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Lysimachia foenum-graecum Herba Extract, a Novel Biopesticide, Inhibits ABC Transporter Genes and Mycelial Growth of Magnaporthe oryzae
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  • Journal title : The Plant Pathology Journal
  • Volume 32, Issue 1,  2016, pp.8-15
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/PPJ.OA.08.2015.0157
 Title & Authors
Lysimachia foenum-graecum Herba Extract, a Novel Biopesticide, Inhibits ABC Transporter Genes and Mycelial Growth of Magnaporthe oryzae
Lee, Youngjin;
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To identify a novel biopesticide controlling rice blast disease caused by Magnaporthe oryzae, 700 plant extracts were evaluated for their inhibitory effects on mycelial growth of M. oryzae. The L. foenum-graecum Herba extract showed the lowest inhibition concentration () of , which is lower than the of blasticidin S (), a conventional fungicide for rice blast disease. When treatments were combined, the of blasticidin S was dramatically reduced to . Since ABC transporter genes are involved in fungicide resistance of many organisms, we performed RT-PCR to investigate the transcriptional changes of 40 ABC transporter family genes of M. oryzae treated with the plant extract, blasticidin S, and tetrandrine, a recognized ABC transporter inhibitor. Four ABC transporter genes were prominently activated by blasticidin S treatment, but were suppressed by combinational treatment of blasticidin S with the plant extract, or with tetrandrine that didn`t show cellular toxicity by itself in this study. Mycelial death was detected via confocal microscopy at 24 h after plant extract treatment. Finally, subsequent rice field study revealed that the plant extract had high control efficacy of 63.3% and should be considered a biopesticide for rice blast disease. These results showed that extract of L. foenum graecum Herba suppresses M. oryzae ABC transporter genes inducing mycelial death and therefore may be a potent novel biopesticide.
ABC transporter gene;biopesticide;blasticidin S;fungicide resistance;Lysimachia foenum-graecum Herba;Magnaporthe oryzae;plant extract;rice blast disease;
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