Effect of Dietary-Liquid Resveratrol on Growth Performance, Meat Quality Traits, and Ileum Morphology of Broiler Chickens under Moderate Heat Stress Yi, Young-Joo; Yoo, Jaehong; Samiru, S. Wickramasuriya; Kim, Eunjoo; Koo, Bonjin; Heo, Jung Min;
A total of 144 one-day-old male broilers (ROSS Ross 308) () were used in a completely randomized design and allotted to one of three dietary treatments to give 6 replicates per treatment (8 birds per cage). The three watery dietary treatments were 1) a corn-soybean meal-based relation [Negative control (NC); no antimicrobial compounds added] with tab water, 2) 8 ppm liquid-alpha-lipoic acid [Positive control (PC)] or 3) 100 ppm liquid-resveratrol. All dietary treatments were continuously provided as a liquid form. Birds were housed in a battery cage (n=48), and were offered liquid dietary treatments at all times. The ambient temperature was maintained at for the first 3 weeks and decreased gradually to by the end of the experiment (d 35) to induce moderate heat stress. One bird per pen (n=6) was euthanized via cervical dislocation at day 21 to obtain terminal ileum to measure villous height and crypt depth, while another bird per pen (n=6) was similarly euthanized at day 35 and used to harvest breast meat and drumsticks to evaluate meat quality traits. Birds fed liquid-resveratrol did not improve (P>0.05) body weight and average daily gain compared with those fed other dietary treatments from day 1 to day 35. Birds fed liquid-resveratrol had no effect (P>0.05) on villous height compared with birds were in other dietary treatments at day 21. Liquid-resveratrol had no effect (P>0.05) on thiobarbituric acid reactive substance (TBARS) in drumsticks compared with other dietary treatments at day 35. Furthermore, birds fed liquid-resveratrol had no effect (P>0.05) on DPPH radical scavenging activity both in breast meat and drumsticks compared with those fed other dietary treatments at day 35. Morphological changes of ileum were not observed by immunofluorescence, and the level of occludin protein from ileum extracts also did not differ among groups. Our results suggested that liquid form of antioxidant compounds used in the current study such as alpha-lipoic acid (8 ppm) or resveratrol (100 ppm) did not improve growth performance, meat quality traits and histology of terminal ileum compared with those fed negative control in broiler chickens under moderate heat stress for 35 days.
Aengwanich W, Suttajit M 2010 Effect of polyphenols extracted from tamarind (Tamarindus indica L.) seed coat on physiological changes, heterophil/lymphocyte ratio, oxidative stress and body weight of broilers (Gallus domesticus) under chronic heat stress. Animal Science Journal 81(2): 264-270.
Bendall J, Swatland H 1988 A review of the relationships of ph with physical aspects of pork quality. Meat Science 24 (2):85-126.
Biewenga GP, Haenen GR, Bast A 1997 The pharmacology of the antioxidant lipoic acid. General Pharmacology. The Vascular System 29(3):315-331.
Blois, MS 1958 Antioxidant determinations by the use of a stable free radical. Nature 1199-1200.
Bonnet S, Geraert P, Lessire M, Carre B, Guillaumin S 1997 Effect of high ambient temperature on feed digestibility in broilers. Journal of Poultry Science 76(6):857-863.
Brand-Williams W, Cuvelier M, Berset C 1995 Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology 28(1):25-30.
Brewer MS, Ikins W, Harbers C 1992 Tba values, sensory characteristics, and volatiles in ground pork during long term frozen storage: Effects of packaging. Journal of Food Science 57(3):558-563.
Chen J, Tellez G, Richards JD, J Escobar 2015 Identification of potential biomarkers for gut barrier failure in broiler chickens. Frontiers in Veterinary Science 2:14.
Cummins PM 2012 Occludin: One protein, many forms. Molecular and Cellular Biology 32(2):242-250.
Deng W, Dong X, Tong J, Zhang Q 2012 The probiotic Bacillus licheniformis ameliorates heat stress-induced impairment of egg production, gut morphology, and intestinal mucosal immunity in laying hens. Journal of Poultry Science 91(3):575-582.
Deyhim F, Teeter R 1991 Research note: Sodium and potassium chloride drinking water supplementation effects on acid-base balance and plasma corticosterone in broilers reared in thermoneutral and heat-distressed environments. Journal of Poultry Science 70(12):2551-2553.
Frei B, Higdon JV 2003 Antioxidant activity of tea polyphenols in vivo: Evidence from animal studies. The Journal of Nutrition 133(10):3275S-3284S.
