Advanced SearchSearch Tips
Deciphering the Role of Tyrosine Sulfation in Xanthomonas oryzae pv. oryzae Using Shotgun Proteomic Analysis
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : The Plant Pathology Journal
  • Volume 32, Issue 3,  2016, pp.266-272
  • Publisher : Korean Society of Plant Pathology
  • DOI : 10.5423/PPJ.NT.12.2015.0273
 Title & Authors
Deciphering the Role of Tyrosine Sulfation in Xanthomonas oryzae pv. oryzae Using Shotgun Proteomic Analysis
Park, Hye-Jee; Park, Chang-Jin; Bae, Nahee; Han, Sang-Wook;
  PDF(new window)
A bacterial tyrosine sulfotransferase, RaxST, is required for activation of rice XA21-mediated immunity, and it catalyzes sulfation of tyrosine residues of Omp1X and RaxX in Xanthomonas oryzae pv. oryzae, a causal agent of bacterial blight in rice. Although RaxST is biochemically well-characterized, biological functions of tyrosine sulfation have not been fully elucidated. We compared protein expression patterns between the wildtype and a raxST knockout mutant using shotgun proteomic analysis. Forty nine proteins displayed a more than 1.5-fold difference in their expression between the wildtype and the mutant strains. Clusters of orthologous groups analysis revealed that proteins involved in cell motility were most abundant, and phenotypic observation also showed that the twitching motility of the mutant was dramatically changed. These results indicate that tyrosine sulfation by RaxST is essential for Xoo movement, and they provide new insights into the biological roles of RaxST in cellular processes.
proteomics;tyrosine sulfation;Xanthomonas oryzae pv. oryzae;
 Cited by
Choi, H., Fermin, D. and Nesvizhskii, A. I. 2008. Significance analysis of spectral count data in label-free shotgun proteomics. Mol. Cell. Proteomics 7:2373-2385. crossref(new window)

da Silva, F. G., Shen, Y., Dardick, C., Burdman, S., Yadav, R. C., de Leon, A. L. and Ronald, P. C. 2004. Bacterial genes involved in type I secretion and sulfation are required to elicit the rice Xa21-mediated innate immune response. Mol. Plant-Microbe Interact. 17:593-601. crossref(new window)

Farzan, M., Mirzabekov, T., Kolchinsky, P., Wyatt, R., Cayabyab, M., Gerard, N. P., Gerard, C., Sodroski, J. and Choe, H. 1999. Tyrosine sulfation of the amino terminus of CCR5 facilitates HIV-1 entry. Cell 96:667-676. crossref(new window)

Gnanamanickam, S. S., Priyadarisini, V. B., Narayanan, N. N., Vasudevan, P. and Kavitha, S. 1999. An overview of bacterial blight disease of rice and strategies for its management. Curr. Sci. India 77:1435-1444.

Han, S. W., Lee, S. W., Bahar, O., Schwessinger, B., Robinson, M. R., Shaw, J. B., Madsen, J. A., Brodbelt, J. S. and Ronald, P. C. 2012. Tyrosine sulfation in a Gram-negative bacterium. Nat. Commun. 3:1153. crossref(new window)

Han, S. W., Park, C. J., Lee, S. W. and Ronald, P. C. 2008. An efficient method for visualization and growth of fluorescent Xanthomonas oryzae pv. oryzae in planta. BMC Microbiol. 8:164. crossref(new window)

Hopkins, C. M., White, F. F., Choi, S. H., Guo, A. and Leach, J. E. 1992. Identification of a family of avirulence genes from Xanthomonas oryzae pv. oryzae. Mol. Plant-Microbe Interact. 5:451-459. crossref(new window)

Kearns, D. B., Robinson, J. and Shimkets, L. J. 2001. Pseudomonas aeruginosa exhibits directed twitching motility up phosphatidylethanolamine gradients. J. Bacteriol. 183:763-767. crossref(new window)

Kehoe, J. W. and Bertozzi, C. R. 2000. Tyrosine sulfation: a modulator of extracellular protein-protein interactions. Chem. Biol. 7:R57-R61. crossref(new window)

Kovach, M. E., Elzer, P. H., Hill, D. S., Robertson, G. T., Farris, M. A., Roop, R. M. 2nd and Peterson, K. M. 1995. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 166:175-176. crossref(new window)

