Advanced SearchSearch Tips
Natural Occurrence of Tomato leaf curl New Delhi virus in Iranian Cucurbit Crops
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : The Plant Pathology Journal
  • Volume 32, Issue 3,  2016, pp.201-208
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/PPJ.OA.10.2015.0210
 Title & Authors
Natural Occurrence of Tomato leaf curl New Delhi virus in Iranian Cucurbit Crops
Yazdani-Khameneh, Sara; Aboutorabi, Samaneh; Shoori, Majid; Aghazadeh, Azin; Jahanshahi, Parastoo; Golnaraghi, Alireza; Maleki, Mojdeh;
  PDF(new window)
The main areas for field-grown vegetable production in Iran were surveyed during the years of 2012-2014 to determine the occurrence of begomoviruses infecting these crops. A total of 787 leaf samples were collected from vegetables and some other host plants showing virus-like symptoms and tested by an enzymelinked immunosorbent assay (ELISA) using polyclonal antibodies produced against Tomato yellow leaf curl virus (TYLCV). According to the ELISA results, 81 samples (10.3%) positively reacted with the virus antibodies. Begomovirus infections were confirmed by polymerase chain reaction (PCR) using previously described TYLCV-specific primer pair TYLCV-Sar/TYLCV-Isr or universal primer pair Begomo-F/Begomo-R. The PCR tests using the primer pair TYLCV-Sar/TYLCV-Isr resulted in the amplification of the expected fragments of ca. 0.67-kb in size for ELISA-positive samples tested from alfalfa, pepper, spinach and tomato plants, confirming the presence of TYLCV. For one melon sample, having a week reaction in ELISA and no reaction in PCR using TYLCV-specific primers, the PCR reaction using the primer pair Begomo-F/Begomo-R resulted in the amplification fragments of the expected size of ca. 2.8 kb. The nucleotide sequences of the DNA amplicons derived from the isolate, Kz-Me198, were determined and compared with other sequences available in GenBank. BLASTN analysis confirmed the begomovirus infection of the sample and showed 99% identities with Tomato leaf curl New Delhi virus (ToLCNDV); phylogenetic analysis supported the results of the database searches. This study reports the natural occurrence of TYLCV in different hosts in Iran. Our results also reveal the emergence of ToLCNDV in Iranian cucurbit crops.
Begomovirus;phylogeny;Tomato leaf curl New Delhi virus;Tomato yellow leaf curl virus;vegetables;
 Cited by
The Prediction of a New CLCuD Epidemic in the Old World, Frontiers in Microbiology, 2017, 8  crossref(new windwow)
Tomato Leaf Curl New Delhi Virus: An Emerging Virus Complex Threatening Vegetable and Fiber Crops, Viruses, 2017, 9, 10, 264  crossref(new windwow)
Akhter, A., Qazi, J., Saeed, M. and Mansoor, S. 2009. A severe leaf curl disease on chillies in Pakistan is associated with multiple begomovirus components. Plant Dis. 93:962.

Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, Z., Miller, W. and Lipman, D. J. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl. Acids Res. 25:3389-3402. crossref(new window)

Bananej, K. and Vahdat, A. 2008. Identification, distribution and incidence of viruses in field-grown cucurbit crops of Iran. Phytopathol. Mediterr. 47:247-257.

Bananej, K., Vahdat, A. and Hosseini-Salekdeh, G. 2009. Begomoviruses associated with yellow leaf curl disease of tomato in Iran. J. Phytopathol. 157:243-247. crossref(new window)

Briddon, R. W. 2015. Geminiviridae. eLS Online Publication. doi: 10.1002/ 9780470015902.a0000750.pub3. crossref(new window)

Brown, J. K. and Bird, J. 1992. Whitefly-transmitted geminiviruses and associated disorders in the Americas and the Caribbean Basin: past and present. Plant Dis. 76:220-225. crossref(new window)

Brunt, A. A., Crabtree, K., Dallwitz, M. J., Gibbs, A. J. and Watson, L. 1995. Viruses of plants: description and lists from the VIDE database. CAB International, Wallingford, UK.

