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Development of Specific Markers for Identification of Biovars 1 and 2 Strains of Pseudomonas syringae pv. actinidiae
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  • Journal title : The Plant Pathology Journal
  • Volume 32, Issue 2,  2016, pp.162-167
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/PPJ.NT.10.2015.0224
 Title & Authors
Development of Specific Markers for Identification of Biovars 1 and 2 Strains of Pseudomonas syringae pv. actinidiae
Lee, Young Sun; Kim, Gyoung Hee; Koh, Young Jin; Zhuang, Qiguo; Jung, Jae Sung;
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 Abstract
Pseudomonas syringae pv. actinidiae, the causal agent of canker in kiwifruit, can be divided into three biovars (biovars 1, 2, and 3). Strains belonging to biovar 1 produce phaseolotoxin and were isolated in Japan and Italy before 2008. Strains of biovar 2 produce coronatine instead of phaseolotoxin and have been isolated only in Korea. Strains belonging to biovar 3 produce neither phaseolotoxin nor coronatine and are responsible for the global outbreak of bacterial canker of kiwifruit in recent years. The biovar 3-specific primer set was developed in a previous work. In this study, two sets of PCR primers specific to strains of biovars 1 and 2, respectively, were developed based on random amplified polymorphic DNA analyses. Primers PsaJ-F and PsaJ-R produced a 481-bp region with genomic DNA of biovar 1 strains, whereas primers PsaK-F and PsaK-R amplified a 413-bp region present only in the genome of biovar 2 strains.
 Keywords
bacterial canker;biovar;kiwifruit;molecular marker;Pseudomonas syringae pv. actinidiae;
 Language
English
 Cited by
 References
1.
Balestra, G. M., Renzi, M. and Mazzaglia, A. 2010. First report of bacterial canker of Actinidia deliciosa caused by Pseudomonas syringae pv. actinidiae in Portugal. New Dis. Rep. 22:10. crossref(new window)

2.
Balestra, G. M., Renzi, M. and Mazzaglia, A. 2011. First report of Pseudomonas syringae pv. actinidiae on kiwifruit plants in Spain. New Dis. Rep. 24:10. crossref(new window)

3.
Balestra, G. M., Taratufolo, M. C., Vinatzer, B. A. and Mazzaglia, A. 2013. A multiplex PCR assay for detection of Pseudomonas syringae pv. actinidiae and differentiation of populations with different geographic origin. Plant Dis. 97:472-478. crossref(new window)

4.
Bastas, K. and Karakaya, A. 2012. First report of bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae in Turkey. Plant Dis. 96:452.

5.
Bereswill, S., Bugert, P., Volksch, B., Ullrich, M., Bender, C. L. and Geider, K. 1994. Identification and relatedness of coronatine-producing Pseudomonas syringae pathovars by PCR analysis and sequence determination of the amplification products. Appl. Environ. Microbiol. 60:2924-2930.

6.
Chapman, J. R., Taylor, R. K., Weir, B. S., Romberg, M. K., Vanneste, J. L., Luck, J. and Alexander, B. J. R. 2012. Phylogenetic relationships among global populations of Pseudomonas syringae pv. actinidiae. Phytopathology 102:1034-1044. crossref(new window)

7.
Cunty, A., Poliakoff, F., Rivoal, C., Cesbron, S., Fischer-Le Saux, M., Lemaire, C., Jacques, M. A., Manceau, C. and Vanneste, J. L. 2015. Characterization of Pseudomonas syringae pv. actinidiae (Psa) isolated from France and assignment of Psa biovar 4 to a de novo pathovar: Pseudomonas syringae pv. actinidifoliorum pv. nov. Plant Pathol. 64:582-596. crossref(new window)

8.
European Plant Protection Organization, 2011. First report of Pseudomonas syringae pv. actinidiae in Chile. EPPO Reporting Service 3:55.

9.
Everett, K. R., Taylor, R. K., Romberg, M. K., Rees-George, J., Fullerton, R. A., Vanneste, J. L. and Manning, M. A. 2011. First report of Pseudomonas syringae pv. actinidiae causing kiwifruit bacterial canker in New Zealand. Australas. Plant Dis. Notes 6:67-71. crossref(new window)

10.
Ferrante, P. and Scortichini, M. 2009. Identification of Pseudomonas syringae pv. actinidiae as causal agent of bacterial canker of yellow kiwifruit (Actinidia chinensis Planchon) in central Italy. J. Phytopathol. 157:768-770. crossref(new window)

11.
Ferrante, P. and Scortichini, M. 2010. Molecular and phenotypic features of Pseudomonas syringae pv. actinidiae isolated during recent epidemics of bacterial canker on kiwifruit (Actinidia chinensis) in central Italy. Plant Pathol. 59:954-962. crossref(new window)

12.
Ferrante, P. and Scortichini, M. 2015. Redefining the global populations of Pseudomonas syringae pv. actinidiae based on pathogenic, molecular and phenotypic characteristics. Plant Pathol. 64:51-62. crossref(new window)

13.
Han, H. S., Koh, Y. J., Hur, J.-S. and Jung, J. S. 2003. Identification and characterization of coronatine-producing Pseudomonas syringae pv. actinidiae. J. Microbiol. Biotechnol. 13:110-118.

