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Deciphering the Role of Tyrosine Sulfation in Xanthomonas oryzae pv. oryzae Using Shotgun Proteomic Analysis
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  • Journal title : The Plant Pathology Journal
  • Volume 32, Issue 3,  2016, pp.266-272
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/PPJ.NT.12.2015.0273
 Title & Authors
Deciphering the Role of Tyrosine Sulfation in Xanthomonas oryzae pv. oryzae Using Shotgun Proteomic Analysis
Park, Hye-Jee; Park, Chang-Jin; Bae, Nahee; Han, Sang-Wook;
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 Abstract
A bacterial tyrosine sulfotransferase, RaxST, is required for activation of rice XA21-mediated immunity, and it catalyzes sulfation of tyrosine residues of Omp1X and RaxX in Xanthomonas oryzae pv. oryzae, a causal agent of bacterial blight in rice. Although RaxST is biochemically well-characterized, biological functions of tyrosine sulfation have not been fully elucidated. We compared protein expression patterns between the wildtype and a raxST knockout mutant using shotgun proteomic analysis. Forty nine proteins displayed a more than 1.5-fold difference in their expression between the wildtype and the mutant strains. Clusters of orthologous groups analysis revealed that proteins involved in cell motility were most abundant, and phenotypic observation also showed that the twitching motility of the mutant was dramatically changed. These results indicate that tyrosine sulfation by RaxST is essential for Xoo movement, and they provide new insights into the biological roles of RaxST in cellular processes.
 Keywords
proteomics;tyrosine sulfation;Xanthomonas oryzae pv. oryzae;
 Language
English
 Cited by
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