Loop-mediated Isothermal Amplification Assay to Rapidly Detect Wheat Streak Mosaic Virus in Quarantined Plants Lee, Siwon; Kim, Jin-Ho; Choi, Ji-Young; Jang, Won-Cheoul;
We developed a loop-mediated isothermal amplification (LAMP) method to rapidly diagnose Wheat streak mosaic virus (WSMV) during quarantine inspections of imported wheat, corn, oats, and millet. The LAMP method was developed as a plant quarantine inspection method for the first time, and its simplicity, quickness, specificity and sensitivity were verified compared to current reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR quarantine methods. We were able to quickly screen for WSMV at quarantine sites with many test samples; thus, this method is expected to contribute to plant quarantine inspections.
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Lee, S., Kang, E. H., Heo, N. Y., Kim, S. M., Kim, Y. J. and Shin, Y. G. 2013b. Detection of Carnation necrotic fleck vrus and Carnation ringspot virus using RT-PCR. Res. Plant Dis. 19:36-44.
Lee, S., Kang, E. H., Shin, Y. G. and Lee, S. H. 2013c. Development of RT-PCR and nested PCR for detection of four quarantine plant viruses belonging to Nepovirus. Res. Plant Dis. 19:220-225.
Lee, S., Lee, J. Y., Shin, Y. G., Lee, S. H. and Ahn, T. Y. 2015. Development and verification of nested PCR assay for detection of Tobacco rattle virus in plant quarantine. J. Bacteriol. Virol. 45:54-61.
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