Advanced SearchSearch Tips
Molybdate Alters Sulfate Assimilation and Induces Oxidative Stress in White Clover (Trifolium repens L.)
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Molybdate Alters Sulfate Assimilation and Induces Oxidative Stress in White Clover (Trifolium repens L.)
Zhang, Qian; Lee, Bok-Rye; Park, Sang-Hyun; Jeong, Gi-Ok; Kim, Tae-Hwan;
  PDF(new window)
Molybdenum (Mo) in rhizosphere influences sulfate assimilation as well as a number of other physiological aspects. In this study, the activity of key enzymes in sulfate assimilatory pathways, such as ATP sulfurylase (ATPs), adenosine 5'-phosphosulphate reductase (APR), as well as the responses of reactive oxygen species (ROS), were analyzed to elucidate the metabolic and physiological effects of external Mo supply to detached leaves of Trifolium repens L. Mo supply with a range from 1 mM to 40 mM depressed the activity of ATPs throughout the entire time course. In the leaves exposed to 1 mM Mo, a continuous decrease in the activity of ATPs was confirmed by Native-PAGE. The APR activity was also declined by Mo treatment. The accumulation of and were not significant up to 10 mM Mo, whereas a remarked accumulation was detected under 40 mM Mo supply. The data suggest that an external supply of Mo has an inhibitory effect on sulfate assimilation, and induces oxidative stress only at an extremely high concentration.
Molybdate;Oxidative species;Sulfate assimilation;Trifolium repens;
 Cited by
Atkinson, N.J. and Urwin, P.E. 2012. The interaction of plant biotic and abiotic stresses: from genes to the field. Journal of Experimental Botany. 63:3523-3543. crossref(new window)

Barrie, F. and Taylor, B.F. 1994. Adenylyl sulfate reductases from thiobacilli. Method Enzymology. 243:393-400. crossref(new window)

Bradford, M.M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 72:248-254. crossref(new window)

Chalker-Scott, L. 1999. Environmental significance of anthocyanins in plant stress responses. Photochemistry and Photobiology. 70:1-9. crossref(new window)

De Kok, L.J., Castro, A., Durenkamp, M., Kralewska, A., Posthumus, F.S., Elisabeth, C., Stuiver, E., Yang, L. and Stulen, I. 2005. Pathways of plant sulphur uptake and metabolism-an overview. Landbauforschung Volkenrode. 283:5-13.

Hale, K.L., McGrath, S.P., Lombi, E., Stack, S.M., Terry, N., Pickering, I.J., George, G.N. and Pilon-Smits, E.A.H. 2001. Molybdenum sequestration in Brassica species. A role for anthocyanins? Plant Physiology. 126:1391-1402. crossref(new window)

Hell, R. and Mendel, R.R. 2010. Cell Biology of Metals and Nutrients, Plant Cell Monographs 17, Springer Press, Berlin Heidelberg.

Lin, C.Z. and Kao, C.H. 2001. Cell wall peroxidase activity, hydrogen peroxide level and NaCl-inhibited root growth of rice seedlings. Plant and Soil. 230:135-143. crossref(new window)

Mendel, R.R. 2013. The molybdenum cofactor. Journal of Biological Chemistry. 288:13165-13172. crossref(new window)

Osslund, T., Chandler, C. and Seqel, I.H. 1982. ATP sulfurylase from higher plants. Plant Physiology. 70:39-45. crossref(new window)

Reuveny, Z. 1977. Depression of ATP sulfurylase by the sulfate analogs molybdate and selenite in cultured tobacco cells. Proceedings of the National Academy of Sciences of the United States of America. 74:619-622. crossref(new window)

Rout, G.R. and Das, P. 2002. Rapid hydroponic screening for molybdenum tolerance in rice through morphological and biochemical analysis. Rostlinna Vyroba. 48:505-512.

Scheerer, U., Haensch, R., Mendel, R.R., Kopriva, S., Rennenberg, H. and Herschbach, C. 2010. Sulphur flux through the sulphate assimilation pathway is differently controlled by adenosine 5'-phosphosulphate reductase under stress and in transgenic poplar plants overexpressing ${\gamma}$-ECS, SO, or APR. Journal of Experimental Botany. 61:609-622. crossref(new window)

Schiavon, M., Pittarello, M., Pilon-Smits, E.A.H., Wirtz, M., Hell, R. and Malagoli, M. 2012. Selenate and molybdate alter sulfate transport and assimilation in Brassica juncea L. Czern: implications for phytoremediation. Environmental and Experimental Botany. 75:41-51. crossref(new window)

Shinmachi, F., Buchner, P., Stroud, J.L., Parmar, S., Zhao, F.J., McGrath, S.P. and Hawkesford, M.J. 2010. Influence of sulfur deficiency and the distribution of sulfur, selenium, and molybdenum in wheat. Plant Physiology. 153:327-336. crossref(new window)

Simonovic, A., Gaddameedhi, S. and Anderson, M.D. 2004. In-gel precipitation of enzymatically released phosphate. Analytical Biochemistry. 334:312-317. crossref(new window)

Tewari, R.K., Kumar, P. and Sharma, P.N. 2010. Morphology and oxidative physiology of sulphur-deficient mulberry plants. Environmental and Experimental Botany. 68:301-308. crossref(new window)

Upadhyaya, H.D., Dwivedi, S.L., Gowda, C.L.L. and Singh, S. 2007. Identification of diverse germplasm lines for agronomic traits in a chickpea (Cicerarietinum L.) core collection for use in crop improvement. Field Crops Research. 100:320-326. crossref(new window)

Uttara, B., Singh, A.V., Zamboni, P. and Mahajan, R.T. 2009. Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Current Neuropharmacology. 7:65-74. crossref(new window)

Wang, A.G. and Luo, G.H. 1990. Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants. Plant Physiology Communications. 6:55-57.

Zhang, Y. and Gladyshev, V.N. 2008. Molybdoproteomes and evolution of molybdenum utilization. Journal of Molecular Biology. 379:881-899. crossref(new window)