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Biological Control of Apple Anthracnose by Paenibacillus polymyxa APEC128, an Antagonistic Rhizobacterium
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  • Journal title : The Plant Pathology Journal
  • Volume 32, Issue 3,  2016, pp.251-259
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/PPJ.OA.01.2016.0015
 Title & Authors
Biological Control of Apple Anthracnose by Paenibacillus polymyxa APEC128, an Antagonistic Rhizobacterium
Kim, Young Soo; Balaraju, Kotnala; Jeon, Yongho;
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The present study investigated the suppression of the disease development of anthracnose caused by Colletotrichum gloeosporioides and C. acutatum in harvested apples using an antagonistic rhizobacterium Paenibacillus polymyxa APEC128 (APEC128). Out of 30 bacterial isolates from apple rhizosphere screened for antagonistic activity, the most effective strain was APEC128 as inferred from the size of the inhibition zone. This strain showed a greater growth in brain-heart infusion (BHI) broth compared to other growth media. There was a reduction in anthracnose symptoms caused by the two fungal pathogens in harvested apples after their treatment with APEC128 in comparison with non-treated control. This effect is explained by the increased production of protease and amylase by APEC128, which might have inhibited mycelial growth. In apples treated with different APEC128 suspensions, the disease caused by C. gloeosporioides and C. acutatum was greatly suppressed (by 83.6% and 79%, respectively) in treatments with the concentration of colony forming units (cfu)/ml compared to other lower dosages, suggesting that the suppression of anthracnose development on harvested apples is dose-dependent. These results indicated that APEC128 is one of the promising agents in the biocontrol of apple anthracnose, which might help to increase the shelf-life of apple fruit during the post-harvest period.
antagonistic activity;anthracnose;biological control;inoculum density;Paenibacillus polymyxa APEC128;
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Afanador-Kafuri, L., Minz, D., Maymon, M. and Freeman, S. 2003. Characterization of Colletotrichum isolates from tamarillo, passiflora, and mango in Colombia and identification of a unique species from the genus. Phytopathology 93:579-587. crossref(new window)

Agrawal, T. and Kotasthane, A. S. 2012. Chitinolytic assay of indigenous Trichoderma isolates collected from different geographical locations of Chhattisgarh in Central India. SpringerPlus 1:73. crossref(new window)

Alvarez, V. M., von der Weid, I., Seldin, L. and Santos, A. L. 2006. Influence of growth conditions on the production of extracellular proteolytic enzymes in Paenibacillus peoriae NRRL BD-62 and Paenibacillus polymyxa SCE2. Lett. Appl. Microbiol. 43:625-630. crossref(new window)

Ash, C., Priest, F. G. and Collins, M. D. 1993. Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. Antonie van Leeuwenhoek 64:253-260.

Bajpai, V. K., Choi, S. W., Cho, M. S. and Kang, S. C. 2009. Isolation and morphological identification of apple anthracnose fungus of Colletotrichum sp. KV-21. Korean J. Environ. Agri. 28:442-446. crossref(new window)

Cho, S. J., Lee, S. K., Cha, B. J., Kim, Y. H. and Shin, K. S. 2003. Detection and characterization of the Gloeosporium gloeosporioides growth inhibitory compound iturin A from Bacillus subtilis strain KS03. FEMS Microbiol. Lett. 223:47-51. crossref(new window)

Cook, R. J. 2000. Advances in plant health management in the twentieth century. Annu. Rev. Phytopathol. 38:95-116. crossref(new window)

Deacon, J. W. 1997. Modern mycology. Blackwell Scientific, Oxford, UK.

Dijksterhuis, J., Sanders, M., Gorris, L. G. and Smid, E. J. 1999. Antibiosis plays a role in the context of direct interaction during antagonism of Paenibacillus polymyxa towards Fusarium oxysporum. J. Appl. Microbiol. 86:13-21. crossref(new window)

Droby, S., Wisniewski, M., Macarisin, D. and Wilson, C. 2009. Twenty years of postharvest biocontrol research: is it time for a new paradigm? Postharvest Biol. Technol. 52:137-145. crossref(new window)

Ferreira, J. H. S., Matthee, F. N. and Thomas, A. C. 1991. Biological control of Eutypa lata on grapevine by an antagonistic strain of Bacillus subtilis. Phytopathology 81:283-287. crossref(new window)

Fleming, H. P., Etchells, J. L. and Costilow, R. N. 1975. Microbial inhibition by an isolate of Pediococcus from cucumber brines. Appl. Microbiol. 30:1040-1042.