Clark, M. F. and Adams, A. M. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for detection of plant viruses. J. Gen. Virol. 34:475-483. crossref(new window)

Dellaporta, S. L., Wood, J. and Hicks, J. B. 1983. A plant DNA minipreparation version II. Plant Mol. Biol. Report. 1:19-21. crossref(new window)

Dias, J. S. 2011. World importance, marketing and trading of vegetables. Acta Hortic. 921:153-169.

Efron, B., Halloran, E. and Holmes, S. 1996. Bootstrap confidence levels for phylogenetic trees. Proc. Natl. Acad. Sci. U. S. A. 93:7085-7090. crossref(new window)

Food and Agriculture Organization of the United Nations (2012 onwards). FAOSTAT database results. URL [2 April 2015].

Farzadfar, Sh., Pourrahim, R., Golnaraghi, A. R. and Ahoonmanesh, A. 2005. Occurrence of Cauliflower mosaic virus in different cruciferous plants in Iran. Plant Pathol. 54:810. crossref(new window)

Fazeli, R., Heydarnejad, J., Massumi, H., Shaabanian, M. and Varsani, A. 2009. Genetic diversity and distribution of tomato-infecting begomoviruses in Iran. Virus Genes 38:311-319. crossref(new window)

Golnaraghi, A. R., Hamedi, A., Yazdani-Khameneh, S. and Khosroshahi, T. S. 2013. First report of a natural occurrence of Tomato yellow fruit ring virus on pepper in Iran. Plant Dis. 97:1259.

Hall, T. A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp. Ser. 41:95-98.

Hanley-Bowdoin, L., Settlage, S. B., Orozco, B. M., Nagar, S. and Robertson, D. 1999. Geminiviruses: models for plant DNA replication, transcription, and cell cycle regulation. Crit. Rev. Plant Sci. 18:71-106. crossref(new window)

Heydarnejad, J., Mozaffari, A., Massumi, H., Fazeli, R., Gray, A. J., Meredith, S., Lakay, F., Shepherd, D. N., Martin, D. P. and Varsani, A. 2009. Complete sequences of tomato leaf curl Palampur virus isolates infecting cucurbits in Iran. Arch. Virol. 154:1015-1018. crossref(new window)

Hussain, M., Mansoor, S., Iram, S., Fatima, A. N. and Zafar, Y. 2005. The nuclear shuttle protein of Tomato leaf curl New Delhi virus is a pathogenicity determinant. J. Virol. 79:4434-4439. crossref(new window)

Ito, T., Sharma, P., Kittipakorn, K. and Ikegami, M. 2008. Complete nucleotide sequence of a new isolate of tomato leaf curl New Delhi virus infecting cucumber, bottle gourd and muskmelon in Thailand. Arch. Virol. 153:611-613. crossref(new window)

Jeanmougin, F., Thompson, J. D., Gouy, M., Higgins, D. G. and Gibson, T. J. 1998. Multiple sequence alignment with Clustal X. Trends Biochem. Sci. 23:403-405. crossref(new window)

Kheyr-Pour, A., Bananej, K., Dafalla, G. A., Caciagli, P., Noris, E., Ahoonmanesh, A., Lecoq, H. and Gronenborn, B. 2000. Watermelon chlorotic stunt virus from the Sudan and Iran:sequence comparisons and identification of a whiteflytransmission determinant. Phytopathology 90:629-635. crossref(new window)

King, A. M. Q., Adams, M. J., Carstens, E. B. and Lefkowitz, E. J. 2012. Virus taxonomy: ninth report of the international committee on the taxonomy of viruses. Elsevier Academic Press, Amsterdam, The Netherlands.

Mansoor, S., Briddon, R. W., Zafar, Y. and Stanley, J. 2003. Geminivirus disease complexes: an emerging threat. Trends Plant Sci. 8:128-134. crossref(new window)

Mansoor, S., Zafar, Y. and Briddon, R. W. 2006. Geminivirus disease complexes: the threat is spreading. Trends Plant Sci. 11:209-212. crossref(new window)

Massumi, H., Samei, A., Hossein Pour, A., Shaabanian, M. and Rahimian, H. 2007. Occurrence, distribution, and relative incidence of seven viruses infecting greenhouse-grown cucurbits in Iran. Plant Dis. 91:159-163. crossref(new window)