Gokce R, Akkus IM, Yontem M, Ay M, Gurel, Caglayan O, Bodur S, Ergun S 2000 Effects of dietary oils on lipoproteins, lipid peroxidation and thromboxane A2 production in chicks. Turkish Journal of Veterinary and Animal Sciences 24(5):473-478.
Halliwell B 1994 Free radicals, antioxidants, and human disease: Curiosity, cause, or consequence? The Lancet 344 (8924):721-724.
Hung LM, Chen JK, Huang SS, Lee RS, Su MJ 2000 Cardioprotective effect of resveratrol, a natural antioxidant derived from grapes. Cardiovascular Research 47(3):549-555.
Lambert G, Gisolfi CV, Berg DJ, Moseley PL, Oberley LW, Kregel KC 2002 Selected contribution: Hyperthermia-induced intestinal permeability and the role of oxidative and nitrosative stress. Journal of Applied Physiology 92(4): 1750-1761.
Lauridsen C, Nielsen JH, Henckel P, Sorensen MT 1999 Antioxidative and oxidative status in muscles of pigs fed rapeseed oil, vitamin E, and copper. Journal of Animal Science 77(1):105-115.
Lin H, Decuypere E, Buyse J 2006 Acute heat stress induces oxidative stress in broiler chickens. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 144(1):11-17.
Liu L, He J, Xie H, Yang Y, Li J, Zou Y 2014 Resveratrol induces antioxidant and heat shock protein mRNA expression in response to heat stress in black-boned chickens. Journal of Poultry Science 93(1):54-62.
Mahmoud K, Beck M, Scheideler S, Forman M, Anderson K, Kachman S 1996 Acute high environmental temperature and calcium-estrogen relationships in the hen. Journal of Poultry Science 75(12):1555-1562.
Mahmoud UT, Abdel-Rahman MAM, Darwish MHA, Applegate TJ, Cheng H-w 2015 Behavioral changes and feathering score in heat stressed broiler chickens fed diets containing different levels of propolis. Applied Animal Behaviour Science 166:98-105.
Packer L, Witt EH, Tritschler HJ 1995 Alpha-lipoic acid as a biological antioxidant. Free Radical Biology and Medicine 19(2):227-250.
Pluske JR, Hampson DJ, Williams IH 1997 Factors influencing the structure and function of the small intestine in the weaned pig: A review. Livestock Production Science 51(1):215-236.
Rice-Evans C, Miller N, Paganga G 1997 Antioxidant properties of phenolic compounds. Trends in Plant Science 2(4): 152-159.
Rubiolo J, Vega F 2008 Resveratrol protects primary rat hepatocytes against necrosis induced by reactive oxygen species. Biomedicine & Pharmacotherapy 62(9):606-612.
Seifried HE, Anderson DE, Fisher EI, Milner JA 2007 A review of the interaction among dietary antioxidants and reactive oxygen species. The Journal of Nutritional Biochemistry 18(9):567-579.
Smecuol E, Bai JC, Vazquez H, Kogan Z, Cabanne A, Niveloni S, Pedreira S, Boerr L, Maurino E, Meddings JB 1997 Gastrointestinal permeability in celiac disease. Gastroenterology 112(4):1129-1136.
Smith M 2003 Effects of different levels of zinc on the performance and immunocompetence of broilers under heat stress. Journal of Poultry Science 82(10):1580-1588.
Song J, Xiao K, Ke YL, Jiao LF, Hu CH, Diao QY, Shi B, Zou XT 2014 Effect of a probiotic mixture on intestinal microflora, morphology, and barrier integrity of broilers subjected to heat stress. Journal of Poultry Science 93(3): 581-588.
Srilatha T, Redely V, Qudratullah S, Raju M 2010 Effect of alpha-lipoic acid and vitamin e in diet on the performance, antioxidation and immune response in broiler chicken. Int Journal of Poultry Science 9:678-683.
Sturniolo GC, Fries W, Mazzon E, Di V Leo, Barollo M, D'inca R 2002 Effect of zinc supplementation on intestinal permeability in experimental colitis. Journal of Laboratory and Clinical Medicine 139(5):311-315.
Viveros A, Chamorro S, Pizarro M, Arija I, Centeno C, Brenes A 2011 Effects of dietary polyphenol-rich grape products on intestinal microflora and gut morphology in broiler chicks. Journal of Poultry Science 90(3):566-578.
Witte VC, Krause GF, Baley ME 1970 A new extraction method for determining 2 thiobarbituric acid values of pork and beef during storage. Journal of Food Science 35(5): 582-585.
Zhang B, Guo Y 2009 Supplemental zinc reduced intestinal permeability by enhancing occludin and zonula occludens protein-1 (ZO-1) expression in weaning piglets. British Journal of Nutrition 102(5):687-693.