14.
Koh, H. S., Kim, G. H., Lee, Y. S., Koh, Y. J. and Jung, J. S. 2014. Molecular characteristics of Pseudomonas syringae pv. actinidiae strains isolated in Korea and a multiplex PCR assay for haplotype differentiation. Plant Pathol. J. 30:96-101. crossref(new window)

15.
Koh, Y. J., Cha, B. J., Chung, H. J. and Lee, D. H. 1994. Outbreak and spread of bacterial canker in kiwifruit. Kor. J. Plant Pathol. 10:68-72.

16.
Koh, Y. J., Kim, G. H., Jung, J. S., Lee, Y. S. and Hur, J. S. 2010. Outbreak of bacterial canker on Hort16A (Actinidia chinensis Planchon) caused by Pseudomonas syringae pv. actinidiae in Korea. N. Z. J. Crop Hortic. Sci. 38:275-282. crossref(new window)

17.
Koh, Y. J., Kim, G. H., Koh, H. S., Lee, Y. S., Kim, S.-C. and Jung, J. S. 2012. Occurrence of a new type of Pseudomonas syringae pv. actinidiae strain of bacterial canker on kiwifruit in Korea. Plant Pathol. J. 28:423-427. crossref(new window)

18.
Liang, Y., Zhang, X., Tian, C., Gao, A. and Wang, P. 2000. Pathogenic identification of kiwifruit bacterial canker in Shaanxi. J. Northwest Forestry College 15:37-39.

19.
Mazzaglia, A., Renzi, M. and Balestra, G. M. 2011. Comparison and utilization of different PCR-based approaches for molecular typing of Pseudomonas syringae pv. actinidiae strains from Italy. Can. J. Plant Pathol. 33:8-18. crossref(new window)

20.
Mazzaglia, A., Studholme, D. J., Taratufolo, M. C., Cai, R., Almeida, N. F., Goodman, T., Guttman, D. S., Vinatzer, B. A. and Balestra, G. M. 2012. Pseudomonas syringae pv. actinidiae (PSA) isolates from recent bacterial canker of kiwifruit outbreaks belong to the same genetic lineage. PLoS One 7:e36518. crossref(new window)

21.
McCann, H. C., Rikkerink, E. H. A., Bertels, F., Fiers, M., Lu, A., Rees-George, J., Andersen, M. T., Gleave, A. P., Haubold, B., Wohlers, M. W., Guttman, D. S., Wang, P. W., Straub, C., Vanneste, J., Rainey, P. B. and Templeton, M. D. 2013. Genomic analysis of the kiwifruit pathogen Pseudomonas syringae pv. actinidiae provides insights into the origins of an emergent plant disease. PLoS Pathogens 9: e1003503. crossref(new window)

22.
Rees-George, J., Vanneste, J. L., Cornish, D. A., Pushparajah, I. P. S., Yu, J., Templeton, M. D. and Everett, K. R. 2010. Detection of Pseudomonas syringae pv. actinidiae using polymerase chain reaction (PCR) primers based on the 16S-23S rDNA intertranscribed spacer region and comparison with PCR primers based on other gene regions. Plant Pathol. 59:453-464. crossref(new window)

23.
Sawada, H., Takeuchi, T. and Matsuda, I. 1997. Comparative analysis of Pseudomonas syringae pv. actinidiae and pv. phaseolicola based on phaseolotoxin-resistant ornitine carbamoyltransferase gene (argK) and 16S-23S rRNA intergenic spacer sequences. Appl. Environ. Microbiol. 63:282-288.

24.
Scotichini, M. 1994. Occurrence of Pseudomonas syringae pv. actinidiae on kiwifruit in Italy. Plant Pathol. 43:1035-1038. crossref(new window)

25.
Scotichini, M., Marcelletti, S., Ferrante, P., Petriccione, M. and Firrao, G. 2012. Pseudomonas syringae pv. actinidiae: a reemerging, multi-faceted, pandemic pathogen. Mol. Plant Pathol. 13:631-640. crossref(new window)

26.
Takikawa, Y., Serizawa, S., Ichikawa, T., Tsuyumu, S. and Goto, M. 1989. Pseudomonas syringae pv. actinidiae sp. nov., the causal bacterium of canker in kiwifruit in Japan. Ann. Phytopathol. Soc. Japan 55:437-444. crossref(new window)

27.
Untergasser, A., Cutcutache, I., Koressaar, T., Ye, J., Faircloth, B., Remm, M. and Rozen, S. 2012. Primer3-new capabilities and interfaces. Nucleic Acids Res. 40:e115. crossref(new window)

28.
Vanneste, J. L., Poliakoff, F., Audusseau, C., Cornish, D. A., Pailard, S., Rivoal, C. and Yu, J. 2011. First report of Pseudomonas syringae pv. actinidiae, the causal agent of bacterial canker of kiwifruit in France. Plant Dis. 95:1311.

29.
Young, J. M. 2012. Pseudomonas syringae pv. actinidiae in New Zealand. J. Plant Pathol. 94:S1.5-S1.10.