Advanced SearchSearch Tips
Anti-Inflammatory Effects of Chrysanthemum indicum Water Extract in RAW 264.7 Cell as a Whole Plant
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Anti-Inflammatory Effects of Chrysanthemum indicum Water Extract in RAW 264.7 Cell as a Whole Plant
Kang, Kyoungah;
  PDF(new window)
Purpose: Chrysanthemum indicum (CHI) has been used for edible and medical purposes for a long time in Korea. The purpose of this study was to evaluate the anti-inflammatory effects of CHI water extract in lipopolysaccharides (LPS)-induced RAW 264.7 macrophage cells. Methods: To investigate the anti-inflammatory effects on LPS-induced RAW 264.7 macrophage cells, CHI extract as a whole plant was used in this study. RAW 264.7 cells were treated with various concentrations of CHI extract (1, 10, and ). After that Nitric Oxide (NO), inducible nitric oxide synthase (iNOS), interleukin (IL)-, cyclooxygenase (COX)-2 and prostaglandin () expression level were measured. Results: CHI extract significantly suppressed the LPS-induced NO production and decreased the level of iNOS, IL-, COX-2 messenger ribonucleic acid (mRNA) expression and also the down regulation of expression in a dose-dependent manner. Conclusion: The present study suggested that CHI extract can be substituted for anti-inflammatory drugs and provide a safe and effective non pharmacological therapeutic approach.
Anti-inflammation;Chrysanthemum indicum;RAW 264.7 cell;
 Cited by
Braun CA, Anderson CM. Pathophysiology functional alterations in human health. Philadelphia: Lippincott; 2007. p. 38-52.

Chen JN, de Mejia EG, Wu JSB. Inhibitory effect of a glycoprotein isolated from golden oyster mushroom (pleurotus citrinopileatus) on the lipopolysaccharide-induced inflammatory reaction in RAW 264.7 macrophage. Journal of Agricultural and Food Chemistry. 2011;59(13):7092-7097. crossref(new window)

Olefsky JM, Glass CK. Macrophages, inflammation, and insulin resistance. Annual Review of Physiology. 2010;72:219-246. crossref(new window)

Su GL. Lipopolysaccharides in liver injury: Molecular mechanisms of kupffer cell activation. American Journal of Physiology. 2002;283(2):256-265.

Kubes P. Inducible nitric oxide synthase: A little bit of good in all of us. Gut. 2000;47:6-9. crossref(new window)

Tracey KJ. The inflammatory reflex. Nature. 2002;420(6917):853-859. crossref(new window)

Nathan. C. Points of control in inflammation. Nature. 2002;420(6917):846-852. crossref(new window)

Byun MW. Anti-inflammatory activity of austroinulin from stevia rebaudiana in LPS-induced RAW 264.7 cells. Journal of the Korean Society of Food Science and Nutrition. 2012;41(4):456-461. crossref(new window)

Park SM, Byun SH, Kim YW, Cho IJ, Kim SC. Inhibitory effect of Mori Folium ethanol extract on pro-inflammatory mediator in lipopolysaccharide - activated RAW 264.7 cells. The Korea Journal of Herbology. 2012; 27(3):31-38.

Oates JC, Ruiz P, Alexander A, Pippen A MM, Gilkeson GS. Effect of late modulation of nitric oxide production on murine lupus. Clinical Immunology and Immunopathology. 1997;83(1):86-92. crossref(new window)

Zingarelli B, Szabo C, Salzman AL. Reduced oxidative and nitrosative damage in murine experimental colitis in the absence of inducible nitric oxide synthase. Gut. 1999;45(2):199-209. crossref(new window)

Boileau C, Martel-Pelletier J, Brunet J, Tardif G, Schrier D, Flory C, et al. Oral treatment with PD-0200347, an alpha-2delta ligand, reduces the development of experimental osteoarthritis by inhibiting metalloproteinases and inducible nitric oxide synthase gene expression and synthesis in cartilage chondrocytes. Arthritis and Rheumatology. 2005;52(2):488-500. crossref(new window)

Kim JY, Jung KS, Jeong HG. Suppressive effects of the kahweol and cafestol on cyclooxygenase-2 expression in macrophages. FEBS Letters. 2004;569(1-3):321-326. crossref(new window)

Crofford LJ. Use of NSAIDs in treating patients with arthritis. Arthritis Research and Therapy. 2013;15(Suppl 3):S2. crossref(new window)

Kim MY, Son IP, Kim SY, Song YS, Chang HK, Yoo SJ, et al. Anti-inflammatory effect of green tea cell water in activated RAW 264.7 cells with lipopolysaccharide. Journal of Asthma, Allergy and Clinical Immunology. 2012;32(2):115-121.

Lee E. Anti-inflammatory activity of chrysanthemum indicum L. extract in lipopolysaccharide-treated rats. Natural Product Sciences. 2009;15(2):55-59.

Woo JH, Shin SL, Lee CH. Antioxidant effects of ethanol extracts from flower species of compositae plant. Journal of the Korean Society of Food Science and Nutrition. 2010;39(2):159-164. crossref(new window)

Luyen BT, Tai BH, Thao NP, Cha JY, Lee HY, Lee YM, et al. Anti-inflammatory components of chrysanthemum indicum flowers. Bioorganic and Medicinal Chemistry Letters. 2015;25(2):266-269. 11.054 crossref(new window)

Cheon MS, Yoon TS, Lee DY, Choi GY, Moon BC, Lee AY, et al. Chrysanthemum indicum Linne extract inhibits the inflammatory response by suppressing NF-${\kappa}$B and MAPKs activation in lipopolysaccharide-induced RAW 264.7 macrophages. Journal of Ethnopharmacology. 2009;122(3):473-477. crossref(new window)

Kang MJ, Shin SR, Kim KS. Antioxidative and free radical scavenging activity of water extract from dandelion (Taraxacum officinale). Korean Journal of Food Preservation. 2002;9(2):253-259.

Cheng W, Li J, You T, Hu C. Anti-inflammatory and immunomodulatory activities of the extracts from the inflorescence of chrysanthemum indicum linne. Journal of Ethnopharmacology. 2005;101(1-3):334-337. crossref(new window)

Kumar V. Innate lymphoid cells: New paradigm in immunology of inflammation. Immunology Letters. 2014;157(1-2):23-37. crossref(new window)

Lee MY, Lee JA, Seo CS, Ha H, Lee H, Son JK, et al. Anti-inflammatory activity of angelica dahurica ethanolic extract on RAW 264.7 cells via upregulation of heme oxygenase-1. Food and Chemical Toxicology. 2011;49(5):1047-1055. crossref(new window)

Ahmed S, Rahman A, Hasnain A, Lalonde M, Goldberg VM, Haqqi TM. Green tea polyphenol epigallocatechin-3-gallate inhibits the IL-1b-induced activity and expression of cyclooxygenase-2 and nitric oxide synthase-2 in human chondrocytes. Free Radical Biology and Medicine. 2002;33(8):1097-1105. crossref(new window)