JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Diagnosis on sudden death cases during summer season and isolation of Clostridium novyi
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Journal of Veterinary Service
  • Volume 39, Issue 2,  2016, pp.131-136
  • Publisher : The Korean Society of Veterinary Service
  • DOI : 10.7853/kjvs.2016.39.2.131
 Title & Authors
Diagnosis on sudden death cases during summer season and isolation of Clostridium novyi
Jeong, Chang-Gi; Seo, Byoung-Joo; Kim, Won-Il;
  PDF(new window)
 Abstract
Clostridium novyi (C. novyi) is a gram positive, non-capsulated, motile, obligatory anaerobe that produces endospores. Both C. novyi type A and B produce a bacteriophage encoded lethal alpha toxin which belongs to a family of large clostridial cytotoxins. These large clostridial cytotoxins of C. novyi bind to the uncharacterized receptors on host vascular endothelial cells, which leads to the loss of integrity of the vascular endothelium with subsequent edema, refractory hypotension, organ failure, and sudden death. A total of 13 sudden death cases were submitted to Chonbuk National University-Veterinary Diagnostic Center between June and October, 2015. The samples, mainly liver, were collected in sterile vials after necropsy and processed within 12~24 hours for diagnosis, isolation and identification of C. novyi. All of the 4 gram positive samples showed amplification by PCR. Out of 4 positive samples, 3 were detected to be C. novyi type B and 1 was detected as C. novyi type A. Based on the 16S rDNA sequence analysis, 1 case (150564) showed 99% similarity with C. novyi type A while other 3 cases (150388, 150557 and 150775) presented 99% similarity with C. novyi type B. Based on the results, C. novyi was found to be prevalent in Korean pig farms and causes sudden death to finishing pigs or sows during summer season. Thus, C. novyi should be considered for differential diagnosis on sudden death cases during the summer season.
 Keywords
Clostridium novyi;Sudden death cases;Summer season;Differentiation PCR;Flagellin genes;
 Language
Korean
 Cited by
 References
1.
Aronoff DM. 2013. Clostridium novyi, sordellii, and tetani:Mechanisms of disease. Anaerobe 24: 98-101. crossref(new window)

2.
Borrmann E, Schulze F. 1999. Detection of Clostridium novyi type B ${\alpha}$ toxin by cell culture systems. FEMS Immunology & Medical Microbiology 24(3): 275-280.

3.
Brazier JS, Duerden BI, Hall V, Salmon JE, Hood J, Brett MM, George RC. 2002. Isolation and identification of Clostridium spp. from infections associated with the injection of drugs: experiences of a microbiological investigation team. Journal of medical microbiology 51(11): 985-989. crossref(new window)

4.
Duran CO, Walton JR. 1997. Clostridium novyi sudden death in sows: toxaemia or post mortem invader? Pig Journal (United Kingdom).

5.
Eklund MW, Poysky FT, Peterson ME, Meyers JA. 1976. Relationship of bacteriophages to alpha toxin production in Clostridium novyi types A and B. Infection and immunity 14(3): 793-803.

6.
Garcia A, Ayuso D, Benitez JM, Garcia WL, Martinez R, Sanchez S. 2009. Clostridium novyi infection causing sow mortality in an Iberian pig herd raised in an outdoor rearing system in Spain. Journal of Swine Health and Production 17(5): 264-269.

7.
Sasaki Y, Takikawa N, Kojima A, Norimatsu M, Suzuki S, Tamura Y. 2001. Phylogenetic positions of Clostridium novyi and Clostridium haemolyticum based on 16S rDNA sequences. International journal of systematic and evolutionary microbiology 51(3): 901-904. crossref(new window)

8.
Sasaki Y, Kojima A, Aoki H, Ogikubo Y, Takikawa N, Tamura Y. 2002. Phylogenetic analysis and PCR detection of Clostridium chauvoei, Clostridium haemolyticum, Clostridium novyi types A and B, and Clostridium septicum based on the flagellin gene. Veterinary microbiology 86(3): 257-267. crossref(new window)

9.
Skarin H, Segerman B. 2014. Plasmidome interchange between Clostridium botulinum, Clostridium novyi and Clostridium haemolyticum converts strains of independent lineages into distinctly different pathogens. PloS one 9(9), e107777. crossref(new window)

10.
Smith L. DS. 1975. The pathogenic anaerobic bacteria. Charles C Thomas, Publisher, Springfield, Ill.