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The Study of Estimation of Chromatin Abnormality of Ogye Rooster Sperm and Activity by Diff-Quik Staining Method
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  • Journal title : Korean Journal of Poultry Science
  • Volume 42, Issue 2,  2015, pp.109-116
  • Publisher : The Korean Society of Poultry Science
  • DOI : 10.5536/KJPS.2015.42.2.109
 Title & Authors
The Study of Estimation of Chromatin Abnormality of Ogye Rooster Sperm and Activity by Diff-Quik Staining Method
Kim, Sung Woo; Choi, Ahreum; Choe, Changyong; Kim, Dongkyo; Seong, Hwan-Hoo; Kim, Jae-Hwan; Kim, Chongdae;
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Ogye rooster sperm chromatin status can be detected using well established sperm assays. In this paper, a simple and fast method to monitor rooster sperm chromatin status could be employed in field for assessment of chicken sperm quality. Using standard bright field microscope, Diff-Quik stains can be reproducibly, easily and routinely monitored with simple staining. The presence of abnormal chromatin staining of rooster sperm was determined by darker stain in head. In the fresh semen, the viabilities of three tested Ogye spermatozoa were 93.53%, 82.42% and 90.63% and normal chromatin rates were 87.96%, 74.25% and 85.10% respectively. However, after freezing, the rates of viability of thawed semen were reduced to 69.58%, 61.98% and 72.20% and normal chromatin rate also reduced to 58.91%, 48.49% and 63.34%. A significant correlation between live sperm and normal sperm nuclei was 0.875 in fresh semen and 0.513 in frozen semen. After incubation of sperm at for 5min, the rates of viability, chromatin normality and sperm head activity were shown as , and in fresh semen. However, the rates of thawed semen were reduced to , and 47.32{\pm}5.02%, respectively. The relationship between chromatin normality and sperm head movements in fresh and thawed semen were 0.564 and 0.540, respectively. With these results, the chicken sperm normality could be assessed by the Diff-Quik staining that could be used for chromatin status of sperm head and activated morphology of live spermatozoa, as a simple and rapid staining method.
chicken sperm;morphology;viability;Diff-Quik staining;
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