Effects of Coenzyme Q10 on the Expression of Genes involved in Lipid Metabolism in Laying Hens Jang, In Surk; Moon, Yang Soo;
The aim of this study was to investigate the expression patterns of key genes involved in lipid metabolism in response to dietary Coenzyme Q10 (CoQ10) in hens. A total of 36 forty week-old Lohmann Brown were randomly allocated into 3 groups consisting of 4 replicates of 3 birds. Laying hens were subjected to one of following treatments: Control (BD, basal diet), T1 (BD+ CoQ10 100 mg/kg diet) and T2 (BD+ micellar of CoQ10 100 mg/kg diet). Birds were fed ad libitum a basal diet or the basal diet supplemented with CoQ10 for 5 weeks. Total RNA was extracted from the liver for quantitative RT-PCR. The mRNA levels of HMG-CoA reductase(HMGCR) and sterol regulatory element-binding proteins(SREBP)2 were decreased more than 30~50% in the liver of birds fed a basal diet supplemented with CoQ10 (p<0.05). These findings suggest that dietary CoQ10 can reduce cholesterol levels by the suppression of the hepatic HMGCR and SREBP2 genes. The gene expressions of liver X receptor (LXR) and SREBP1 were down regulated due to the addition of CoQ10 to the feed (p<0.05). The homeostasis of cholesterol can be regulated by LXR and SREBP1 in cholesterol-low-conditions. The supplement of CoQ10 caused a decreased expression of lipid metabolism-related genes including , XBP1, FASN, and GLUTs in the liver of birds (p<0.05). These data suggest that CoQ10 might be used as a dietary supplement to reduce cholesterol levels and to regulate lipid homeostasis in laying hens.
Albano CB, Muralikrishnan D, Ebadi M 2002 Distribution of coenzyme Q homologues in brain. Neurochem Res 27(5): 359-368.
Battino M, Ferri E, Gorini A, Villa RF, Rodriguez Huertas JF, Fiorella P, Genova ML, Lenaz G, Marchetti M 1990 Natural distribution and occurrence of coenzyme Q homologues. Membr Biochem 9(3):179-190.
Beyer RE 1992 An analysis of the role of coenzyme Q in free radical generation and as an antioxidant. Biochem Cell Biol 70(6):390-403.
Bhagavan HN, Chopra RK 2006 Coenzyme Q10: Absorption, tissue uptake, metabolism and pharmacokinetics. Free Radic Res 40(5):445-453.
Carver FM, Shibley IA Jr, Pennington JS, Pennington SN 2001 Differential expression of glucose transporters during chick embryogenesis. Cell Mol Life Sci 58(4):645-652.
Cook F, Briggs GM 1977 Egg Science and Technology, second ed., eds. Stadelman WJ and Cotterill OJ, Avi Publishing Company, Westport, pp. 92-108.
Elkin RG, Zhong Y, Donkin SS, Hengstschlager-Ottnad E, Schneider WJ 2006 Effects of atorvastatin on lipid metabolism in normolipidemic and hereditary hyperlipidemic, non-laying hens. Comp Biochem Physiol B Biochem Mol Biol 143(3):319-329.
Foufelle F, Ferre P 2002 New perspectives in the regulation of hepatic glycolytic and lipogenic genes by insulin and glucose: A role for the transcription factor sterol regulatory element binding protein-1c. Biochem J 366:377-391.
Geng AL, Guo YM 2005 Effects of dietary coenzyme Q10 supplementation on hepatic mitochondrial function and the activities of respiratory chain-related enzymes in ascitic broiler chickens. Br Poult Sci 46(5):626-634.
Groneberg DA, Kindermann B, Althammer M, Klapper M, Vormann J, Littarru GP, Doring F 2005 Coenzyme Q10 affects expression of genes involved in cell signalling, metabolism and transport in human CaCo-2 cells. Int J Biochem Cell Biol 37(6):1208-1218.
Honda K, Kamisoyama H, Motoori T, Saneyasu T, Hasegawa S 2010 Effect of dietary coenzyme Q10 on cholesterol metabolism in growing chickens. J Poult Sci 47:41-47.
Honda K, Saneyasu T, Motoki T, Park Y, Kamisoyama H 2013 Dietary coenzyme Q10 suppressed hepatic hydroxymethylglutaryl-CoA reductase activity in laying hens. Biosci Biotechnol Biochem 77(7):1572-1574.
Jang IS, Moon YS 2015 Effects of lycopene on the expression of lipid metabolism, glucose transport and pro-inflammatory related genes in chickens. Korean J Poult Sci 42:231-238.
Kalen A, Norling B, Appelkvist EL, Dallner G 1987 Ubiquinone biosynthesis by the microsomal fraction from rat liver. Biochim Biophys Acta 926(1):70-78.
Kamisoyama H, Honda K, Kitaguchi K, Hasegawa S 2010 Transfer of dietary coenzyme Q10 into the egg yolk of laying hens. J Poult Sci 47:28-33.
Kono T, Nishida M, Nishiki Y, Seki Y, Sato K, Akiba Y 2005 Characterization of glucose transporter (GLUT) gene expression in broiler chickens. Br Poult Sci 46(4):510-515.
Krishnaiah KV, Ramasarma T 1970 Regulation of hepatic cholesterolgenesis by ubiquinone. Biochim Biophys Acta 202(2):332-342.
Lee AH, Scapa EF, Cohen DE, Glimcher LH 2008 Regulation of hepatic lipogenesis by the transcription factor XBP1. Science 320(5882):1492-1496.
Livak KJ, Schmittgen TD 2001 Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) method. Methods 25(4):402-408.
Maxwell MH, Robertson GW 1998 UK survey of broiler ascites and sudden death syndromes in 1993. Br Poult Sci 39(2):203-215.
Modi K, Santani DD, Goyal RK, Bhatt PA 2006 Effect of coenzyme Q10 on catalase activity and other antioxidant parameters in streptozotocin-induced diabetic rats. Biol Trace Elem Res 109(1):25-34.
Nakamura Y, Okamura T, Tamaki S, Kadowaki T, Hayakawa T, Kita Y, Okayama A, Ueshima H 2004 Egg consumption, serum cholesterol, and cause-specific and all-cause mortality: The national integrated project for prospective observation of non-communicable disease and its trends in the aged, 1980 (NIPPON DATA80). Am J Clin Nutr 80: 58-63.
O'Hea EK, Leveille GA 1968 Lipogenesis in isolated adipose tissue of the domestic chick (Gallus domesticus). Comp Biochem Physiol 26(1):111-120.
SAS 1996 User's Guide: Statistics Version 6.12 Ed. SAS Inst., Inc., Cary, NC.
Saneyasu T, Shiragaki M, Nakanishi K, Kamisoyama H, Honda K 2013 Effects of short term fasting on the expression of genes involved in lipid metabolism in chicks. Comp Biochem Physiol B Biochem Mol Biol 165(2):114-118.
Sato K, Kamada T 2011 Regulation of bile acid, cholesterol, and fatty acid synthesis in chicken primary hepatocytes by different concentrations of T0901317, an agonist of liver X receptors. Comp Biochem Physiol A Mol Integr Physiol 158(2):201-206.
Schadinger SE, Bucher NL, Schreiber BM, Farmer SR 2005 PPARgamma2 regulates lipogenesis and lipid accumulation in steatotic hepatocytes. Am J Physiol Endocrinol Metab 288(6):E1195-1205.