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Conjugation of mono-sulfobetaine to alkyne-PPX films via click reaction to reduce cell adhesion
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 Title & Authors
Conjugation of mono-sulfobetaine to alkyne-PPX films via click reaction to reduce cell adhesion
Chien, Hsiu-Wen; Keng, Ming-Chun; Chen, Hsien-Yeh; Huang, Sheng-Tung; Tsai, Wei-Bor;
 
 Abstract
A surface resisting protein adsorption and cell adhesion is highly desirable for many biomedical applications such as diagnostic devices, biosensors and blood-contacting devices. In this study, a surface conjugated with sulfobetaine molecules was fabricated via the click reaction for the anti-fouling purpose. An alkyne-containing substrate (Alkyne-PPX) was generated by chemical vapor deposition of 4-ethynyl-[2,2]paracyclophane. Azide-ended mono-sulfobetaine molecules were synthesized and then conjugated on Alkyne-PPX via the click reaction. The protein adsorption from 10% serum was reduced by 57%, while the attachment of L929 cells was reduced by 83% onto the sulfobetaine-PPX surface compared to the protein adsorption and cell adhesion on Alkyne-PPX. In conclusion, we demonstrate that conjugation of mono-sulfobetaine molecules via the click chemistry is an effective way for reduction of non-specific protein adsorption and cell attachment.
 Keywords
anti-fouling;sulfobetaine;cell adhesion;protein adsorption;click chemistry;
 Language
English
 Cited by
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