal Amplification Assay to Rapidly Detect Wheat Streak Mosaic Virus in Quarantined Plants
Lee, Siwon; Kim, Jin-Ho; Choi, Ji-Young; Jang, Won-Cheoul;
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 Abstract
We developed a loop-mediated isothermal amplification (LAMP) method to rapidly diagnose Wheat streak mosaic virus (WSMV) during quarantine inspections of imported wheat, corn, oats, and millet. The LAMP method was developed as a plant quarantine inspection method for the first time, and its simplicity, quickness, specificity and sensitivity were verified compared to current reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR quarantine methods. We were able to quickly screen for WSMV at quarantine sites with many test samples; thus, this method is expected to contribute to plant quarantine inspections.
 Keywords
LAMP;loop-mediated isothermal amplification;quarantine;wheat streak mosaic virus;WSMV;
 Language
English
 Cited by
 References
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