Advanced SearchSearch Tips
Homology Modeling of GPR18 Receptor, an Orphan G-protein-coupled Receptor
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of the Chosun Natural Science
  • Volume 6, Issue 1,  2013, pp.16-20
  • Publisher : The Research Institute of Chosun Natural Science
  • DOI : 10.13160/ricns.2013.6.1.016
 Title & Authors
Homology Modeling of GPR18 Receptor, an Orphan G-protein-coupled Receptor
Kothandan, Gugan; Cho, Seung Joo;
  PDF(new window)
G-protein-coupled receptor (GPCR) superfamily is the largest known receptor family, characterized by seven transmembrane domains and considered to be an important drug target. In this study we focused on an orphan GPCR termed as GPR18. As there is no X-ray crystal structure has been reported for this receptor, we report on a homology model of GPR18. Template structure with high homology was used for modeling and ten models were developed. A model was selected and refined by energy minimization. The selected model was further validated using various parameters. Our results could be a starting point for further structure based drug design.
Structure-Based Drug Design;Homology Modeling;GPR18;GPCR;
 Cited by
Theoretical analysis of somatostatin receptor 5 with antagonists and agonists for the treatment of neuroendocrine tumors, Molecular Diversity, 2017, 21, 2, 367  crossref(new windwow)
Isolation, purification and characterization of proteinaceous fungal α-amylase inhibitor from rhizome of Cheilocostus speciosus (J.Koenig) C.D.Specht, International Journal of Biological Macromolecules, 2018, 111, 39  crossref(new windwow)
Structural characterization of human CRTh2: a combined homology modeling, molecular docking and 3D-QSAR-based in silico approach, Medicinal Chemistry Research, 2016, 25, 4, 653  crossref(new windwow)
G protein coupled receptor 18: A potential role for endocannabinoid signaling in metabolic dysfunction, Molecular Nutrition & Food Research, 2016, 60, 1, 92  crossref(new windwow)
M. D. Thompson, W. M. Burnham, and D. E. Cole, "The G protein-coupled receptors: pharmacogenetics and disease", Crit. Rev. Clin. Lab. Sci., Vol. 42, pp. 311-392, 2005. crossref(new window)

R. J. Lefkowitz, "Historical review: a brief history and personal retrospective of seven-transmembrane receptors", Trends Pharmacol., Sci., Vol. 25, pp. 413-422, 2004. crossref(new window)

S. Schlyer and R. Horuk, "I want a new drug: Gprotein-coupled receptors", Drug Discov. Today, Vol. 11, pp. 481-493, 2006. crossref(new window)

M. Kohno, H. Hasegawa, A. Inoue, M. Muraoka, T. Miyazaki, K. Oka, and M. Yasukawa, "Identification of N-arachidonylglycine as the endogenous ligand for orphan G-protein-coupled receptor GPR18", Biochem. Biophys. Res. Commun., Vol. 347, pp. 827-832, 2006. crossref(new window)

G. Kothandan, T. Madhavan, C. G. Gadhe, and S. J. Cho, "Pseudoreceptor: Concept and an overview", J. Chosun Natural Sci., Vol. 5, pp. 216-219, 2012. crossref(new window)

G. Kothandan and S. J. Cho, "Prediction of binding free energy calculation using molecular mechanics/ Poisson-Boltzmann surface area (MM-PBSA) method in drug discovery: A short review", J. Chosun Natural Sci., Vol. 2, pp. 55-58, 2009.

S. J. Cho, "Search space reduction techniques in small molecular docking", J. Chosun Natural Sci. Vol. 3, pp. 143-147, 2010.

S. J. Cho, "Calculation and application of partial charges", J. Chosun Natural Sci., Vol. 3, pp. 226-230, 2010.

S. J. Cho, "Meaning and definition of partial charges", J. Chosun Natural Sci., Vol. 3, pp. 231-236, 2010.

S. F. Altschul, W. Gish, W. Miller, E. W. Myers, and D. J. Lipman, "Basic local alignment search tool", J. Mol. Biol., Vol. 215, pp 403-410, 1990. crossref(new window)

S. F. Altschul, T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman, "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res., Vol. 50, pp. 3389-3402, 1997.

H. M. Berman, J. Westbrook, Z. Feng, G. Gilliland, T. N. Bhat, H. Weissig, I. N. Shindyalov, and P. E. Bourne, "The protein data bank", Nucleic Acids Res., Vol. 28, pp. 235-242, 2000. crossref(new window)

J. D. Thompson, D. G. Higgins, and T. J. Gibson, "CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice", Nucleic Acids Res., Vol. 22, pp. 4673-4680, 1994. crossref(new window)

N. Eswar, B. John, N. Mirkovic, A. Fiser, V. A. Ilyin, U. Pieper, A. C. Stuart, M. A. Marti-Renom, M. S. Madhusudhan, B. Yerkovich, and A. Sali, "Tools for comparative protein structure modeling and analysis", Nucleic Acids Res., Vol. 31, pp. 3375-3380, 2003. crossref(new window)

A. Sali and T. L. Blundell, "Comparative protein modeling by satisfaction of spatial restraints", J. Mol. Biol., Vol. 234, pp. 779-815, 1993. crossref(new window)

A. Fiser, R. K. Do, and A. Sali, "Modeling of loops in protein structures", Protein Sci., Vol. 9, pp. 1753-1773, 2002.

R. A. Laskowski, M. W. MacArthur, D. S. Moss, and J. M. Thornton, "Procheck-a program to check the stereochemical quality of protein structures", J. Appl. Cryst., Vol. 26, pp. 283-291, 1993. crossref(new window)

C. Colovos and T.O. Yeates, "Verification of protein structures: patterns of nonbonded atomic interactions", Protein Sci., Vol. 2, pp. 1511-1519, 1993. crossref(new window)

A. A. Thompson, W. Liu, E. Chun, V. Katritch, H. Wu, E. Vardy, X. P. Huang, C. Trapella, R. Guerrini, G. Clo, B. L. Roth, V. Cherezov, and R. C. Stevens, "Structure of the nociceptin/orphanin FQ receptor in complex with a pepti de mimetic", Nature, Vol. 485, pp. 395-399, 2012. crossref(new window)

Y. Qin, E. M. E. Verdegaal, M. Siderius, J. P. Bebelman, M. J. Smit, R. Leurs, R. Willemze, C. P. Tensen, and S. Osanto, "Quantitative expression proling of G-protein-coupled receptors (GPCRs) in metastatic melanoma: the constitutively active orphan GPCR GPR18 as novel drug target", Pig. Cell Melanoma R., Vol. 24, pp. 207-218, 2010.