Matsubayashi, Y., Ogawa, M., Morita, A. and Sakagami, Y. 2002. An LRR receptor kinase involved in perception of a peptide plant hormone, phytosulfokine. Science 296:1470-1472. crossref(new window)

Mew, T. W. 1987. Current status and future prospects of research on bacterial blight of rice. Annu. Rev. Phytopathol. 25:359-382. crossref(new window)

Miller, R. M., Tomaras, A. P., Barker, A. P., Voelker, D. R., Chan, E. D., Vasil, A. I. and Vasil, M. L. 2008. Pseudomonas aeruginosa twitching motility-mediated chemotaxis towards phospholipids and fatty acids: specificity and metabolic requirements. J. Bacteriol. 190:4038-4049. crossref(new window)

Mitrophanov, A. Y. and Groisman, E. A. 2008. Signal integration in bacterial two-component regulatory systems. Genes Dev. 22:2601-2611. crossref(new window)

Monigatti, F., Hekking, B. and Steen, H. 2006. Protein sulfation analysis: a primer. Biochim. Biophys. Acta. 1764:1904-1913. crossref(new window)

Park, H. J., Jung, H. W. and Han, S. W. 2014a. Functional and proteomic analyses reveal that wxcB is involved in virulence, motility, detergent tolerance, and biofilm formation in Xanthomonas campestris pv. vesicatoria. Biochem. Biophys. Res. Commun. 452:389-394. crossref(new window)

Park, H. J., Lee, S. W. and Han, S. W. 2014b. Proteomic and functional analyses of a novel porin-like protein in Xanthomonas oryzae pv. oryzae. J. Microbiol. 52:1030-1035. crossref(new window)

Pruitt, R. N., Schwessinger, B., Joe, A., Thomas, N., Liu, F., Albert, M., Robinson, M. R., Chan, L. J., Luu, D. D., Chen, H., Bahar, O., Daudi, A., De Vleesschauwer, D., Caddell, D., Zhang, W., Zhao, X., Li, X., Heazlewood, J. L., Ruan, D., Majumder, D., Chern, M., Kalbacher, H., Midha, S., Patil, P. B., Sonti, R. V., Petzold, C. J., Liu, C. C., Brodbelt, J. S., Felix, G. and Ronald, P. C. 2015. The rice immune receptor XA21 recognizes a tyrosine-sulfated protein from a Gramnegative bacterium. Sci. Adv. 1:e1500245. crossref(new window)

Salzberg, S. L., Sommer, D. D., Schatz, M. C., Phillippy, A. M., Rabinowicz, P. D., Tsuge, S., Furutani, A., Ochiai, H., Delcher, A. L., Kelley, D., Madupu, R., Puiu, D., Radune, D., Shumway, M., Trapnell, C., Aparna, G., Jha, G., Pandey, A., Patil, P. B., Ishihara, H., Meyer, D. F., Szurek, B., Verdier, V., Koebnik, R., Dow, J. M., Ryan, R. P., Hirata, H., Tsuyumu, S., Won Lee, S., Seo, Y. S., Sriariyanum, M., Ronald, P. C., Sonti, R. V., Van Sluys, M. A., Leach, J. E., White, F. F. and Bogdanove, A. J. 2008. Genome sequence and rapid evolution of the rice pathogen Xanthomonas oryzae pv. oryzae PXO99A. BMC Genomics 9:204. crossref(new window)

Stone, M. J., Chuang, S., Hou, X., Shoham, M. and Zhu, J. Z. 2009. Tyrosine sulfation: an increasingly recognised posttranslational modification of secreted proteins. N. Biotechnol. 25:299-317. crossref(new window)

Tatusov, R. L., Galperin, M. Y., Natale, D. A. and Koonin, E. V. 2000. The COG database: a tool for genome-scale analysis of protein functions and evolution. Nucleic Acids Res. 28:33-36. crossref(new window)

Whitchurch, C. B., Leech, A. J., Young, M. D., Kennedy, D., Sargent, J. L., Bertrand, J. J., Semmler, A. B., Mellick, A. S., Martin, P. R., Alm, R. A., Hobbs, M., Beatson, S. A., Huang, B., Nguyen, L., Commolli, J. C., Engel, J. N., Darzins, A. and Mattick, J. S. 2004. Characterization of a complex chemosensory signal transduction system which controls twitching motility in Pseudomonas aeruginosa. Mol. Microbiol. 52:873-893. crossref(new window)