Advanced SearchSearch Tips
Addition of Novel Lactobacillus plantarum KCC-10 and KCC-19 to Improve Fermentation Quality and Characterization of Italian Ryegrass Silage
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Addition of Novel Lactobacillus plantarum KCC-10 and KCC-19 to Improve Fermentation Quality and Characterization of Italian Ryegrass Silage
Srigopalram, Srisesharam; Ilavenil, Soundharrajan; Vijayakumar, Mayakrishnan; Park, Hyung Soo; Lee, Kyung Dong; Choi, Ki Choon;
  PDF(new window)
An investigation was carried out to evaluate the potential role of Lactobacillus Plantarum KCC-10 and KCC-19 on the quality and fermentation characterization of Italian ryegrass (IRG) silages. The physiochemical properties of IRG silage such as crude protein content, neutral detergent fiber, acid detergent fiber, total digestible nutrient and in vitro dry matter digestibility were not affected by KCC-10 and KCC-19. The pH of IRG silage in KCC-10 and KCC-19 treatments decreased compared to the control (p<0.05), while the lactic acid content in KCC-10 and KCC-19 treatments increased compared to the control (p<0.05). In addition, the number of lactic acid bacteria (LAB) in the KCC-10 treatment increased compared to the control (p<0.05). The number of lactic acid bacteria in KCC-19 increased, but there was no significant difference in all treatments. Therefore, we recommend L. plantarum KCC-10 and KCC-19 as potential additive candidates in IRG silage with lots of advantages.
Italian ryegrass;Lactobacillus plantarum;Silage;Lactic acid;Quality;
 Cited by
Application of customised bacterial inoculants for grass haylage production and its effectiveness on nutrient composition and fermentation quality of haylage, 3 Biotech, 2017, 7, 5  crossref(new windwow)
Ashbell, G., Weinberg, Z.G., Hen, Y. and Filya, I. 2002. The effects of temperature on the aerobic stability of wheat and corn silages. Journal of Industrial Microbiology and Biotechnology. 28:261-263. crossref(new window)

AOAC. 1990. Official method of analysis. 15thed. Washington, DC.

Avila, C.L.S., Pinto, J.C., Oliveira, D.P. and Schwan, R.F. 2012. Aerobic stability of sugar cane silages with a novel strain of Lactobacillus sp. isolated from sugar cane. Brazilian Journal Animal Science. 41:249-255.

Avila, C.L.S., Valeriano, A.R., Pinto J.C., Fiqueiredo, H.C.P., Rezende, A.V. and Schwan, R.F. 2010. Chemical and microbiological characteristics of sugarcane silages treated with microbial inoculants. Revista Brasileira de Zootecnia. 39:25-32. crossref(new window)

Cai, Y., Benno, M. and Kumai, S. 1999. Effect of applying lactic acid bacteria isolated from forage crops on fermentation characteristics and aerobic deterioration of silage. Journal of Dairy Science. 82:520-526. crossref(new window)

Cao, Y., Takahashi, T., Horiguchi, K., Yoshida, N. and Cai, Y. 2010. Methane emissions from sheep fed fermented or non-fermented total mixed ration containing whole-crop rice and rice bran. Animal Feed Science and Technology. 157:72-78. crossref(new window)

Cooke, K.M., Bernard, J.K. and West, J.W. 2009. Performance of lactating dairy cows fed ryegrass silage and corn silage with ground corn, steam flaked corn, or hominy feed. Journal of Dairy Science. 92:1117-1123. crossref(new window)

Danner, H., Holzer, M., Mayrhuber, E. and Braun, R. 2003. Acetic acid increases stability of silage under aerobic conditions. Applied and Environmental Microbiology. 69:562-567. crossref(new window)

Ely, L.O., Sudweeks, E.M., and Moon, N.J., 1981. Inoculation with Lactobacillus plantarum of alfalfa, corn, sorghum, and wheat silages. Journal of Dairy Science. 64:2378-2387. crossref(new window)

Filya, I. 2003a. The effect of Lactobacillus buchneri and L. plantarum on the fermentation, aerobic stability, and ruminal degradability of low dry matter corn and sorghum silages. Journal of Dairy Science. 86:3575-3581. crossref(new window)

Filya, I. 2003b. The effect of Lactobacillus buchneri, with or without homofermentative lactic acid bacteria, on the fermentation, aerobic stability, and ruminal degradability of wheat, sorghum, and maize silages. Journal of Applied Microbiology. 95:1080-1086. crossref(new window)

Filya, I., Karabulut, A. and Sucu, E. 2002. The effect of Lactobacillus plantarum and Lactobacillus buchneri on the fermentation, aerobic stability and ruminal degradability of corn silage in warm climate. In Proceedings of the 13th International Silage Conference.

Filya, I.R.E., Muck, F. and Contreras-Govea, E. 2007. Inoculant effects on alfalfa silage: Fermentation products and nutritive value. Journal of Dairy Science. 90:5108-5114. crossref(new window)

Giraffa, G., Chanishvili, N. and Widyastuti, Y. 2010. Importance of lactobacilli in food and feed biotechnology. Research in Microbiology. 161:480-487. crossref(new window)

Holland, C., Kezar W., Kautz, W.P., Lazowski, E.J., Mahanna W.C. and Reinhart, R. 1990. Pioneer forage manual: A nutritional guide. Pioneer Hi-Bred International, Inc. Des moines, IA. pp. 1-55.

