Advanced SearchSearch Tips
Effect of Microbial Flora and Inoculation of Probiotics on Fermenting Characteristics of Naked Barley Grain (Hordeum Vulgare L.)
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effect of Microbial Flora and Inoculation of Probiotics on Fermenting Characteristics of Naked Barley Grain (Hordeum Vulgare L.)
Ahn, Hye-Jin; Kim, Ki Hyun; Jo, Eun Seok; Kim, Jo Eun; Kim, Kwang-Sik; Kim, Young Hwa; Song, Tae Hwa; Park, Jong Ho; Kang, Hwan Ku; Jang, Sun Sik; Oh, Young Kyoon; Cheon, Dong Won; Seol, Kuk-Hwan;
  PDF(new window)
This study was performed to analyze the resident microbial flora and the effects of probiotic inoculation on the fermentation characteristics of whole grain naked barley (Hordeum Vulgare L.) with the goal of evaluating the possibility of utilization as fermented feedstuff. Naked barley grains were harvested 35 days after heading, and the microbial flora was analyzed using MALDI-TOF mass spectrometer. After inoculation of commercial microbes to the naked barley grain (BT), the pH and number of bacteria, such as aerobic bacteria, lactic acid bacteria, yeast and E. coli, were measured and compared with the non-inoculated control (BC). A total of 122 colonies was isolated from the naked barley grain and the most popular bacteria species was Staphylococcus xylosus (n = 30, 24.59%). The pH value decreased more rapidly in BT than in BC, and was significantly lower after 7 days of fermentation at and , respectively. The number of aerobic bacteria, lactic acid bacteria and yeast showed an increasing trend within the first 7 days of fermentation, however, their numbers decreased at 28 and 42 days of fermentation. The population of lactic acid bacteria in BT was higher than in BC, but there was no significant different at 7 days of fermentation, with respective levels of and (p>0.05). The initial number of E. coli was very high in the naked barley grain but subsequently decreased significantly. After 7 days of fermentation, E. coli was not detected in either BT or BC samples. From these results, it appears that the fermentation of naked barley grain proceeded adequately after 7 days, and that fermentation contributes to the safety of naked barley grain during storage.
Naked barley grain;Hordeum Vulgare L.;Microbial flora;Fermentation;
 Cited by
Fermentative characteristics of wheat bran direct-fed microbes inoculated with starter culture,;;;;;;;;

농업과학연구, 2016. vol.43. 3, pp.387-393 crossref(new window)
Anugwa, F.O.I., Varel, V.H., Dickson, J.S., Pond, W.G. and Krook, L.P. 1989. Effects of dietary fiber and protein concentration on growth, feed efficiency, visceral organ weights and large intestine microbial populations of swine. Journal of Nutrition. 119:879-886. crossref(new window)

Cho, M.Z. 1995. Studies on the variation of diet fiber content according to pearling ratio of barley. Journal of Korean Home Economics Association. 33:181-186.

Cho, S.B., Kim, D.W., Yang, S.H., Park, K.H., Choi, D.Y., Yoo, Y.H. and Hwang, O.H. 2012. Establishment of producing condition of fermentation feed for swine. Journal of Animal Environmental Science. 18:137-144.

Chung, Y.K., Chea, B.J., Kim, J.H., Chu, K.S. and Han, I.K. 1998. An evaluation of barely in finisher pig diet for high quality pork production. Korean Journal of Animal Nutrition and Feedstuffs. 22:15-20.

Ghanem, N.B., Yusef, H.H. and Mahrouse, H.K. 2000. Production of Aspergillus terreus xylanase in solid-state cultures: application of the Plackett-Burman experimental design to evaluate nutritional requirements. Bioresource Technology. 73:113-121. crossref(new window)

Ju, J.I., Lee, K.S., Min, H.I., Lee, B.J., Kwon, B.G., Gu, J.H. and Oh, M.J. 2007. Changes in physicochemical characteristics of green barley according to days after heading. Korean Journal of Crop Science. 52:36-44.

Jung, E.Y., Yum, C.A., Kim, S.K. and Jang, M.S. 1987. The chemical composition of pearled cutted, and pressed barleys. Korean Journal of Food Science and Technology. 19:290-294.

Kim, H.Y., Chu, G.M., Kim, S.C., Ha, J.H., Kim, J.H., Lee, S.D. and Song, Y.M. 2009. The nutritive value of grains from barley cultivars (Wooho, Youngyang, Yuyeon). Journal of Agriculture and Life Science. 46:69-78.

Kim, J.G., Ham, J.S., Chung, E.S., Park, H.S., Lee, J.K., Jung, M.W., Choi, K.C., Jo, N.C. and Seo, S. 2009. Evaluation of fermentation ability of microbes for whole crop barley silage inoculant. Journal of the Korean Society of Grassland and Forage Science. 29:235-244. crossref(new window)

Lee, Y.T. and Jung, J.Y. 2003. Quality characteristics of barley ${\beta}$-glucan enriched noodles. Korean Journal of Food Science and Technology. 35:405-409.

Lee, S.W., Ham, S.N., Shin, T.S., Kim, H.K., Yeon, I.J. and Kim, K.Y. 2009. Resource of food waste using indigenous bacteria isolated from soils. Journal of Korean Society of Environment Engineering. 31:35-41.

McDonald, P. and Edwards, P.A. 1976. The influence of conservation methods on digestion and utilization of forages by ruminants. Proceedings of the Nutrition Society. 35:201-211.

Seale, D.R. 1986. Bacterial inoculants as silage additive. Journal of Applied Bacteriology. 61:9s-26s.

Shin, S.O., Yoo, J.S., Lee, J.S., Jang, H.D., Kim, H.J., Chen, Y.J., Cho, J.H. and Kim, I.H. 2008. Effects of high protein diet containing barley on growth performance and carcass characteristics in finishing pigs. Korean Journal for Food Science of Animal Resources. 28:349-354. crossref(new window)

Song, Y.M. and Ha, J.H. 2007. A study on the evaluation of feed value for manufacturing fermentation using feedstuffs. Journal of Agricultural Technology Research Institute (Jinju National University) 20:153-162.

Stockes, M.R. 1992. Effects of an enzyme mixture, an inoculant, and their interaction on silage fermentation and dairy production. Journal of Dairy Science. 75:764-773. crossref(new window)

Suh, J.S., Noh, H.J., Kwon, J.S., Weon, H.Y. and Hong, S.Y. 2010. Distribution map of microbial diversity in agricultural land. Korean Journal of Soil Science and Fertilizer. 43:995-1001.

Yun, S.K., Park, T.I., Seo, J.H., Kim, K.H., Song, T.H., Park, K.H. and Han, O.K. 2009. Effect of harvest time and cultivars on forage yield and quality of whole crop barley. Journal of the Korean Society of Grassland and Forage Science. 29:121-128. crossref(new window)

Cai, Y. 2005. Quality improving technique of whole crop silage. International Symposium Proceedings of Livestock Research Institute on Production and Utilization of Whole Crop Rice . pp. 103-136.

Han, M.S. 1987. Study of utilization of local feed resources : effect of domestic barley on carcass characteristics of pig. Thesis for Master Degree, Konkuk University.