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Prevalence of the antimicrobial resistance and resistance associated gene in Salmonella spp. isolated from pigs and cattle in slaughterhouse
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Prevalence of the antimicrobial resistance and resistance associated gene in Salmonella spp. isolated from pigs and cattle in slaughterhouse
Hah, Do-Yun; Ji, Dae-Hae; Jo, Sang-Rae; Park, Ae-Ra; Jung, Eun-Hee; Park, Dong-Yeop; Lee, Kuk-Cheon; Yang, Jung-Wung; Kim, Jong-Shu; Kim, Hye-Jung; Jung, Jong-Hwa; Song, Ick-Hyun; Kim, Ae-Ran; Lee, Ji-Youn; Kim, Young-Hwan;
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This study was conducted to investigate the distribution of Salmonella spp. from pigs and cattle in slaughterhouse, the antimicrobial resistance pattern and the prevalence of resistance genes of isolates. A total of 640 fecal samples from pigs and cattle in slaughterhouse were collected for isolation of Salmonella spp.. Isolation rate was revealed as 15% in pigs and 1.6% in cattle. As result of serotyping, group B (56.6%) were identified as most common in pigs and cattle isolates, in order of group C (24.5%) and group E (15.1%). S. Typhimurium (50.9%) was most common serotype. The major serotypes were in order of S. Rissen and S. London (11.3%) and S. Riggil (7.6%). In antimicrobial test, all isolates were demonstrates susceptibility to nitrofurantoin. But isolates were revealed resistance other antibiotics in order of tetracycline (64.6%), streptomycin (68.3%), ampicillin and amoxicillin (56.3%) and spectinomycin (47.9%). With polymerase chain reaction, antimicrobial resistance gene strA (75.0%) and aadA1 (3.1%) were detected in streptomycin resistance isolates and tetA (94.3%) and tetB (11.3%) gene were detected in tetracycline resistant isolates, but tetG was not detected. Class 1 integron gene was detected in all Salmonella isolates.
Salmonella;Multidrug resistant;Resistance gene;Slaughterhouse;
 Cited by
양돈장 살모넬라 혈청 양성율과 도축돈 살모넬라균 분리율의 상관관계 및 분리 균주의 항생제 내성,김경언;안종민;양병훈;박영희;박미영;정준용;

한국가축위생학회지, 2014. vol.37. 4, pp.233-240 crossref(new window)
Correlation between the Salmonella seroprevalence on farms and the isolation rate from slaughtered pigs and antimicrobial resistance from the isolates, Korean Journal of Veterinary Service, 2014, 37, 4, 233  crossref(new windwow)
국립수의과학검역원. 2010. 축산물의 가공기준 및 성분규격. 161-182.

김상윤. 2000. 경북지역 가축에서 분리된 Salmonella속 균의 역학적 특성 및 병원성. 안동대학교 대학원 박사학위논문. 26-27.

강호조, 손원근, 1999. 한우 사육장내 Salmonella 속균의 존재 관련요인 분석. 한국수의공중보건학회지 23(2): 121-126.

이우원, 정병열, 이강록, 이동수, 김용환, 2009a. 소와 돼지유래 Salmonella속 균의 혈청형 및 약제 감수성. 한국가축위생학회지 32(1): 49-59.

이우원, 정병열, 이강록, 이동수, 김용환, 2009b. 소와 돼지유래 살모넬라속 균의 약제내성유전자의 특성에 관한 연구. 한국가축위생학회지 32(3): 227-239.

崔源弼, 李熙碩, 呂相建, 李憲俊, 鄭錫贊, 1986. 養豚場에서 살모넬라感染症의 疫學的 硏究: I. 發生 및 汚染狀況, 血淸型과 Salmonella typhimurium의 生物型. 大韓獸醫學會誌 26(1): 49-59.

崔源弼, 李熙碩, 呂相建, 李憲俊, 蔡太喆, 1988. 牛, 豚에서 分離한 Salmonella由來 R plasmid의 遺傳學的 및 分子生物學的 性狀에 관한 硏究 I. 乳牛에서 Salmonella屬菌의 分布狀況 및 藥劑耐性. 大韓獸醫學會誌 28(2):331-337.

Baggesen DL, Sandvang D, Aarestrup FM. 2000. Charaterization of Salmonella enterica serovar Typhimurium DT104 isolated from Denmark and comparison with isolates from Europe and the United States. J Clin Mirobiol 38(4): 1581-1586.

Bauer AW, Kirby WM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45(4): 493-496.

Bryant MC. 1972. Antibiotics and their Laboratory control. 2nd ed. Butterworth, London. : 41-45

Chiu CH, Tang P, Chu C, Hu S, Bao Q, Yu J, Chou YY, Wang HS, Lee YS, 2005. The genome sequence of Salmonella enterica serovar Choleraesuis, a highly invasive and resistant zoonotic pathogen. Nucleic Acids Res 33(5): 1690-1698. crossref(new window)

Davies RH, Dalziel R, Gibbens JC, Wilesmith JW. Ryan JM, Evans SJ, Byrne C, Paiba GA, Pascoe SJ, Teale CJ, 2004. National survey for Salmonella in pigs, cattle and sheep at slaughter in Great Britain (1999-2000). J Appl Microbiol 96(4): 750-760. crossref(new window)

Edrington TS, Schultz CL, Bischoff KM, Callaway TR, Looper ML, Genovese KJ, Jung YS, McReynolds JL, Anderson RC, Nisbet DJ, 2004. Antimicrobial resistance and serotype prevalence of Salmonella isolated from dairy cattle in the southwestern United States. Microb Drug Resist 10(1): 51-56. crossref(new window)

Edwards PR, Ewing WH. 1986. Identification of Enterobacteriaceae. 4eds. Elsevier Science Pub. Co., New York: 17-21.

