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Detection of CTX-M and TEM type extended-spectrum β-lactamases in Escherichia coli isolated from livestocks in Korea
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 Title & Authors
Detection of CTX-M and TEM type extended-spectrum β-lactamases in Escherichia coli isolated from livestocks in Korea
Cho, Jae-Keun; Sung, Myung-Suk; Kim, Jin-Hyun; Kim, Ki-Seuk;
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 Abstract
This study was conducted to investigate the prevalence and genotypes of extended-spectrum -lactamase (ESBL) in 377 Escherichia coli isolated from healthy and sick animals. Two isolates (0.5%), each of which were isolated from diseased swine and chicken, respectively, were confirmed as ESBL producing isolates by double disk synergy test, and showed a multidrug resistant phenotype. Minimum inhibitory concentration of cefotaxime for the two ESBL producing isolates were 3~4 times higher than those of ceftazidime, respectively. By PCR and sequencing, one isolate from swine have both and , and one isolate from chicken have and . Also, these genes were transferred to E. coli J53 by conjugation. These two isolates showed unrelated pulsed-field gel electrophoresis. To our knowledge, this is the first time that gene was identified in E. coli isolated from animals in Korea. These results suggest more prudent use of third- generation cephalosporins, and surveillance and monitoring for ESBL producing E. coli in both animals and their environments should be necessary.
 Keywords
Escherichia coli;ESBL;;;;
 Language
Korean
 Cited by
1.
대구지역 폐수처리장에서 분리한 cefotaxime-resistant Escherichia coli의 특성,김환득;박대현;이미리;김은정;조재근;

한국가축위생학회지, 2014. vol.37. 4, pp.225-231 crossref(new window)
2.
서울지역 유통 식육 중의 세팔로스포린계 항균물질 잔류실태 조사,김미란;최윤화;최훈;김두환;김영섭;이주형;

한국가축위생학회지, 2015. vol.38. 4, pp.259-264 crossref(new window)
1.
Prevalence of antimicrobial resistance and integrons in extended-spectrum β-lactamases producing Escherichia coli isolated from Nakdong and Gumho river, Korean Journal of Veterinary Service, 2014, 37, 1, 19  crossref(new windwow)
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Characterization of cefotaxime-resistant Escherichia coli isolated from wastewater treatment plant in Daegu, Korean Journal of Veterinary Service, 2014, 37, 4, 225  crossref(new windwow)
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Monitoring for cephalosporins residues in raw meat in Seoul, Korean Journal of Veterinary Service, 2015, 38, 4, 259  crossref(new windwow)
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