Fossi, B. T., Tavea, F., Jiwoua, C. and Ndjouenkeu, R. 2009. Screening and phenotypic characterization of thermostable amylases producing yeasts and bacteria strains from some Cameroonian soils. Afr. J. Microbiol. Res. 3:504-514.

Gouzou, L., Burtin, G., Philippy, R., Bartoli, F. and Heulin, T. 1993. Effect of inoculation with Bacillus polymyxa on soil aggregation in the wheat rhizosphere: preliminary examination. Geoderma 56:479-491. crossref(new window)

Han, J. H., Shim, H., Shin, J. H. and Kim, K. S. 2015. Antagonistic activities of Bacillus spp. strains isolated from tidal flat sediment towards anthracnose pathogens Colletotrichum acutatum and C. gloeosporioides in South Korea. Plant Pathol. J. 31:165-175. crossref(new window)

Hankin, L. and Anagnostakis, S. L. 1977. Solid media containing carboxymethylcellulose to detect CX cellulose activity of micro-organisms. J. Gen. Microbiol. 98:109-115. crossref(new window)

Henz, G. P., Boiteux, L. S. and Lopes C. A. 1992. Outbreak of strawberry anthracnose caused by Colletotrichum acutatum in central Brazil. Plant Dis. 76:212.

Heulin, T., Berge, O., Mavingui, P., Gouzou, L. Hebbar, K. P. and Balandrean, J. 1994. Bacillus polymyxa and Rahnella aquatilis, the dominant $N_2$-fixing bacteria associated with rhizosphere in French soils. Eur. J. Soil Biol. 30:35-42.

Janisiewicz, W. J. and Korsten, L. 2002. Biological control of postharvest diseases of fruits. Annu. Rev. Phytopathol. 40:411-441. crossref(new window)

Kim, S. G., Khan, Z., Jeon, Y. H. and Kim, Y. H. 2009. Inhibitory effects of Paenibacillus polymyxa GBR-462 on Phytophthora capsici causing phytophthora blight in chili pepper. J. Phytopathol. 153:329-337.

Kloepper, J. W. 1992. Plant growth-promoting rhizobacteria as biological control agents. In: Soil microbial ecology: applications in agricultural and environmental management, ed. by F. B. Metting Jr., pp. 225-274. Marcel Dekker Inc., New York, NY, USA.

Lee, D. H., Kim, D. K., Jeon, Y. A., Uhm, J. Y., Hong, S. B. 2007. Molecular and cultural characterization of Colletotrichum spp. causing bitter rot of apples in Korea. Plant Pathol. J. 23:37-44. crossref(new window)

Lee, G. W., Ko, J. A., Oh, B. T., Choi, J. R., Lee, K. J., Chae, J. C. and Kamala-Kannan, S. 2012. Biological control of postharvest diseases of apples, peaches and nectarines by Bacillus subtilis S16 isolated from halophytes rhizosphere. Biocontrol Sci. Technol. 22:351-361. crossref(new window)

Lee, K. Y., Heo, K. R., Choi, K. H., Kong, H. G., Nam, J., Yi, Y. B., Park, S. H., Lee, S. W. and Moon, B. J. 2009. Characterization of a chitinase gene exhibiting antifungal activity from a biocontrol bacterium Bacillus licheniformis N1. Plant Pathol. J. 25:344-351. crossref(new window)

Li, J., Yang, Q., Zhao, L. H., Zhang, S. M., Wang, Y. X. and Zhao, X. Y. 2009. Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29. J. Zhejiang Univ. Sci. B 10:264-272. crossref(new window)

Liang, Y. L., Zhang, Z., Wu, M., Wu, Y. and Feng, J. X. 2014. Isolation, screening, and identification of cellulolytic bacteria from natural reserves in the subtropical region of China and optimization of cellulase production by Paenibacillus terrae ME27-1. Biomed. Res. Int. Online publication. doi:10.1155/2014/512497. crossref(new window)

Liu, J., Zhou, T., He, D., Li, X. Z., Wu, H., Liu, W. and Gao, X. 2011. Functions of lipopeptides bacillomycin D and fengycin in antagonism of Bacillus amyloliquefaciens C06 towards Monilinia fructicola. J. Mol. Microbiol. Biotechnol. 20:43-52. crossref(new window)

Mari, M., Neri, F. and Bertolini, P. 2009. Management of important diseases in Mediterranean high value crops. Stewart Postharvest Rev. 5:1-10.