Ilavenil, S., Arasu, M.V., Vijayakumar, M., Jung, M.W., Park, H.S., Lim, Y.C. and Choi, K.C. 2014. Lactobacillus plantarum Improves the Nutritional Quality of Italian ryegrass with Alfalfa Mediated Silage. Journal of the Korean Society of Grassland and Forage Science. 34(3):174-178. crossref(new window)

Imai, A. 2000. Silage making and utilization of high moisture by-products. Japanese Society of Grassland Science. 47:307-310.

Jatkauskas, J. and Vrotniakiene, V. 2004. Fermentation characteristics and nutritive value of inoculated corn silage. Luzern, Switzerland. In Proceedings of the 20th general meeting of EGF, pp. 1077-1079.

Kristensen, N.B., Storm, A., Raun, B.M.L., Rojen, B.A. and Harmon, D.L. 2007. Metabolism of silage alcohols in lactating dairy cows. Journal of Dairy Science. 90:1364-1377. crossref(new window)

Merry, R.J., Jones, R. and Theodorou, M.K., 2000. The conservation of grass in Grass. Its Production and Utilisation. A. Hopkins, Ed., Blackwell Science, Oxford, UK, 3rd edition.

Miller, T.L. and Wolin, M.J. 1974. A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Applied Microbiology. 27:985-987.

Moon, N.J. 1983. Inhibition of the growth of acid tolerant yeasts by acetate, lactate and propionate and their synergistic mixtures. Journal of Applied Bacteriology. 55(3):453-460. crossref(new window)

Moore, J.E. 1970. Procedure for the two-stage in vitro digestion of forage. University of Florida, Department of Animal Science.

Muck, E., Contreras, F.E. and Mertens, D.R. 2007. Silage inoculants effects on in vitro rumen fermentation. Journal of Animal Science. 85:284.

Nishino, N., Yoshida, M., Shiota, H. and Sakaguchi, E. 2003. Accumulation of 1,2-propanediol and enhancement of aerobic stability in whole crop maize silage inoculated with Lactobacillus buchneri. Journal of Applied Microbiology. 94:800-807. crossref(new window)

O'Brien, M., O'Kiely, P., Forristal, P.D. and Fuller, H.T. 2007. Quantification and identification of fungal propagules in well-managed baled grass silage and in normal on-farm produced bales. Animal Feed Science and Technology. 132:283-297. crossref(new window)

Olmos Colmenero, J.J. and Broderick, G.A. 2006. Effect of Dietary Crude Protein Concentration on Milk Production and Nitrogen Utilization in Lactating Dairy Cows. Journal of Dairy Science. 89:1704-1712. crossref(new window)

Saarisalo, E., Skytta, E., Haikara, A., Jalava, T. and Jaakkola, S. 2007. Screening and selection of lactic acid bacteria strains suitable for ensiling grass. Journal of Applied Microbiology. 102(2):327-336.

Seo, S., Kim, W.H., Kim, J.G., Choi, G.J., Kim, K.Y., Cho, W.M., Park, B.Y. and Kim, Y.H. 2010. Effect of Whole Crop Barley Silage Feeding on the Growth Performance, Feed Requirement and Meat Quality of Hanwoo Steers. Journal of the Korean Society of Grassland and Forage Science. 30(3):257-266. crossref(new window)

Stokes, M.R. 1992. Effects of an enzyme mixture, an inoculant, and their interaction on silage fermentation and dairy production. Journal of Dairy Science. 75:764-773. crossref(new window)

Tyrolova, Y. and Vyborna, A. 2008. Effect of the stage of maturity on the leaf percentage of lucerne and the effect of additives on silage characteristics. Czech Journal of Animal Science. 53(8):330-335.

Valan Arasu, M., Jung, M.W., Ilavenil, S., Jane, M., Kim, D.H., Lee, K.D., Park, H.S., Huh, T.Y., Choi, G.J., Lim, Y.C., Al-Dhabi N.A. and Choi, K.C. 2013. Isolation and characterization of antifungal compound from Lactobacillus plantarum KCC-10 from forage silage with potential beneficial properties. Journal of Applied Microbiology. 115(5):1172-85. crossref(new window)

Valan Arasu, M., Jung, M.W., Kim, D.H., Ilavenil, S., Lee, K.D., Choi, G.J., Al-Dhabi, NA and Choi, K.C. 2014. Isolation and characterization of Lactobacillus plantarum KCC-19 from crimson silage. Journal of Pure and Applied Microbiology. 8(5):3575- 3587.

Van Soest, P.J., In H.G., Jung, D.R., Buxton, R.D. and Ralph eds, J. 1993. Cell wall matrix interactions and degradation session synopsis Forage Cell Wall Structure and Digestibility. American Society of Agronomy, Madison. 377-395.

Weinberg, Z.G. and Muck, R.E. 1996. New trends in development and use of inoculants for silage. FEMS Microbiology Reviews. 19:53-68.

Weinberg, Z.G., Ashbell, G., Hen, Y., Azrieli, A., Szakacs, G. and Filya, I. 2002. Ensiling whole-crop wheat and corn in large containers with Lactobacillus plantarum and Lactobacillus buchneri. Journal of Industrial Microbiology and Biotechnology. 28:7-11. crossref(new window)

Wilkins, R.J., Syrjala-Qvist, L. and Bolsen, K.K. 1999. The future role of silage in sustainable animal production," in Proceedings of the 12th International Silage Conference, pp. 23-40, Uppsala, Sweden.