Massumi, H., Shaabanian, M., Hosseini Pour, A., Heidarnejad, J. and Rahimian, H. 2009. Incidence of viruses infecting tomato and their natural hosts in the southeast and central regions of Iran. Plant Dis. 93:67-72. crossref(new window)

Mnari-Hattab, M., Zammouri, S., Belkadhi, M. S., Bellon Dona, D., ben Nahia, E. and Hajlaoui, M. R. 2015. First report of Tomato leaf curl New Delhi virus infecting cucurbits in Tunisia. New Dis. Rep. 31:21. crossref(new window)

Moriones, E. and Navas-Castillo, J. 2000. Tomato yellow leafcurl virus, an emerging virus complex causing epidemics worldwide. Virus Res. 71:123-134. crossref(new window)

Navot, N., Pichersky, E., Zeidan, M., Zamir, D. and Czosnek, H. 1991. Tomato yellow leaf curl virus-a whitefly-transmitted geminivirus with a single genomic component. Virology 185:151-161. crossref(new window)

Nei, M. and Kumar, S. 2000. Molecular evolution and phylogenetics. Oxford University Press, New York, NY, USA.

Pico, B., Diez, M. J. and Nuez, F. 1999. Improved diagnostic techniques for tomato yellow leaf curl virus in tomato breeding programs. Plant Dis. 83:1006-1012. crossref(new window)

Polston, J. E. and Anderson, P. K. 1997. The emergence of whitefly-transmitted geminiviruses in tomato in the Western hemisphere. Plant Dis. 81:1358-1369. crossref(new window)

Rishi, N. 2004. Current status of begomoviruses in the Indian subcontinent. Indian Phytopathol. 57:396-407.

Rojas, M. R., Jiang, H., Salati, R., Xoconostle-Cazares, B., Sudarshana, M. R., Lucas, W. J. and Gilbertson, R. L. 2001. Functional analysis of proteins involved in movement of the monopartite begomovirus, Tomato yellow leaf curl virus. Virology 291:110-125. crossref(new window)

Sambrook, J., Fritch, E. F. and Maniatis, T. 1989. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor Laboratory Press, New York, NY, USA.

Seal, S. E., van den Bosch, F. and Jeger, M. J. 2006. Factors influencing begomovirus evolution and their increasing global significance: implications for sustainable control. Crit. Rev. Plant Sci. 25:23-46. crossref(new window)

Shirazi, M., Mozafari, J., Rakhshandehroo, F. and Shams-Bakhsh, M. 2014. Genetic diversity, host range, and distribution of Tomato yellow leaf curl virus in Iran. Acta Virol. 58:128-136. crossref(new window)

Tamura, K. and Nei, M. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol. 10:512-526.

Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30:2725-2729. crossref(new window)

Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties, and weight matrix choice. Nucl. Acids Res. 22:4673-4680. crossref(new window)

Tiwari, A. K., Sharma, P. K., Khan, M. S., Snehi, S. K., Raj, S. K. and Rao, G. P. 2010. Molecular detection and identification of Tomato leaf curl New Delhi virus isolate causing yellow mosaic disease in Bitter gourd (Momordica charantia), a medicinally important plant in India. Medicinal Plants 2:117-123.

Tiwari, A. K., Snehi, S. K., Khan, M. S., Sharma, P. K., Raj, S. K. and Rao, G. P. 2012. Molecular detection and identification of Tomato leaf curl New Delhi virus associated with yellow mosaic and leaf curl disease of Luffa cylindrica in India. Indian Phytopathol. 65:80-84.

Varma, A. and Malathi, V. G. 2003. Emerging geminivirus problems: a serious threat to crop production. Ann. Appl. Biol. 142:145-164. crossref(new window)

Yazdani-Khameneh, S., Hamedi, A., Farahani, A. A., Hashemi, S., Golnaraghi, A. R. and Rakhshandehroo, F. 2013. Tomato mosaic virus on cucumber and potato in Iran. J. Plant Pathol. 95:665.

Zerbini, F. M., Andrade, E. C., Barros, D. R., Ferreira, S. S., Lima, A. T. M., Alfenas, P. F. and Mello, R. N. 2005. Traditional and novel strategies for geminivirus management in Brazil. Austral. Plant Pathol. 34:475-480. crossref(new window)