Mavingui, P. and Heulin, T. 1994. In vitro chitinase and antifungal activity of a soil, rhizosphere and rhizoplane population of Bacillus polymyxa. Soil Biol. Biochem. 26:801-803. crossref(new window)

Nunes, C., Usall, J., Teixido, N., Torres, R. and Vinas, I. 2002. Control of Penicillium expansum and Botrytis cinerea on apples and pears with the combination of Candida sake and Pantoea agglomerans. J. Food Prot. 65:178-184. crossref(new window)

Onofre, S. B. and Antoniazzi, D. 2014. Behavior of the fungus Colletotrichum gloeosporioides (Penz & Sacc.), which causes bitter rot in apples after harvesting. Adv. Microbiol. 4:202-206. crossref(new window)

Park, J. W., Balaraju, K., Kim, J. W., Lee, S. W. and Park, K. S. 2013. Systemic resistance and growth promotion of chili pepper induced by an antibiotic producing Bacillus vallismortis strain BS07. Biol. Control 65:246-257. crossref(new window)

Roberts, W. K. and Selitrennikoff, C. P. 1988. Plant and bacterial chitinases differ in antifungal activity. J. Gen. Microbiol. 134:169-176.

Rosado, A. S. and Seldin, L. 1993. Production of a potentially novel anti-microbial substance by Bacillus polymyxa. World J. Microbiol. Biotechnol. 9:521-528. crossref(new window)

Sadhana, L. and Silvia, T. 2009. Ecology and biotechnological potential of Paenibacillus polymyxa: a minireview. Indian J. Microbiol. 49:2-10. crossref(new window)

Saravanakumar, D., Ciavorella, A., Spadaro, D., Garibaldi, A. and Gullino, M. L. 2008. Metschnikowia pulcherrima strain MACH1 outcompetes Botrytis cinerea, Alternaria alternata and Penicillium expansum in apples through iron depletion. Postharvest Biol. Technol. 49:121-128. crossref(new window)

SAS Institute. 1995. JMP statistics and graphics guide, version 3. SAS Institute, Cary, NC, USA. pp. 65-95.

Shaw, J., Lin, F. P., Chen, S. C. and Chen, H. C. 1995. Purification and properties of an extracellular ${\alpha}$-amylase from Thermus sp. Bot. Bull. Acad. Sin. 36:195-200.

Shishido, M., Massicotte, H. B. and Chanway, C. P. 1996. Effect of plant growth promoting Bacillus strains on pine and spruce seedling growth and mycorrhizal infection. Ann. Bot. 77:433-442. crossref(new window)

Singh, H. P. and Singh, T. A. 1993. The interaction of rockphosphate Bradyrhizobium, vesicular-arbuscular mycorrhizae and phosphate-solubilizing microbes on soybean grown in asub-Himalayan mollisol. Mycorrhiza 4:37-43. crossref(new window)

Tamura, K., Dudley, J., Nei, M. and Kumar, S. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24:1596-1599. crossref(new window)

Vichova, J., Stankova, B. and Pokorny, R. 2012. First report of Colletotrichum acutatum on tomato and apple fruits in the Czech Republic. Plant Dis. 96:769-770.

Weisburg, W. G., Barns, S. M., Pelletier, D. A. and Lane, D. J. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173:697-703. crossref(new window)

Xu, S. J., Hong, S. J., Choi, W. and Kim, B. S. 2014. Antifungal activity of Paenibacillus kribbensis strain T-9 isolated from soils against several plant pathogenic fungi. Plant Pathol J. 30:102-108. crossref(new window)

Yanez-Mendizabal, V., Usall, J., Vinas, I., Casals, C., Marin, S., Solsona, C. and Teixido, N. 2011. Potential of a new strain of Bacillus subtilis CPA-8 to control the major postharvest diseases of fruit. Biocontrol Sci. Technol. 21:409-426. crossref(new window)