JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Evaluation of the virulence genes and Shiga toxin-producing abilities of Escherichia coli field isolates causing edema disease in pigs
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Evaluation of the virulence genes and Shiga toxin-producing abilities of Escherichia coli field isolates causing edema disease in pigs
Seo, Byoung-Joo; Jeong, Chang-Gi; Kang, A-Rum; Cho, Ho-Seong; Kim, Won-Il;
  PDF(new window)
 Abstract
Porcine edema disease (ED) is a communicable disease of pigs caused by infection with Shiga toxin (Stx)-producing Escherichia coli (STEC) which expresses F18 fimbriae and/or Stx type 2e (Stx2e). While STEC causes a severe illness including hemorrhagic colitis and hemolytic-uremic syndrome in humans, it induces damage to the vascular endothelium, which results in edema, hemorrhage, and microthrombosis, leading in high mortality in pigs. In the present study, we cultured Stx2e-producing E. coli field isolates from conventional pig farms that experienced sudden deaths previously with symptoms similar to porcine edema disease, which were further investigated with Shiga toxin profiles. A total of 43 strains were identified from the collected samples by F18 or Stx2e specific PCR. Based on the PCR, 42 isolates out of 43 isolates were proved to carry one of F18 or Stx2e genes and 14 isolates to carry both F18 and Stx2e genes. All of the 30 isolates that harbored Stx2e gene induced the cytopathic effect (CPE) in vero cells and especially, the isolate 150229 produced the highest level of Shiga toxin. Therefore, we identified the virulence genes (F18 and Stx2e) and demonstrated Shiga toxin-producing abilities from porcine edema disease causing E. coli filed isolates. These results suggested that one of the isolates could be a vaccine antigen candidate against STEC through further investigating to elicit an immune response.
 Keywords
Porcine edema disease;STEC;Shiga toxin;Cytotoxicity;F18;Stx;
 Language
Korean
 Cited by
 References
1.
변재원. 2015. 돼지 부종병의 원인과 대책. 월간한돈 2015년 12월호

2.
Bertin Y, Boukhors K, Pradel N, Livrelli V, Martin C. 2001. Stx2 subtyping of Shiga toxin-producing Escherichia coli isolated from cattle in France: detection of a new Stx2 subtype and correlation with additional virulence factors. J Clin Microbiol 39: 3060-3065. crossref(new window)

3.
Bettelheim KA, Beutin L. 2003. Rapid laboratory identification and characterization of verocytotoxigenic (Shiga toxin producing) Escherichia coli (VETC/STEC). J Appl Microbiol 95: 205-217. crossref(new window)

4.
Cho EJ, Kim DK, Kim SH, Kim YI, Lee CH, Lee WW, Son WG, Shin JU, Kim YH. 2005. Induction of Deletion Mutation for the Enzymatic Domain in the Shiga toxin 2e A Subunit Gene of Esherichila coli O139 Isolates and Expression of Mutated Protein. J Vet Clin 22: 386-391.

5.
Cornick NA, Matise I, Samuel JE, bosworth BT, Moon HW. 2000. Shiga Toxin-Producing Escherichia coli Infection: Temporal and Quantitative Relationships among Coloni zation, Toxin Production, and Systemic Disease. Journal of Infectious Diseases 181: 242-251. crossref(new window)

6.
Cummings MD, Ling H, Armstrong GD, Brunton JL, 1998. Read RJ. Modeling the carbohydrate-binding specificity of pig edema toxin. Biochem 37: 1789-1799. crossref(new window)

7.
Emerging Threats Quarterly Report, Pig diseases. 2014. AHVLA Regional Laboratories and SACCVS Veterinary Surveillance Centres, Quarterly Report 2014.

8.
Fraser ME, Fujinaga M, Cherney MM, Melton-Celsa AR, Twiddy EM, O'Brien AD, James MN. 2004. Structure of shiga toxin type 2 (Stx2) from Escherichia coli O157:H7. J Biol Chem 279: 27511-27517. crossref(new window)

9.
Gentry MK, Dalrymple JM. 1980. Quantitative microtiter cytotoxicity assay for Shigella toxin. J Clin Microbiol 12: 361-366.

10.
Imberechts H, De Greve H, Lintermans P. 1992. The pathogenesis of edema disease in pigs. A review. Vet Microbiol 31: 221-233. crossref(new window)

11.
Kim MJ, Kim SH, Kim TS, Kee HY, Seo JJ, Kim ES, Park JT, Chung JK, Lee JE. 2009. Identification of Shiga Toxin-producing E. coli Isolated from Diarrhea Patients and Cattle in Gwangju Area, Korea. J Bacteriol Virol 39: 29-39. crossref(new window)

12.
Lo AWH, Moonens K, Kerpel MD, Brys L, Pardon E, Remaut H, Greve HD. 2014. The Molecular Mechanism of Shiga Toxin Stx2e Neutralization by a Single-domain Antibody Targeting the Cell Receptor-binding Domain. J Biol Chem 289: 25374-25381. crossref(new window)

13.
MacLeod DL, Gyles CL, Wilcock BP. 1991. Reproduction of edema disease of swine with purified Shiga-like toxin-II variant. Vet Pathol 28: 66-73 crossref(new window)

14.
Matise I, Cornick NA, Samue JE, Moon HW. 2003. Binding of shiga toxin 2e to porcine erythrocytes in vivo and in vitro. Infect Immun 71: 5194-5201. crossref(new window)

15.
Oanh TK, Nguyen VK, De Greve H, Goddeeris BM. 2011. Protection of piglets against Edema disease by maternal immunization with Stx2e toxoid. Infect Immun 80: 469-473.

16.
Schmitt CK, McKee ML, O'Brien AD. 1991. Two copies of Shiga-like toxin II-related genes common in enterohemorrhagic Escherichia coli strains are responsible for the antigenic heterogeneity of the O157:H- strain E32511. Infect Immun 59: 1065-1073.

17.
Song HJ, Chai HS. 1998. Colibacillosis in domestic animals, a review. Korean J Vet Serv 21: 413-429.

18.
Tseng M, Fratamico PM, Manning SD, Funk JA. 2014. Shiga toxin-producing Escherichia coli in swine: the public health perspective. Animal Health Research Reviews 15: 63-75. crossref(new window)

19.
Takao T. Tanabe, T. Hong YM, Shimonishi Y, Kurazono H, Yutsudo T, Sasakawa C, Yoshikawa M, Takeda Y. 1988. Identity of molecular structure of Shiga-like toxin I (VT1) from Escherichia coli O157:H7 with that of Shiga toxin. Microb Pathog 5: 57-69.

20.
Zhang W, Zhao M, Ruescg L, Omot A, Francis D. 2007. Prevalence of virulence factors of Escherichia coli strains isolated from pigs with post-weaning diarrhea. Vet Microbiol 123: 145-152. crossref(new window)