Publisher : The Korean Society of Grassland and Forage Science
DOI : 10.5333/KGFS.2015.35.4.316
Title & Authors
Effect of Physical Pre-treatment of Mature Seed in Callus Formation and Plant Regeneration of Zoysiagrass Ahn, Na-Young; Kim, Yong-Goo; Rahman, Md. Atikur; Bae, Eun Ji; Choi, Su Min; Lee, Kwang Soo; Lee, Byung-Hyun;
In order to improve the transformation efficiency of zoysiagrass by increasing the frequency of callus formation from mature seeds and plant regeneration, the effect of pre-treatment with sea sand was examined. Mature zoysiagrass seeds were given 10 min of swelling time before sea sand treatment using a sea sand and seed ratio of 1 : 1 and a vortex shaking speed of 6 (1,000 rpm) for 10 min. The seeds showed increased callus formation that was more than 2 times the rate in the control. In addition, plant regeneration efficiency was also increased when embryogenic callus induced from sea sand-treated mature seeds was cultured in regeneration medium. These results will be very helpful for improving the genetic transformation frequency of zoysiagrass, a recalcitrant monocot grass.
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