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Detection of CTX-M and TEM type extended-spectrum β-lactamases in Escherichia coli isolated from livestocks in Korea
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 Title & Authors
Detection of CTX-M and TEM type extended-spectrum β-lactamases in Escherichia coli isolated from livestocks in Korea
Cho, Jae-Keun; Sung, Myung-Suk; Kim, Jin-Hyun; Kim, Ki-Seuk;
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This study was conducted to investigate the prevalence and genotypes of extended-spectrum -lactamase (ESBL) in 377 Escherichia coli isolated from healthy and sick animals. Two isolates (0.5%), each of which were isolated from diseased swine and chicken, respectively, were confirmed as ESBL producing isolates by double disk synergy test, and showed a multidrug resistant phenotype. Minimum inhibitory concentration of cefotaxime for the two ESBL producing isolates were 3~4 times higher than those of ceftazidime, respectively. By PCR and sequencing, one isolate from swine have both and , and one isolate from chicken have and . Also, these genes were transferred to E. coli J53 by conjugation. These two isolates showed unrelated pulsed-field gel electrophoresis. To our knowledge, this is the first time that gene was identified in E. coli isolated from animals in Korea. These results suggest more prudent use of third- generation cephalosporins, and surveillance and monitoring for ESBL producing E. coli in both animals and their environments should be necessary.
Escherichia coli;ESBL;;;;
 Cited by
대구지역 폐수처리장에서 분리한 cefotaxime-resistant Escherichia coli의 특성,김환득;박대현;이미리;김은정;조재근;

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