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Use of Molecular Detection Technique for Red Tide Warning of Cochlodinium polykrikoides
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  • Journal title : The Sea
  • Volume 21, Issue 1,  2016, pp.44-47
  • Publisher : The Korean Society of Oceanography
  • DOI : 10.7850/jkso.2016.21.1.44
 Title & Authors
Use of Molecular Detection Technique for Red Tide Warning of Cochlodinium polykrikoides
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Real-time PCR was applied to early warning of red tide. For early warning of red tide at , Cochlodinium polykrikoides specific real-time PCR was used as a complement of microscopy that has a lower detection sensitivity. C. polykrikoides appeared extensively in Tongyeong, Namhae waters at low densities in the end of June, and early warning of C. polyrkrikoides blooms was issued on 2 August 2015.
Cochlodinium;Harmful algae;Red tide;Real-time PCR;
 Cited by
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Coyne, K., Handy, S.H., Demir, E., Whereat, E.B., Hutchins, D.A., Portune, K.J., Doblin, M.A. and Craig, C.S., 2005. Improved quantitative real-time PCR assays for enumeration of harmful algal species in field samples using an exogenous DNA reference standard. Limnol. Oceanogr. Meth. 3: 381-391. crossref(new window)

Handy, S.M., Demir, E., Hutchins, D.A., Portune, K.J., Whereat, E.B., Hare, C., Rose, J., Warner, M., Farestad, M. and Cary, S.C., 2008. Using quantitative real-time PCR to study competition and community dynamics among Delaware Inland Bays harmful algae in field and laboratory studies. Harmful Algae 7: 599-613. crossref(new window)

Lee, C.K., Park, T.G., Park Y.T. and Lim, W.A. 2013. Monitoring and trends in red-tide events in Korea with emphasis on Cochlodinium polykrikoides. Harmful Algae 30S: 3-14.

Park, T.G., de Salas, M.F., Bolch, C.J.S. and Hallegraeff, G.M., 2007. Development of a real-time PCR probe for quantification of the heterotrophic dinoflagellate Cryptoperidiniopsis brodyi (Dinophyceae) in environmental samples. Appl. Environ. Microbiol. 73: 2552-2560. crossref(new window)

Park, T.G., Park, G.H., Park, Y.T., Kang, Y.S., Bae, H.M., Kim, C.H., Jeong, H.J. and Lee, Y., 2009a. Identification of the dinoflagellate community during Cochlodinium polykrikoides (Dinophyceae) blooms using amplified rDNA melting curve analysis and realtime PCR probes. Harmful Algae 8: 430-440. crossref(new window)

Park, T.G., Park, Y.T. and Lee Y., 2009b. Development of a SYTO9 based real-time PCR probe for detection and quantification of toxic dinoflagellate Karlodinium veneficum (Dinophyceae) in environmental samples. Phycologia 48: 32-43. crossref(new window)

Park, T.G. and Park, Y.T., 2010. Detection of Cochlodinium polykrikoides and Gymnodinium impudicum (Dinophyceae) in sediment samples from Korea using real-time PCR. Harmful Algae 9: 59-65. crossref(new window)

Park, T.G., Lim, W.A., Park, Y.T., Lee, C.K. and Jeong, H.J., 2013. Economic impact, management and mitigation of red tides in Korea. Harmful Algae 30S: 131-143.