Esaki H, Morioka A, Ishhara K, Kojima A, Shiroki S, Tamura Y, Takahashi T, 2004. Antimicrobial susecptibilies of Salmonella isolated from cattle, swineand poultry (2001-2002): reprot from Japanese Veterinary Antimicrobial Resistance Monitoring Program. J Antimicrob Chemother 53(2): 266-270. crossref(new window)

Gahring LC, Heffron F, Finlay BB, Falkow S, 1990. Invasion and replication of Salmonella typhimurium in animal cells. Infect Immun 58(2): 443-448.

Gebreyes WA, Thakur S, Davies PR, Funk JA, Altier C, 2004. Trends in antimicrobial resistance, phage types and integrons among Salmonella serotypes from pigs, 1997-2000. J Antimicrob Chemother 53(6): 997-1003. crossref(new window)

Gebreyes WA, Thakur S. 2005. Multidrug-resistant Salmonella enterica serovar Muenchen from pigs and humans and potential interserovar transfer of antimicrobial resistance. Antimicrob Agents Chemother 49(2): 503-511. crossref(new window)

Goldstein C, Lee MD, Sanchez S, Hudson C, Phillips B, Register B, Grady M, Liebert C, Summers AO, White DG, Maurer JJ, 2001. Incidence of class 1 and 2 integrases in clinical and commensal bacteria from livesock, companion animals, and exotics. Antimicro Agents Chemother 45(3): 723-736. crossref(new window)

Leverstein-Van Hall MA, Paauw A, Box AT, Blok HE, Verhoef J, Fluit AC. 2002. Presence of integron-associated resistance in the community is widespread and contributes to multidrug resistance in the hospital. J Clin Microbiol 40(8): 3038-3040. crossref(new window)

Kwang J, Littledike ET, Keen JE. 1996. Use of the polymerase chain reaction for Salmonella detection. Lett Appl Microbiol 22(1): 46-51. crossref(new window)

Madsen L, Aarestrup FM, Olsen JE. 2000. Characterisation of streptomycin resistance determinants in Danish isolates of Salmonella Typhimurium. Vet Microbiol 3;75(1): 73-82. crossref(new window)

Molorny B, Schoreter A, Bunge C, Hoog B, Steinbeck A, Helmuth R. 2001. Evaluation of Molecular typing methods for Salmonella enterica serovar Tyhimurium DT 104 isolated in Germany from healthy pigs. Vet Res 32(2): 119-129. crossref(new window)

National Committe for Clinical Laboratory Standards. 2002. Performance standards for antimicrobial discs and dilution susceptibility test for bacteria isolated from animals - second edtion : Approved standards M32-A2. NCCLS, Villanova, PA, USA.

Nemergut DR, Robeson MS, Kysela RF, Martin AP, Schmidt SK, Knight R. 2008. Insights and inferences about integron evolution from genomic data. BMC Genomics 31:9:261.

Pezzella C, Ricci A, DiGiannatale E, Luzzi I, Carattoli A. 2004. Tetracycline and streptomycin resistance genes, transposons, and plasmid in Salmonella enterica isolates from animals in Italy. Antimicrob Agents Chemother 48(3): 903-908. crossref(new window)

Randall LP. Cooles SW, Osborn MK, Piddock LJ, Woodward MJ. 2004. Antibiotic resistance genes, integrons and multiple antibiotic resistance in thirty five serotypes of Salmonella enterica isolated from humans and animals in the UK. J Antimicrob Chemother 53(2): 208-216. crossref(new window)

Sato G, Kodama H. 1974. Appearance of R-factor-mediated drug resistance in Salmonella Typhimurium excreted by carrier calves on a feedlot. Jpn J Vet Res 22(3): 72-79.

Swanenbrug M, Urlings HA, Snijders JM, Keuzenkamp DA, van Knapen F. 2001. Salmonella in slaughter pigs: prevalence, serotypes and critical control points during slaughter in two slaughterhouses. Int J Food Microbiol 70(3): 243-254. crossref(new window)

Tuchili LM, Kodama H, Sharma RN, Takatori I, Pandey GS, Kabilika S, Mukamoto M, Tsuji S, Baba T. 1996. Detection of Salmonella DNA in chicken embryos and environmental samples by polymerase chain reaction. J Vet Med Sci 58(9): 881-884. crossref(new window)

van Duijkeren EV, Wannet WJ, Houwers DJ, van Pelt W, 2002. Serotype and phage type distribution of Salmonella strains isolated from humans, cattle, pigs, and chickens in the Netherlands from 1984 to 2001. J Clin Microbiol 40(11): 3980-3985. crossref(new window)

van Essen-Zandbergen A, Smith H, Veldman K, Mevius D. 2007. Occurrence and characteristics of class 1, 2, and 3 integrons in Escherichia coli, Salmonella and Campylobacter spp. in the Netherlands. J Antimicrob Chemother 59(4): 746-750. crossref(new window)